Molecular cloning and characterization of thyroid hormone receptors in teleost fish.
International audience Thyroid hormones are pleiotropic factors important for many developmental and physiological functions in vertebrates. Their effects are mediated by two specific receptors (TR alpha and TR beta) which are members of the nuclear hormone receptor super-family. To clarify the func...
Published in: | Journal of Molecular Endocrinology |
---|---|
Main Authors: | , , , , , |
Other Authors: | , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
HAL CCSD
2001
|
Subjects: | |
Online Access: | https://hal.science/hal-00023841 https://doi.org/10.1677/jme.0.0260051 |
id |
ftunivlyon:oai:HAL:hal-00023841v1 |
---|---|
record_format |
openpolar |
spelling |
ftunivlyon:oai:HAL:hal-00023841v1 2023-06-11T04:10:20+02:00 Molecular cloning and characterization of thyroid hormone receptors in teleost fish. Marchand, O. Safi, R. Escriva, H. van Rompaey, E. Prunet, Patrick Laudet, Vincent Laboratoire de Biologie Moléculaire de la Cellule (LBMC) École normale supérieure - Lyon (ENS Lyon)-Université Claude Bernard Lyon 1 (UCBL) Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS) MENRT, CNRS, ARC and Ligue Régionale Contre le Cancer du Rhône et de la Loire 2001 https://hal.science/hal-00023841 https://doi.org/10.1677/jme.0.0260051 en eng HAL CCSD BioScientifica info:eu-repo/semantics/altIdentifier/doi/10.1677/jme.0.0260051 hal-00023841 https://hal.science/hal-00023841 doi:10.1677/jme.0.0260051 PRODINRA: 248957 WOS: 000168115200006 ISSN: 0952-5041 EISSN: 1479-6813 Journal of Molecular Endocrinology https://hal.science/hal-00023841 Journal of Molecular Endocrinology, 2001, 26, pp.51-65. ⟨10.1677/jme.0.0260051⟩ [SDV.BA]Life Sciences [q-bio]/Animal biology info:eu-repo/semantics/article Journal articles 2001 ftunivlyon https://doi.org/10.1677/jme.0.0260051 2023-04-25T21:15:39Z International audience Thyroid hormones are pleiotropic factors important for many developmental and physiological functions in vertebrates. Their effects are mediated by two specific receptors (TR alpha and TR beta) which are members of the nuclear hormone receptor super-family. To clarify the function of these receptors, our laboratory has started a comparative study of their role in teleost fish. This type of approach has been hampered by the isolation of specific clones for each fish species studied. In this report, we describe an efficient reverse transcription/PCR procedure that allows the isolation of large fragments corresponding to TR alpha and TR beta of a wide range of teleost fish. Phylogenetic analysis of these receptors revealed a placement consistent with their origin, sequences from teleost fish being clearly monophyletic for both TR alpha and TR beta. Interestingly, this approach allowed us to isolate (from tilapia and salmon) several new TR alpha or TR beta isoforms resulting from alternative splicing. These isoforms correspond to expressed transcripts and thus may have an important physiological function. In addition, we isolated a cDNA encoding TR beta in the Atlantic salmon (Salmo salar) encoding a functional thyroid hormone receptor which binds specific thyroid hormone response elements and regulates transcription in response to thyroid hormones. Article in Journal/Newspaper Atlantic salmon Salmo salar Université de Lyon: HAL Journal of Molecular Endocrinology 26 1 51 65 |
institution |
Open Polar |
collection |
Université de Lyon: HAL |
op_collection_id |
ftunivlyon |
language |
English |
topic |
[SDV.BA]Life Sciences [q-bio]/Animal biology |
spellingShingle |
[SDV.BA]Life Sciences [q-bio]/Animal biology Marchand, O. Safi, R. Escriva, H. van Rompaey, E. Prunet, Patrick Laudet, Vincent Molecular cloning and characterization of thyroid hormone receptors in teleost fish. |
topic_facet |
[SDV.BA]Life Sciences [q-bio]/Animal biology |
description |
International audience Thyroid hormones are pleiotropic factors important for many developmental and physiological functions in vertebrates. Their effects are mediated by two specific receptors (TR alpha and TR beta) which are members of the nuclear hormone receptor super-family. To clarify the function of these receptors, our laboratory has started a comparative study of their role in teleost fish. This type of approach has been hampered by the isolation of specific clones for each fish species studied. In this report, we describe an efficient reverse transcription/PCR procedure that allows the isolation of large fragments corresponding to TR alpha and TR beta of a wide range of teleost fish. Phylogenetic analysis of these receptors revealed a placement consistent with their origin, sequences from teleost fish being clearly monophyletic for both TR alpha and TR beta. Interestingly, this approach allowed us to isolate (from tilapia and salmon) several new TR alpha or TR beta isoforms resulting from alternative splicing. These isoforms correspond to expressed transcripts and thus may have an important physiological function. In addition, we isolated a cDNA encoding TR beta in the Atlantic salmon (Salmo salar) encoding a functional thyroid hormone receptor which binds specific thyroid hormone response elements and regulates transcription in response to thyroid hormones. |
author2 |
Laboratoire de Biologie Moléculaire de la Cellule (LBMC) École normale supérieure - Lyon (ENS Lyon)-Université Claude Bernard Lyon 1 (UCBL) Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS) MENRT, CNRS, ARC and Ligue Régionale Contre le Cancer du Rhône et de la Loire |
format |
Article in Journal/Newspaper |
author |
Marchand, O. Safi, R. Escriva, H. van Rompaey, E. Prunet, Patrick Laudet, Vincent |
author_facet |
Marchand, O. Safi, R. Escriva, H. van Rompaey, E. Prunet, Patrick Laudet, Vincent |
author_sort |
Marchand, O. |
title |
Molecular cloning and characterization of thyroid hormone receptors in teleost fish. |
title_short |
Molecular cloning and characterization of thyroid hormone receptors in teleost fish. |
title_full |
Molecular cloning and characterization of thyroid hormone receptors in teleost fish. |
title_fullStr |
Molecular cloning and characterization of thyroid hormone receptors in teleost fish. |
title_full_unstemmed |
Molecular cloning and characterization of thyroid hormone receptors in teleost fish. |
title_sort |
molecular cloning and characterization of thyroid hormone receptors in teleost fish. |
publisher |
HAL CCSD |
publishDate |
2001 |
url |
https://hal.science/hal-00023841 https://doi.org/10.1677/jme.0.0260051 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
ISSN: 0952-5041 EISSN: 1479-6813 Journal of Molecular Endocrinology https://hal.science/hal-00023841 Journal of Molecular Endocrinology, 2001, 26, pp.51-65. ⟨10.1677/jme.0.0260051⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1677/jme.0.0260051 hal-00023841 https://hal.science/hal-00023841 doi:10.1677/jme.0.0260051 PRODINRA: 248957 WOS: 000168115200006 |
op_doi |
https://doi.org/10.1677/jme.0.0260051 |
container_title |
Journal of Molecular Endocrinology |
container_volume |
26 |
container_issue |
1 |
container_start_page |
51 |
op_container_end_page |
65 |
_version_ |
1768384674851192832 |