High-CO2 Levels Rather than Acidification Restrict Emiliania huxleyi Growth and Performance
The coccolithophore Emiliania huxleyi shows a variety of responses to ocean acidification (OA) and to high-CO2 concentrations, but there is still controversy on differentiating between these two factors when using different strains and culture methods. A heavily calcified type A strain isolated from...
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Online Access: | https://hdl.handle.net/10630/24222 https://doi.org/10.1007/s00248-022-02035-3 |
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ftunivmalaga:oai:riuma.uma.es:10630/24222 2023-11-12T04:23:31+01:00 High-CO2 Levels Rather than Acidification Restrict Emiliania huxleyi Growth and Performance Vázquez Manzanares, Víctor León Díaz, Pablo Ignacio López-Gordillo, Francisco Javier Jimenez, Carlos Íñiguez, Concepción MacKenzie, Kevin Bresnan, Eileen Segovia-Azcorra, María 2022-05-27 https://hdl.handle.net/10630/24222 https://doi.org/10.1007/s00248-022-02035-3 eng eng Springer Vázquez, V., León, P., Gordillo, F.J.L. et al. High-CO2 Levels Rather than Acidification Restrict Emiliania huxleyi Growth and Performance. Microb Ecol (2022). https://doi.org/10.1007/s00248-022-02035-3 https://hdl.handle.net/10630/24222 https://doi.org/10.1007/s00248-022-02035-3 info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/4.0/ Atribución 4.0 Internacional Calcificación Emiliania huxleyi Ocean acidifcation Calcifcation pCO2 Photochemistry Phytoplankton Stress Coccolithophores info:eu-repo/semantics/article 2022 ftunivmalaga https://doi.org/10.1007/s00248-022-02035-3 2023-10-24T23:13:53Z The coccolithophore Emiliania huxleyi shows a variety of responses to ocean acidification (OA) and to high-CO2 concentrations, but there is still controversy on differentiating between these two factors when using different strains and culture methods. A heavily calcified type A strain isolated from the Norwegian Sea was selected and batch cultured in order to understand whether acclimation to OA was mediated mainly by CO2 or H+, and how it impacted cell growth performance, calcification, and physiological stress management. Emiliania huxleyi responded differently to each acidification method. CO2-enriched aeration (1200 µatm, pH 7.62) induced a negative effect on the cells when compared to acidification caused by decreasing pH alone (pH 7.60). The growth rates of the coccolithophore were more negatively affected by high pCO2 than by low pH without CO2 enrichment with respect to the control (400 µatm, pH 8.1). High CO2 also affected cell viability and promoted the accumulation of reactive oxygen species (ROS), which was not observed under low pH. This suggests a possible metabolic imbalance induced by high CO2 alone. In contrast, the affinity for carbon uptake was negatively affected by both low pH and high CO2. Photochemistry was only marginally affected by either acidification method when analysed by PAM fluorometry. The POC and PIC cellular quotas and the PIC:POC ratio shifted along the different phases of the cultures; consequently, calcification did not follow the same pattern observed in cell stress and growth performance. Specifically, acidification by HCl addition caused a higher proportion of severely deformed coccoliths, than CO2 enrichment. These results highlight the capacity of CO2 rather than acidification itself to generate metabolic stress, not reducing calcification. Open Access funding provided thanks to the CRUE-CSIC agreement with Springer Nature. Funding for open access charge: Universidad de Málaga / CBUA This work was funded by FC14-RNM-27 research grant (FITOVIA) from the University of ... Article in Journal/Newspaper Norwegian Sea Ocean acidification RIUMA - Repositorio Institucional de la Universidad de Málaga Norwegian Sea Microbial Ecology |
institution |
Open Polar |
collection |
RIUMA - Repositorio Institucional de la Universidad de Málaga |
op_collection_id |
ftunivmalaga |
language |
English |
topic |
Calcificación Emiliania huxleyi Ocean acidifcation Calcifcation pCO2 Photochemistry Phytoplankton Stress Coccolithophores |
spellingShingle |
Calcificación Emiliania huxleyi Ocean acidifcation Calcifcation pCO2 Photochemistry Phytoplankton Stress Coccolithophores Vázquez Manzanares, Víctor León Díaz, Pablo Ignacio López-Gordillo, Francisco Javier Jimenez, Carlos Íñiguez, Concepción MacKenzie, Kevin Bresnan, Eileen Segovia-Azcorra, María High-CO2 Levels Rather than Acidification Restrict Emiliania huxleyi Growth and Performance |
topic_facet |
Calcificación Emiliania huxleyi Ocean acidifcation Calcifcation pCO2 Photochemistry Phytoplankton Stress Coccolithophores |
description |
The coccolithophore Emiliania huxleyi shows a variety of responses to ocean acidification (OA) and to high-CO2 concentrations, but there is still controversy on differentiating between these two factors when using different strains and culture methods. A heavily calcified type A strain isolated from the Norwegian Sea was selected and batch cultured in order to understand whether acclimation to OA was mediated mainly by CO2 or H+, and how it impacted cell growth performance, calcification, and physiological stress management. Emiliania huxleyi responded differently to each acidification method. CO2-enriched aeration (1200 µatm, pH 7.62) induced a negative effect on the cells when compared to acidification caused by decreasing pH alone (pH 7.60). The growth rates of the coccolithophore were more negatively affected by high pCO2 than by low pH without CO2 enrichment with respect to the control (400 µatm, pH 8.1). High CO2 also affected cell viability and promoted the accumulation of reactive oxygen species (ROS), which was not observed under low pH. This suggests a possible metabolic imbalance induced by high CO2 alone. In contrast, the affinity for carbon uptake was negatively affected by both low pH and high CO2. Photochemistry was only marginally affected by either acidification method when analysed by PAM fluorometry. The POC and PIC cellular quotas and the PIC:POC ratio shifted along the different phases of the cultures; consequently, calcification did not follow the same pattern observed in cell stress and growth performance. Specifically, acidification by HCl addition caused a higher proportion of severely deformed coccoliths, than CO2 enrichment. These results highlight the capacity of CO2 rather than acidification itself to generate metabolic stress, not reducing calcification. Open Access funding provided thanks to the CRUE-CSIC agreement with Springer Nature. Funding for open access charge: Universidad de Málaga / CBUA This work was funded by FC14-RNM-27 research grant (FITOVIA) from the University of ... |
format |
Article in Journal/Newspaper |
author |
Vázquez Manzanares, Víctor León Díaz, Pablo Ignacio López-Gordillo, Francisco Javier Jimenez, Carlos Íñiguez, Concepción MacKenzie, Kevin Bresnan, Eileen Segovia-Azcorra, María |
author_facet |
Vázquez Manzanares, Víctor León Díaz, Pablo Ignacio López-Gordillo, Francisco Javier Jimenez, Carlos Íñiguez, Concepción MacKenzie, Kevin Bresnan, Eileen Segovia-Azcorra, María |
author_sort |
Vázquez Manzanares, Víctor |
title |
High-CO2 Levels Rather than Acidification Restrict Emiliania huxleyi Growth and Performance |
title_short |
High-CO2 Levels Rather than Acidification Restrict Emiliania huxleyi Growth and Performance |
title_full |
High-CO2 Levels Rather than Acidification Restrict Emiliania huxleyi Growth and Performance |
title_fullStr |
High-CO2 Levels Rather than Acidification Restrict Emiliania huxleyi Growth and Performance |
title_full_unstemmed |
High-CO2 Levels Rather than Acidification Restrict Emiliania huxleyi Growth and Performance |
title_sort |
high-co2 levels rather than acidification restrict emiliania huxleyi growth and performance |
publisher |
Springer |
publishDate |
2022 |
url |
https://hdl.handle.net/10630/24222 https://doi.org/10.1007/s00248-022-02035-3 |
geographic |
Norwegian Sea |
geographic_facet |
Norwegian Sea |
genre |
Norwegian Sea Ocean acidification |
genre_facet |
Norwegian Sea Ocean acidification |
op_relation |
Vázquez, V., León, P., Gordillo, F.J.L. et al. High-CO2 Levels Rather than Acidification Restrict Emiliania huxleyi Growth and Performance. Microb Ecol (2022). https://doi.org/10.1007/s00248-022-02035-3 https://hdl.handle.net/10630/24222 https://doi.org/10.1007/s00248-022-02035-3 |
op_rights |
info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/4.0/ Atribución 4.0 Internacional |
op_doi |
https://doi.org/10.1007/s00248-022-02035-3 |
container_title |
Microbial Ecology |
_version_ |
1782338261664923648 |