Genetic reassortment of influenza viruses with different origins or subtypes
In the context of A(H5N1) pandemics threat, an « avian flu and flu pandemics » project was proposed by LyonBioPole to develop influenza viruses characterization tools for vaccine production. To study genetic reassortment between influenza viruses, 3 reverse genetic systems of A(H3N2) human virus and...
Main Author: | |
---|---|
Other Authors: | , , , , , |
Format: | Doctoral or Postdoctoral Thesis |
Language: | French |
Published: |
HAL CCSD
2010
|
Subjects: | |
Online Access: | https://theses.hal.science/tel-00733681 https://theses.hal.science/tel-00733681/document https://theses.hal.science/tel-00733681/file/TH2010_Bouscambert_Duchamp_Maud.pdf |
id |
ftunivlyon:oai:HAL:tel-00733681v1 |
---|---|
record_format |
openpolar |
spelling |
ftunivlyon:oai:HAL:tel-00733681v1 2024-09-15T17:56:49+00:00 Genetic reassortment of influenza viruses with different origins or subtypes Étude du réassortiment génétique des virus influenza d’origines et de sous-types différents Bouscambert-Duchamp, Maude, Duchamp Virologie et Pathologie Humaine (VirPath) École normale supérieure de Lyon (ENS de Lyon)-Université Claude Bernard Lyon 1 (UCBL) Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS) Université Claude Bernard - Lyon I Bruno Lina Florence Morfin-Sherpa 2010-06-14 https://theses.hal.science/tel-00733681 https://theses.hal.science/tel-00733681/document https://theses.hal.science/tel-00733681/file/TH2010_Bouscambert_Duchamp_Maud.pdf fr fre HAL CCSD NNT: 2010LYO10084 tel-00733681 https://theses.hal.science/tel-00733681 https://theses.hal.science/tel-00733681/document https://theses.hal.science/tel-00733681/file/TH2010_Bouscambert_Duchamp_Maud.pdf info:eu-repo/semantics/OpenAccess https://theses.hal.science/tel-00733681 Sciences agricoles. Université Claude Bernard - Lyon I, 2010. Français. ⟨NNT : 2010LYO10084⟩ Influenza virus Genetic reassortment A(H5N1) A(H1N1)2009 Quantitative RT-PCR Reverse genetic Virus influenza Réassortiment génétique RT-PCR quantitative Génétique inverse [SDV.SA]Life Sciences [q-bio]/Agricultural sciences info:eu-repo/semantics/doctoralThesis Theses 2010 ftunivlyon 2024-07-22T23:38:55Z In the context of A(H5N1) pandemics threat, an « avian flu and flu pandemics » project was proposed by LyonBioPole to develop influenza viruses characterization tools for vaccine production. To study genetic reassortment between influenza viruses, 3 reverse genetic systems of A(H3N2) human virus and A(H5N2) and A(H5N1) avian viruses were developed and reassortant viruses were produced. Their replicative capacities were evaluated using growth kinetics on MDCK cells with viral production quantification by real-time qRT-PCR. The A(H1N1)2009 emergence raises two questions about the acquisition by genetic reassortment of oseltamivir resistance and/or pathogenicity determinants. A co-infection protocol on MDCK cells was developed to study gene constellations of reassortant viruses like A(H1N1)2009-A(H1N1) H275Y and A(H1N1)2009-A(H5N1). We report here that genetic reassortment is possible between avian, human and swine strains using reverse genetic and viral co-infection and that some specific constellations emerged. We also report, that pandemic A(H1N1)2009 can acquire the H275Y mutated NA from seasonal oseltamivir resistant A(H1N1) viruses without any modifications on replicative capacities. This genetic reassortment is also possible with A(H5N1) viruses. These observations strenght the importance of vaccination against all these influenza strains to reduce the risk of one-individual viral co-infection. Dans le contexte de la menace pandémique liée au virus influenza A(H5N1), un projet «GRIPPE AVIAIRE ET GRIPPE PANDÉMIQUE » a émergé au sein de LyonBioPôle avec comme objectif le développement d’outils de caractérisation des virus influenza pour la production de vaccins. Pour étudier le réassortiment génétique entre virus influenza, nous avons développé 3 systèmes de génétique inverse : virus humain A(H3N2) et aviaires A(H5N2) et A(H5N1) et produit des virus réassortants de composition déterminée. Leurs capacités réplicatives ont été évaluées par cinétiques de croissance virale sur MDCK avec quantification de la ... Doctoral or Postdoctoral Thesis Avian flu Université de Lyon: HAL |
institution |
Open Polar |
collection |
Université de Lyon: HAL |
op_collection_id |
ftunivlyon |
language |
French |
topic |
Influenza virus Genetic reassortment A(H5N1) A(H1N1)2009 Quantitative RT-PCR Reverse genetic Virus influenza Réassortiment génétique RT-PCR quantitative Génétique inverse [SDV.SA]Life Sciences [q-bio]/Agricultural sciences |
spellingShingle |
Influenza virus Genetic reassortment A(H5N1) A(H1N1)2009 Quantitative RT-PCR Reverse genetic Virus influenza Réassortiment génétique RT-PCR quantitative Génétique inverse [SDV.SA]Life Sciences [q-bio]/Agricultural sciences Bouscambert-Duchamp, Maude, Duchamp Genetic reassortment of influenza viruses with different origins or subtypes |
topic_facet |
Influenza virus Genetic reassortment A(H5N1) A(H1N1)2009 Quantitative RT-PCR Reverse genetic Virus influenza Réassortiment génétique RT-PCR quantitative Génétique inverse [SDV.SA]Life Sciences [q-bio]/Agricultural sciences |
description |
In the context of A(H5N1) pandemics threat, an « avian flu and flu pandemics » project was proposed by LyonBioPole to develop influenza viruses characterization tools for vaccine production. To study genetic reassortment between influenza viruses, 3 reverse genetic systems of A(H3N2) human virus and A(H5N2) and A(H5N1) avian viruses were developed and reassortant viruses were produced. Their replicative capacities were evaluated using growth kinetics on MDCK cells with viral production quantification by real-time qRT-PCR. The A(H1N1)2009 emergence raises two questions about the acquisition by genetic reassortment of oseltamivir resistance and/or pathogenicity determinants. A co-infection protocol on MDCK cells was developed to study gene constellations of reassortant viruses like A(H1N1)2009-A(H1N1) H275Y and A(H1N1)2009-A(H5N1). We report here that genetic reassortment is possible between avian, human and swine strains using reverse genetic and viral co-infection and that some specific constellations emerged. We also report, that pandemic A(H1N1)2009 can acquire the H275Y mutated NA from seasonal oseltamivir resistant A(H1N1) viruses without any modifications on replicative capacities. This genetic reassortment is also possible with A(H5N1) viruses. These observations strenght the importance of vaccination against all these influenza strains to reduce the risk of one-individual viral co-infection. Dans le contexte de la menace pandémique liée au virus influenza A(H5N1), un projet «GRIPPE AVIAIRE ET GRIPPE PANDÉMIQUE » a émergé au sein de LyonBioPôle avec comme objectif le développement d’outils de caractérisation des virus influenza pour la production de vaccins. Pour étudier le réassortiment génétique entre virus influenza, nous avons développé 3 systèmes de génétique inverse : virus humain A(H3N2) et aviaires A(H5N2) et A(H5N1) et produit des virus réassortants de composition déterminée. Leurs capacités réplicatives ont été évaluées par cinétiques de croissance virale sur MDCK avec quantification de la ... |
author2 |
Virologie et Pathologie Humaine (VirPath) École normale supérieure de Lyon (ENS de Lyon)-Université Claude Bernard Lyon 1 (UCBL) Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS) Université Claude Bernard - Lyon I Bruno Lina Florence Morfin-Sherpa |
format |
Doctoral or Postdoctoral Thesis |
author |
Bouscambert-Duchamp, Maude, Duchamp |
author_facet |
Bouscambert-Duchamp, Maude, Duchamp |
author_sort |
Bouscambert-Duchamp, Maude, Duchamp |
title |
Genetic reassortment of influenza viruses with different origins or subtypes |
title_short |
Genetic reassortment of influenza viruses with different origins or subtypes |
title_full |
Genetic reassortment of influenza viruses with different origins or subtypes |
title_fullStr |
Genetic reassortment of influenza viruses with different origins or subtypes |
title_full_unstemmed |
Genetic reassortment of influenza viruses with different origins or subtypes |
title_sort |
genetic reassortment of influenza viruses with different origins or subtypes |
publisher |
HAL CCSD |
publishDate |
2010 |
url |
https://theses.hal.science/tel-00733681 https://theses.hal.science/tel-00733681/document https://theses.hal.science/tel-00733681/file/TH2010_Bouscambert_Duchamp_Maud.pdf |
genre |
Avian flu |
genre_facet |
Avian flu |
op_source |
https://theses.hal.science/tel-00733681 Sciences agricoles. Université Claude Bernard - Lyon I, 2010. Français. ⟨NNT : 2010LYO10084⟩ |
op_relation |
NNT: 2010LYO10084 tel-00733681 https://theses.hal.science/tel-00733681 https://theses.hal.science/tel-00733681/document https://theses.hal.science/tel-00733681/file/TH2010_Bouscambert_Duchamp_Maud.pdf |
op_rights |
info:eu-repo/semantics/OpenAccess |
_version_ |
1810433010853478400 |