RNA-Seq-mediated transcriptomic analysis of heat stress response in a polar Chlorella sp. (Trebouxiophyceae, Chlorophyta)
The current outlook on mitigation of global warming does not appear promising, with figures in the reduction of anthropogenic greenhouse gas emissions lagging far behind climate goals. A recent environmental report even postulated a high possibility of temperature increase of at least 3 °C by 2100....
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ftunivmalaya:oai:eprints.um.edu.my:21016 2023-05-15T13:54:20+02:00 RNA-Seq-mediated transcriptomic analysis of heat stress response in a polar Chlorella sp. (Trebouxiophyceae, Chlorophyta) Poong, Sze Wan Lee, Kok Keong Lim, Phaik Eem Pai, Tun Wen Wong, Chiew Yen Phang, Siew Moi Chen, Chien Ming Yang, Cing Han Liu, Chun Cheng 2018 http://eprints.um.edu.my/21016/ https://doi.org/10.1007/s10811-018-1455-9 unknown Springer Verlag Poong, Sze Wan and Lee, Kok Keong and Lim, Phaik Eem and Pai, Tun Wen and Wong, Chiew Yen and Phang, Siew Moi and Chen, Chien Ming and Yang, Cing Han and Liu, Chun Cheng (2018) RNA-Seq-mediated transcriptomic analysis of heat stress response in a polar Chlorella sp. (Trebouxiophyceae, Chlorophyta). Journal of Applied Phycology, 30 (6). pp. 3103-3119. ISSN 0921-8971 Q Science (General) QH Natural history Article PeerReviewed 2018 ftunivmalaya https://doi.org/10.1007/s10811-018-1455-9 2019-04-23T15:07:46Z The current outlook on mitigation of global warming does not appear promising, with figures in the reduction of anthropogenic greenhouse gas emissions lagging far behind climate goals. A recent environmental report even postulated a high possibility of temperature increase of at least 3 °C by 2100. Despite the low number of human inhabitants in Antarctica, the Antarctic Peninsula was reported as one of the most rapidly warming locations on earth. Many studies have shown that heat stress modulates physiological performance in many species of microalgae; however, studies to elucidate the molecular mechanisms of high-temperature thermotolerance are generally focused on the model species, i.e. Chlamydomonas reinhardtii. Furthermore, previous transcriptomic work in this aspect generally employed the microarray technique and/or involved the tropical or temperate strains, and few were conducted on the polar strains. In this study, RNA-Seq-mediated transcriptomic analysis was undertaken to compare the whole transcriptome profile of an Antarctic Chlorella sp. grown at ambient (4 °C) versus stress-inducing high (33 °C) temperatures and harvested at the 120-h time point. The findings of this study indicated a coordinated response to fine tune balance between energy production and utilisation for biosynthesis by redirecting carbon provision, and the arrest of cell division as a coping mechanism for an intense and relatively long period of stress. The strategies undertaken by this alga in acclimation to heat stress are somewhat similar to the heat stress response of the model species. Article in Journal/Newspaper Antarc* Antarctic Antarctic Peninsula Antarctica University of Malaya: UM Institutional Repository Antarctic Antarctic Peninsula The Antarctic Journal of Applied Phycology 30 6 3103 3119 |
institution |
Open Polar |
collection |
University of Malaya: UM Institutional Repository |
op_collection_id |
ftunivmalaya |
language |
unknown |
topic |
Q Science (General) QH Natural history |
spellingShingle |
Q Science (General) QH Natural history Poong, Sze Wan Lee, Kok Keong Lim, Phaik Eem Pai, Tun Wen Wong, Chiew Yen Phang, Siew Moi Chen, Chien Ming Yang, Cing Han Liu, Chun Cheng RNA-Seq-mediated transcriptomic analysis of heat stress response in a polar Chlorella sp. (Trebouxiophyceae, Chlorophyta) |
topic_facet |
Q Science (General) QH Natural history |
description |
The current outlook on mitigation of global warming does not appear promising, with figures in the reduction of anthropogenic greenhouse gas emissions lagging far behind climate goals. A recent environmental report even postulated a high possibility of temperature increase of at least 3 °C by 2100. Despite the low number of human inhabitants in Antarctica, the Antarctic Peninsula was reported as one of the most rapidly warming locations on earth. Many studies have shown that heat stress modulates physiological performance in many species of microalgae; however, studies to elucidate the molecular mechanisms of high-temperature thermotolerance are generally focused on the model species, i.e. Chlamydomonas reinhardtii. Furthermore, previous transcriptomic work in this aspect generally employed the microarray technique and/or involved the tropical or temperate strains, and few were conducted on the polar strains. In this study, RNA-Seq-mediated transcriptomic analysis was undertaken to compare the whole transcriptome profile of an Antarctic Chlorella sp. grown at ambient (4 °C) versus stress-inducing high (33 °C) temperatures and harvested at the 120-h time point. The findings of this study indicated a coordinated response to fine tune balance between energy production and utilisation for biosynthesis by redirecting carbon provision, and the arrest of cell division as a coping mechanism for an intense and relatively long period of stress. The strategies undertaken by this alga in acclimation to heat stress are somewhat similar to the heat stress response of the model species. |
format |
Article in Journal/Newspaper |
author |
Poong, Sze Wan Lee, Kok Keong Lim, Phaik Eem Pai, Tun Wen Wong, Chiew Yen Phang, Siew Moi Chen, Chien Ming Yang, Cing Han Liu, Chun Cheng |
author_facet |
Poong, Sze Wan Lee, Kok Keong Lim, Phaik Eem Pai, Tun Wen Wong, Chiew Yen Phang, Siew Moi Chen, Chien Ming Yang, Cing Han Liu, Chun Cheng |
author_sort |
Poong, Sze Wan |
title |
RNA-Seq-mediated transcriptomic analysis of heat stress response in a polar Chlorella sp. (Trebouxiophyceae, Chlorophyta) |
title_short |
RNA-Seq-mediated transcriptomic analysis of heat stress response in a polar Chlorella sp. (Trebouxiophyceae, Chlorophyta) |
title_full |
RNA-Seq-mediated transcriptomic analysis of heat stress response in a polar Chlorella sp. (Trebouxiophyceae, Chlorophyta) |
title_fullStr |
RNA-Seq-mediated transcriptomic analysis of heat stress response in a polar Chlorella sp. (Trebouxiophyceae, Chlorophyta) |
title_full_unstemmed |
RNA-Seq-mediated transcriptomic analysis of heat stress response in a polar Chlorella sp. (Trebouxiophyceae, Chlorophyta) |
title_sort |
rna-seq-mediated transcriptomic analysis of heat stress response in a polar chlorella sp. (trebouxiophyceae, chlorophyta) |
publisher |
Springer Verlag |
publishDate |
2018 |
url |
http://eprints.um.edu.my/21016/ https://doi.org/10.1007/s10811-018-1455-9 |
geographic |
Antarctic Antarctic Peninsula The Antarctic |
geographic_facet |
Antarctic Antarctic Peninsula The Antarctic |
genre |
Antarc* Antarctic Antarctic Peninsula Antarctica |
genre_facet |
Antarc* Antarctic Antarctic Peninsula Antarctica |
op_relation |
Poong, Sze Wan and Lee, Kok Keong and Lim, Phaik Eem and Pai, Tun Wen and Wong, Chiew Yen and Phang, Siew Moi and Chen, Chien Ming and Yang, Cing Han and Liu, Chun Cheng (2018) RNA-Seq-mediated transcriptomic analysis of heat stress response in a polar Chlorella sp. (Trebouxiophyceae, Chlorophyta). Journal of Applied Phycology, 30 (6). pp. 3103-3119. ISSN 0921-8971 |
op_doi |
https://doi.org/10.1007/s10811-018-1455-9 |
container_title |
Journal of Applied Phycology |
container_volume |
30 |
container_issue |
6 |
container_start_page |
3103 |
op_container_end_page |
3119 |
_version_ |
1766260047380217856 |