AidP, a novel N-Acyl homoserine lactonase gene from Antarctic Planococcus sp.
Planococcus is a Gram-positive halotolerant bacterial genus in the phylum Firmicutes, commonly found in various habitats in Antarctica. Quorum quenching (QQ) is the disruption of bacterial cell-to-cell communication (known as quorum sensing), which has previously been described in mesophilic bacteri...
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ftunivmalaya:oai:eprints.um.edu.my:19033 2023-05-15T13:51:19+02:00 AidP, a novel N-Acyl homoserine lactonase gene from Antarctic Planococcus sp. See-Too, W.S. Ee, R. Lim, Y.L. Convey, P. Pearce, D.A. Yin, Wai Fong Chan, Kok Gan 2017 application/pdf http://eprints.um.edu.my/19033/ http://eprints.um.edu.my/19033/1/AidP,_a_novel_N-acyl_homoserine_lactonase_gene_from_Antarctic_Planococcus_sp.pdf https://doi.org/10.1038/srep42968 en eng Nature Publishing Group http://eprints.um.edu.my/19033/1/AidP,_a_novel_N-acyl_homoserine_lactonase_gene_from_Antarctic_Planococcus_sp.pdf See-Too, W.S. and Ee, R. and Lim, Y.L. and Convey, P. and Pearce, D.A. and Yin, Wai Fong and Chan, Kok Gan (2017) AidP, a novel N-Acyl homoserine lactonase gene from Antarctic Planococcus sp. Scientific Reports, 7 (1). p. 42968. ISSN 2045-2322 Q Science (General) QH Natural history Article PeerReviewed 2017 ftunivmalaya https://doi.org/10.1038/srep42968 2019-12-24T16:13:54Z Planococcus is a Gram-positive halotolerant bacterial genus in the phylum Firmicutes, commonly found in various habitats in Antarctica. Quorum quenching (QQ) is the disruption of bacterial cell-to-cell communication (known as quorum sensing), which has previously been described in mesophilic bacteria. This study demonstrated the QQ activity of a psychrotolerant strain, Planococcus versutus strain L10.15T, isolated from a soil sample obtained near an elephant seal wallow in Antarctica. Whole genome analysis of this bacterial strain revealed the presence of an N-acyl homoserine lactonase, an enzyme that hydrolyzes the ester bond of the homoserine lactone of N-acyl homoserine lactone (AHLs). Heterologous gene expression in E. coli confirmed its functions for hydrolysis of AHLs, and the gene was designated as aidP (autoinducer degrading gene from Planococcus sp.). The low temperature activity of this enzyme suggested that it is a novel and uncharacterized class of AHL lactonase. This study is the first report on QQ activity of bacteria isolated from the polar regions. Article in Journal/Newspaper Antarc* Antarctic Antarctica Elephant Seal University of Malaya: UM Institutional Repository Antarctic Scientific Reports 7 1 |
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University of Malaya: UM Institutional Repository |
op_collection_id |
ftunivmalaya |
language |
English |
topic |
Q Science (General) QH Natural history |
spellingShingle |
Q Science (General) QH Natural history See-Too, W.S. Ee, R. Lim, Y.L. Convey, P. Pearce, D.A. Yin, Wai Fong Chan, Kok Gan AidP, a novel N-Acyl homoserine lactonase gene from Antarctic Planococcus sp. |
topic_facet |
Q Science (General) QH Natural history |
description |
Planococcus is a Gram-positive halotolerant bacterial genus in the phylum Firmicutes, commonly found in various habitats in Antarctica. Quorum quenching (QQ) is the disruption of bacterial cell-to-cell communication (known as quorum sensing), which has previously been described in mesophilic bacteria. This study demonstrated the QQ activity of a psychrotolerant strain, Planococcus versutus strain L10.15T, isolated from a soil sample obtained near an elephant seal wallow in Antarctica. Whole genome analysis of this bacterial strain revealed the presence of an N-acyl homoserine lactonase, an enzyme that hydrolyzes the ester bond of the homoserine lactone of N-acyl homoserine lactone (AHLs). Heterologous gene expression in E. coli confirmed its functions for hydrolysis of AHLs, and the gene was designated as aidP (autoinducer degrading gene from Planococcus sp.). The low temperature activity of this enzyme suggested that it is a novel and uncharacterized class of AHL lactonase. This study is the first report on QQ activity of bacteria isolated from the polar regions. |
format |
Article in Journal/Newspaper |
author |
See-Too, W.S. Ee, R. Lim, Y.L. Convey, P. Pearce, D.A. Yin, Wai Fong Chan, Kok Gan |
author_facet |
See-Too, W.S. Ee, R. Lim, Y.L. Convey, P. Pearce, D.A. Yin, Wai Fong Chan, Kok Gan |
author_sort |
See-Too, W.S. |
title |
AidP, a novel N-Acyl homoserine lactonase gene from Antarctic Planococcus sp. |
title_short |
AidP, a novel N-Acyl homoserine lactonase gene from Antarctic Planococcus sp. |
title_full |
AidP, a novel N-Acyl homoserine lactonase gene from Antarctic Planococcus sp. |
title_fullStr |
AidP, a novel N-Acyl homoserine lactonase gene from Antarctic Planococcus sp. |
title_full_unstemmed |
AidP, a novel N-Acyl homoserine lactonase gene from Antarctic Planococcus sp. |
title_sort |
aidp, a novel n-acyl homoserine lactonase gene from antarctic planococcus sp. |
publisher |
Nature Publishing Group |
publishDate |
2017 |
url |
http://eprints.um.edu.my/19033/ http://eprints.um.edu.my/19033/1/AidP,_a_novel_N-acyl_homoserine_lactonase_gene_from_Antarctic_Planococcus_sp.pdf https://doi.org/10.1038/srep42968 |
geographic |
Antarctic |
geographic_facet |
Antarctic |
genre |
Antarc* Antarctic Antarctica Elephant Seal |
genre_facet |
Antarc* Antarctic Antarctica Elephant Seal |
op_relation |
http://eprints.um.edu.my/19033/1/AidP,_a_novel_N-acyl_homoserine_lactonase_gene_from_Antarctic_Planococcus_sp.pdf See-Too, W.S. and Ee, R. and Lim, Y.L. and Convey, P. and Pearce, D.A. and Yin, Wai Fong and Chan, Kok Gan (2017) AidP, a novel N-Acyl homoserine lactonase gene from Antarctic Planococcus sp. Scientific Reports, 7 (1). p. 42968. ISSN 2045-2322 |
op_doi |
https://doi.org/10.1038/srep42968 |
container_title |
Scientific Reports |
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7 |
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1 |
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1766255137950531584 |