Etude de la toxicité des micropolluants organochlorés chez le poisson de profondeur Coryphaenoides rupestris : utilisation des tranches de précision de foie
Deep-sea fish are highly contaminated with organochlorine compounds(OCs), which are persistent man-made chemicals. Because it is very difficult to maintain these fish alive for toxicological experiments, the real impact of such contamination remains unknown. By analysing liver samples of the deep-se...
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ftunivlouvain:oai:dial.uclouvain.be:boreal:85991 2024-05-19T07:47:57+00:00 Etude de la toxicité des micropolluants organochlorés chez le poisson de profondeur Coryphaenoides rupestris : utilisation des tranches de précision de foie Lemaire, Benjamin UCL - SST/ISV/ISV - Institut des sciences de la vie Rees, Jean-François Van Den Bosch, Philippe Debier, Cathy Buc Calderon, Pedro Thome, Jean-Pierre Sebert, Philippe 2011 http://hdl.handle.net/2078.1/85991 fre fre boreal:85991 http://hdl.handle.net/2078.1/85991 info:eu-repo/semantics/restrictedAccess Cytochrome P4501A Coryphaenoides rupestris Hydrostatic pressure Oxidative stress Precision-cut liver slices info:eu-repo/semantics/doctoralThesis 2011 ftunivlouvain 2024-04-24T01:44:30Z Deep-sea fish are highly contaminated with organochlorine compounds(OCs), which are persistent man-made chemicals. Because it is very difficult to maintain these fish alive for toxicological experiments, the real impact of such contamination remains unknown. By analysing liver samples of the deep-sea grenadier Coryphaenoides rupestris (200 - 2600 meter depth), we first observed signs of an oxidative impact linked to OCs' metabolism. The activity of the phase 1 detoxification cytochrome P4501A (CYP1A) enzyme was positively correlated to OCs loads and antioxidant enzymes activities (catalase, superoxide dismutase). Still, the magnitude of CYP1A induction was fairly low compared to the huge contamination. We then developed the precision-cut liver slices (PCLS) technology to study more in-depth the oxidative impact of OCs' metabolism on living liver cells of C. rupestris. Two shallow fish (Salmo salar, Dicentrarchus labrax) were also chosen as basis for comparison. Hyperbaric cylinders were used to investigate in this comparative study the effects of the high hydrostatic pressure (typical of the deep-sea) on the response of liver cells of these fish to contaminants, their pro- and anti-oxidant status and that of heat shock proteins (also known to participate in a general stress response to toxicants). Apart from the obtention of the first-ever data on living deep-sea fish liver cells, we found a striking pressure-dependent reduction of CYP1A induction in each species, suggesting that deep-sea fish might be less prone to the oxidative impact of OCs metabolism in their environment (a feature that might explain their high OCs loads). (BIOL 3) -- UCL, 2011 Doctoral or Postdoctoral Thesis Salmo salar DIAL@UCLouvain (Université catholique de Louvain) |
institution |
Open Polar |
collection |
DIAL@UCLouvain (Université catholique de Louvain) |
op_collection_id |
ftunivlouvain |
language |
French |
topic |
Cytochrome P4501A Coryphaenoides rupestris Hydrostatic pressure Oxidative stress Precision-cut liver slices |
spellingShingle |
Cytochrome P4501A Coryphaenoides rupestris Hydrostatic pressure Oxidative stress Precision-cut liver slices Lemaire, Benjamin Etude de la toxicité des micropolluants organochlorés chez le poisson de profondeur Coryphaenoides rupestris : utilisation des tranches de précision de foie |
topic_facet |
Cytochrome P4501A Coryphaenoides rupestris Hydrostatic pressure Oxidative stress Precision-cut liver slices |
description |
Deep-sea fish are highly contaminated with organochlorine compounds(OCs), which are persistent man-made chemicals. Because it is very difficult to maintain these fish alive for toxicological experiments, the real impact of such contamination remains unknown. By analysing liver samples of the deep-sea grenadier Coryphaenoides rupestris (200 - 2600 meter depth), we first observed signs of an oxidative impact linked to OCs' metabolism. The activity of the phase 1 detoxification cytochrome P4501A (CYP1A) enzyme was positively correlated to OCs loads and antioxidant enzymes activities (catalase, superoxide dismutase). Still, the magnitude of CYP1A induction was fairly low compared to the huge contamination. We then developed the precision-cut liver slices (PCLS) technology to study more in-depth the oxidative impact of OCs' metabolism on living liver cells of C. rupestris. Two shallow fish (Salmo salar, Dicentrarchus labrax) were also chosen as basis for comparison. Hyperbaric cylinders were used to investigate in this comparative study the effects of the high hydrostatic pressure (typical of the deep-sea) on the response of liver cells of these fish to contaminants, their pro- and anti-oxidant status and that of heat shock proteins (also known to participate in a general stress response to toxicants). Apart from the obtention of the first-ever data on living deep-sea fish liver cells, we found a striking pressure-dependent reduction of CYP1A induction in each species, suggesting that deep-sea fish might be less prone to the oxidative impact of OCs metabolism in their environment (a feature that might explain their high OCs loads). (BIOL 3) -- UCL, 2011 |
author2 |
UCL - SST/ISV/ISV - Institut des sciences de la vie Rees, Jean-François Van Den Bosch, Philippe Debier, Cathy Buc Calderon, Pedro Thome, Jean-Pierre Sebert, Philippe |
format |
Doctoral or Postdoctoral Thesis |
author |
Lemaire, Benjamin |
author_facet |
Lemaire, Benjamin |
author_sort |
Lemaire, Benjamin |
title |
Etude de la toxicité des micropolluants organochlorés chez le poisson de profondeur Coryphaenoides rupestris : utilisation des tranches de précision de foie |
title_short |
Etude de la toxicité des micropolluants organochlorés chez le poisson de profondeur Coryphaenoides rupestris : utilisation des tranches de précision de foie |
title_full |
Etude de la toxicité des micropolluants organochlorés chez le poisson de profondeur Coryphaenoides rupestris : utilisation des tranches de précision de foie |
title_fullStr |
Etude de la toxicité des micropolluants organochlorés chez le poisson de profondeur Coryphaenoides rupestris : utilisation des tranches de précision de foie |
title_full_unstemmed |
Etude de la toxicité des micropolluants organochlorés chez le poisson de profondeur Coryphaenoides rupestris : utilisation des tranches de précision de foie |
title_sort |
etude de la toxicitã© des micropolluants organochlorã©s chez le poisson de profondeur coryphaenoides rupestris : utilisation des tranches de prã©cision de foie |
publishDate |
2011 |
url |
http://hdl.handle.net/2078.1/85991 |
genre |
Salmo salar |
genre_facet |
Salmo salar |
op_relation |
boreal:85991 http://hdl.handle.net/2078.1/85991 |
op_rights |
info:eu-repo/semantics/restrictedAccess |
_version_ |
1799488448996835328 |