Potential implication of mitochondrial fusion proteins in the ontogeny of muscle bioenergetics in birds
Cold-exposed young birds maintain their homeothermy by stimulating mitochondrial oxidations in skeletal muscle. Prolonged cold exposure enhances muscle thermogenic capacities through mitochondrial bioenergetics plasticity which control still remains hypothetical. In mammals, fusion proteins (mitofus...
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Online Access: | https://theses.hal.science/tel-01127600 https://theses.hal.science/tel-01127600/document https://theses.hal.science/tel-01127600/file/2013LYO10276.pdf |
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ftunivlyon1:oai:HAL:tel-01127600v1 2024-05-12T07:54:44+00:00 Potential implication of mitochondrial fusion proteins in the ontogeny of muscle bioenergetics in birds Implication potentielle des protéines de fusion mitochondriale dans l'ontogenèse des processus bioénergétiques musculaires chez l'oiseau Fongy, Anaïs Physiologie intégrative, cellulaire et moléculaire (PICM) Université Claude Bernard Lyon 1 (UCBL) Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS) Laboratoire d'Ecologie des Hydrosystèmes Naturels et Anthropisés (LEHNA) Université de Lyon-Université de Lyon-École Nationale des Travaux Publics de l'État (ENTPE)-Centre National de la Recherche Scientifique (CNRS) Université Claude Bernard - Lyon I Claude Duchamp Caroline Romestaing 2013-11-26 https://theses.hal.science/tel-01127600 https://theses.hal.science/tel-01127600/document https://theses.hal.science/tel-01127600/file/2013LYO10276.pdf fr fre HAL CCSD NNT: 2013LYO10276 tel-01127600 https://theses.hal.science/tel-01127600 https://theses.hal.science/tel-01127600/document https://theses.hal.science/tel-01127600/file/2013LYO10276.pdf info:eu-repo/semantics/OpenAccess https://theses.hal.science/tel-01127600 Sciences agricoles. Université Claude Bernard - Lyon I, 2013. Français. ⟨NNT : 2013LYO10276⟩ Adélie penguins Ducklings Mitochondrial activity Permeabilized fibers OPA1 Mfn2 Energetic constraints Thyroid hormones Birds Manchots Adélie Canetons Bioénergétique mitochondriale Fibres perméabilisées Contraintes énergétiques Hormones thyroïdiennes Oiseaux [SDV.SA]Life Sciences [q-bio]/Agricultural sciences info:eu-repo/semantics/doctoralThesis Theses 2013 ftunivlyon1 2024-04-18T01:51:05Z Cold-exposed young birds maintain their homeothermy by stimulating mitochondrial oxidations in skeletal muscle. Prolonged cold exposure enhances muscle thermogenic capacities through mitochondrial bioenergetics plasticity which control still remains hypothetical. In mammals, fusion proteins (mitofusins (Mfns) and OPA1 (Optic Atrophy 1)) contribute to the permanent and dynamic changes in mitochondrial networks in multiple cell types. The aim of our work was to characterize the expression of avian homologues of mammalian fusion proteins and to study the variations of their expression during the establishment of bioenergetics processes in growing birds, during an acute or a prolonged cold exposure and finally during nutritional or endocrine challenges. Methodologically, an integrative approach has been used from whole animal (indirect calorimetry) to protein (western-blot) or gene (RT-PCR) expression through measurements of the bioenergetics functionality of permeabilized muscle fibers and isolated mitochondria. Two animal models were used, a species naturally adapted to Antarctica harsh conditions, the Adélie penguin (Pygoscelis adeliae), and a laboratory model, the Muscovy duck (Cairina moschata).Our results allowed us to characterize, in birds, the expression of immunoreactive fusion proteins (Mfn2, OPA1) which were homologous to those of mammals. The sequencing of a part of the coding sequence of Mfns genes showed a great similitude between avian and mammalian species. In penguins, the relative abundance of these proteins in muscle mitochondria was modified by growth in the cold and was positively correlated with muscle bioenergetics capacities. In ducks, the respiratory activity and the relative abundance of these proteins were also correlated after a 60h fasting period or,though a lesser extent, after a pharmacological alteration of thyroid status. Our results show, for the first time in birds, the expression of proteins homologous to mammalian fusion proteins. The association between the changes in ... Doctoral or Postdoctoral Thesis Antarc* Antarctica Pygoscelis adeliae HAL Lyon 1 (University Claude Bernard Lyon 1) Manchots ENVELOPE(141.397,141.397,-66.811,-66.811) |
institution |
Open Polar |
collection |
HAL Lyon 1 (University Claude Bernard Lyon 1) |
op_collection_id |
ftunivlyon1 |
language |
French |
topic |
Adélie penguins Ducklings Mitochondrial activity Permeabilized fibers OPA1 Mfn2 Energetic constraints Thyroid hormones Birds Manchots Adélie Canetons Bioénergétique mitochondriale Fibres perméabilisées Contraintes énergétiques Hormones thyroïdiennes Oiseaux [SDV.SA]Life Sciences [q-bio]/Agricultural sciences |
spellingShingle |
Adélie penguins Ducklings Mitochondrial activity Permeabilized fibers OPA1 Mfn2 Energetic constraints Thyroid hormones Birds Manchots Adélie Canetons Bioénergétique mitochondriale Fibres perméabilisées Contraintes énergétiques Hormones thyroïdiennes Oiseaux [SDV.SA]Life Sciences [q-bio]/Agricultural sciences Fongy, Anaïs Potential implication of mitochondrial fusion proteins in the ontogeny of muscle bioenergetics in birds |
topic_facet |
Adélie penguins Ducklings Mitochondrial activity Permeabilized fibers OPA1 Mfn2 Energetic constraints Thyroid hormones Birds Manchots Adélie Canetons Bioénergétique mitochondriale Fibres perméabilisées Contraintes énergétiques Hormones thyroïdiennes Oiseaux [SDV.SA]Life Sciences [q-bio]/Agricultural sciences |
description |
Cold-exposed young birds maintain their homeothermy by stimulating mitochondrial oxidations in skeletal muscle. Prolonged cold exposure enhances muscle thermogenic capacities through mitochondrial bioenergetics plasticity which control still remains hypothetical. In mammals, fusion proteins (mitofusins (Mfns) and OPA1 (Optic Atrophy 1)) contribute to the permanent and dynamic changes in mitochondrial networks in multiple cell types. The aim of our work was to characterize the expression of avian homologues of mammalian fusion proteins and to study the variations of their expression during the establishment of bioenergetics processes in growing birds, during an acute or a prolonged cold exposure and finally during nutritional or endocrine challenges. Methodologically, an integrative approach has been used from whole animal (indirect calorimetry) to protein (western-blot) or gene (RT-PCR) expression through measurements of the bioenergetics functionality of permeabilized muscle fibers and isolated mitochondria. Two animal models were used, a species naturally adapted to Antarctica harsh conditions, the Adélie penguin (Pygoscelis adeliae), and a laboratory model, the Muscovy duck (Cairina moschata).Our results allowed us to characterize, in birds, the expression of immunoreactive fusion proteins (Mfn2, OPA1) which were homologous to those of mammals. The sequencing of a part of the coding sequence of Mfns genes showed a great similitude between avian and mammalian species. In penguins, the relative abundance of these proteins in muscle mitochondria was modified by growth in the cold and was positively correlated with muscle bioenergetics capacities. In ducks, the respiratory activity and the relative abundance of these proteins were also correlated after a 60h fasting period or,though a lesser extent, after a pharmacological alteration of thyroid status. Our results show, for the first time in birds, the expression of proteins homologous to mammalian fusion proteins. The association between the changes in ... |
author2 |
Physiologie intégrative, cellulaire et moléculaire (PICM) Université Claude Bernard Lyon 1 (UCBL) Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS) Laboratoire d'Ecologie des Hydrosystèmes Naturels et Anthropisés (LEHNA) Université de Lyon-Université de Lyon-École Nationale des Travaux Publics de l'État (ENTPE)-Centre National de la Recherche Scientifique (CNRS) Université Claude Bernard - Lyon I Claude Duchamp Caroline Romestaing |
format |
Doctoral or Postdoctoral Thesis |
author |
Fongy, Anaïs |
author_facet |
Fongy, Anaïs |
author_sort |
Fongy, Anaïs |
title |
Potential implication of mitochondrial fusion proteins in the ontogeny of muscle bioenergetics in birds |
title_short |
Potential implication of mitochondrial fusion proteins in the ontogeny of muscle bioenergetics in birds |
title_full |
Potential implication of mitochondrial fusion proteins in the ontogeny of muscle bioenergetics in birds |
title_fullStr |
Potential implication of mitochondrial fusion proteins in the ontogeny of muscle bioenergetics in birds |
title_full_unstemmed |
Potential implication of mitochondrial fusion proteins in the ontogeny of muscle bioenergetics in birds |
title_sort |
potential implication of mitochondrial fusion proteins in the ontogeny of muscle bioenergetics in birds |
publisher |
HAL CCSD |
publishDate |
2013 |
url |
https://theses.hal.science/tel-01127600 https://theses.hal.science/tel-01127600/document https://theses.hal.science/tel-01127600/file/2013LYO10276.pdf |
long_lat |
ENVELOPE(141.397,141.397,-66.811,-66.811) |
geographic |
Manchots |
geographic_facet |
Manchots |
genre |
Antarc* Antarctica Pygoscelis adeliae |
genre_facet |
Antarc* Antarctica Pygoscelis adeliae |
op_source |
https://theses.hal.science/tel-01127600 Sciences agricoles. Université Claude Bernard - Lyon I, 2013. Français. ⟨NNT : 2013LYO10276⟩ |
op_relation |
NNT: 2013LYO10276 tel-01127600 https://theses.hal.science/tel-01127600 https://theses.hal.science/tel-01127600/document https://theses.hal.science/tel-01127600/file/2013LYO10276.pdf |
op_rights |
info:eu-repo/semantics/OpenAccess |
_version_ |
1798851746192162816 |