pGIAK1, a Heavy Metal Resistant Plasmid from an Obligate Alkaliphilic and Halotolerant Bacterium Isolated from the Antarctic Concordia Station Confined Environment
pGIAK1 is a 38-kb plasmid originating from the obligate alkaliphilic and halotolerant Bacillaceae strain JMAK1. The strain was originally isolated from the confined environments of the Antarctic Concordia station. Analysis of the pGIAK1 38,362-bp sequence revealed that, in addition to its replicatio...
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ftunivlouvain:oai:dial.uclouvain.be:boreal:140691 2024-05-12T07:56:39+00:00 pGIAK1, a Heavy Metal Resistant Plasmid from an Obligate Alkaliphilic and Halotolerant Bacterium Isolated from the Antarctic Concordia Station Confined Environment Guo, Suxia Mahillon, Jacques UCL - SST/ELI/ELIM - Applied Microbiology 2013 http://hdl.handle.net/2078.1/140691 https://doi.org/10.1371/journal.pone.0072461 ng ndo Public Library of Science boreal:140691 http://hdl.handle.net/2078.1/140691 doi:10.1371/journal.pone.0072461 urn:ISSN:1932-6203 urn:EISSN:1932-6203 info:eu-repo/semantics/openAccess PLoS One, Vol. 8, no.8, p. e72461 (2013) info:eu-repo/semantics/article 2013 ftunivlouvain https://doi.org/10.1371/journal.pone.0072461 2024-04-17T17:17:42Z pGIAK1 is a 38-kb plasmid originating from the obligate alkaliphilic and halotolerant Bacillaceae strain JMAK1. The strain was originally isolated from the confined environments of the Antarctic Concordia station. Analysis of the pGIAK1 38,362-bp sequence revealed that, in addition to its replication region, this plasmid contains the genetic determinants for cadmium and arsenic resistances, putative methyltransferase, tyrosine recombinase, spore coat protein and potassium transport protein, as well as several hypothetical proteins. Cloning the pGIAK1 cad operon in Bacillus cereus H3081.97 and its ars operon in Bacillus subtilis 1A280 conferred to these hosts cadmium and arsenic resistances, respectively, therefore confirming their bona fide activities. The pGIAK1 replicon region was also shown to be functional in Bacillus thuringiensis, Bacillus subtilis and Staphylococcus aureus, but was only stably maintained in B. subtilis. Finally, using an Escherichia coli - B. thuringiensis shuttle BAC vector, pGIAK1 was shown to display conjugative properties since it was able to transfer the BAC plasmid among B. thuringiensis strains. © 2013 Guo, Mahillon. Article in Journal/Newspaper Antarc* Antarctic DIAL@UCLouvain (Université catholique de Louvain) Antarctic Concordia Station ENVELOPE(123.333,123.333,-75.100,-75.100) The Antarctic PLoS ONE 8 8 e72461 |
institution |
Open Polar |
collection |
DIAL@UCLouvain (Université catholique de Louvain) |
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ftunivlouvain |
language |
Ndonga |
description |
pGIAK1 is a 38-kb plasmid originating from the obligate alkaliphilic and halotolerant Bacillaceae strain JMAK1. The strain was originally isolated from the confined environments of the Antarctic Concordia station. Analysis of the pGIAK1 38,362-bp sequence revealed that, in addition to its replication region, this plasmid contains the genetic determinants for cadmium and arsenic resistances, putative methyltransferase, tyrosine recombinase, spore coat protein and potassium transport protein, as well as several hypothetical proteins. Cloning the pGIAK1 cad operon in Bacillus cereus H3081.97 and its ars operon in Bacillus subtilis 1A280 conferred to these hosts cadmium and arsenic resistances, respectively, therefore confirming their bona fide activities. The pGIAK1 replicon region was also shown to be functional in Bacillus thuringiensis, Bacillus subtilis and Staphylococcus aureus, but was only stably maintained in B. subtilis. Finally, using an Escherichia coli - B. thuringiensis shuttle BAC vector, pGIAK1 was shown to display conjugative properties since it was able to transfer the BAC plasmid among B. thuringiensis strains. © 2013 Guo, Mahillon. |
author2 |
UCL - SST/ELI/ELIM - Applied Microbiology |
format |
Article in Journal/Newspaper |
author |
Guo, Suxia Mahillon, Jacques |
spellingShingle |
Guo, Suxia Mahillon, Jacques pGIAK1, a Heavy Metal Resistant Plasmid from an Obligate Alkaliphilic and Halotolerant Bacterium Isolated from the Antarctic Concordia Station Confined Environment |
author_facet |
Guo, Suxia Mahillon, Jacques |
author_sort |
Guo, Suxia |
title |
pGIAK1, a Heavy Metal Resistant Plasmid from an Obligate Alkaliphilic and Halotolerant Bacterium Isolated from the Antarctic Concordia Station Confined Environment |
title_short |
pGIAK1, a Heavy Metal Resistant Plasmid from an Obligate Alkaliphilic and Halotolerant Bacterium Isolated from the Antarctic Concordia Station Confined Environment |
title_full |
pGIAK1, a Heavy Metal Resistant Plasmid from an Obligate Alkaliphilic and Halotolerant Bacterium Isolated from the Antarctic Concordia Station Confined Environment |
title_fullStr |
pGIAK1, a Heavy Metal Resistant Plasmid from an Obligate Alkaliphilic and Halotolerant Bacterium Isolated from the Antarctic Concordia Station Confined Environment |
title_full_unstemmed |
pGIAK1, a Heavy Metal Resistant Plasmid from an Obligate Alkaliphilic and Halotolerant Bacterium Isolated from the Antarctic Concordia Station Confined Environment |
title_sort |
pgiak1, a heavy metal resistant plasmid from an obligate alkaliphilic and halotolerant bacterium isolated from the antarctic concordia station confined environment |
publisher |
Public Library of Science |
publishDate |
2013 |
url |
http://hdl.handle.net/2078.1/140691 https://doi.org/10.1371/journal.pone.0072461 |
long_lat |
ENVELOPE(123.333,123.333,-75.100,-75.100) |
geographic |
Antarctic Concordia Station The Antarctic |
geographic_facet |
Antarctic Concordia Station The Antarctic |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_source |
PLoS One, Vol. 8, no.8, p. e72461 (2013) |
op_relation |
boreal:140691 http://hdl.handle.net/2078.1/140691 doi:10.1371/journal.pone.0072461 urn:ISSN:1932-6203 urn:EISSN:1932-6203 |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1371/journal.pone.0072461 |
container_title |
PLoS ONE |
container_volume |
8 |
container_issue |
8 |
container_start_page |
e72461 |
_version_ |
1798836833480605696 |