Relationships between in vitro lymphoproliferative responses and levels of contaminants in blood of free-ranging adult harbour seals (Phoca vitulina) from the North Sea
In vitro culture of peripheral blood leucocytes (PBLs) is currently used in toxicological studies of marine mammals. However, blood cells of wild individuals are exposed in vivo to environmental contaminants before being isolated and exposed to contaminants in vitro. The aim of this study was to hig...
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Online Access: | http://hdl.handle.net/2078.1/134966 https://doi.org/10.1016/j.aquatox.2013.08.014 |
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ftunivlouvain:oai:dial.uclouvain.be:boreal:134966 2024-05-19T07:47:20+00:00 Relationships between in vitro lymphoproliferative responses and levels of contaminants in blood of free-ranging adult harbour seals (Phoca vitulina) from the North Sea Dupont, Aurélie Siebert, Ursula Covaci, Adrien Weijs, Lisbeth Eppe , Gauthier Debier, Cathy De pauw-gillet, Marie-Claire Das, Krishna UCL - SST/ISV - Institut des sciences de la vie 2013 http://hdl.handle.net/2078.1/134966 https://doi.org/10.1016/j.aquatox.2013.08.014 eng eng Elsevier BV boreal:134966 http://hdl.handle.net/2078.1/134966 doi:10.1016/j.aquatox.2013.08.014 urn:ISSN:0166-445X urn:EISSN:1879-1514 info:eu-repo/semantics/openAccess Aquatic Toxicology, Vol. 142-143, p. 210-220 (2013) Phoca vitulina Blood Contaminant In vitro Lymphoproliferation Immune parameters info:eu-repo/semantics/article 2013 ftunivlouvain https://doi.org/10.1016/j.aquatox.2013.08.014 2024-04-24T01:37:34Z In vitro culture of peripheral blood leucocytes (PBLs) is currently used in toxicological studies of marine mammals. However, blood cells of wild individuals are exposed in vivo to environmental contaminants before being isolated and exposed to contaminants in vitro. The aim of this study was to highlight potential relationships between blood contaminant levels and in vitro peripheral blood lymphocyte proliferation in free-ranging adult harbour seals (Phoca vitulina) from the North Sea. Blood samples of 18 individuals were analyzed for trace elements (Fe, Zn, Se, Cu, Hg, Pb, Cd) and persistent organic contaminants and metabolites (σPCBs, σHO-PCBs, σPBDEs, 2-MeO-BDE68 and 6-MeO-BDE47, σDDXs, hexachlorobenzene, oxychlordane, trans-nonachlor, pentachlorophenol and tribromoanisole). The same samples were used to determine the haematology profiles, cell numbers and viability, as well as the in vitro ConA-induced lymphocyte proliferation expressed as a stimulation index (SI). Correlation tests (Bravais-Pearson) and Principal Component Analysis with multiple regression revealed no statistically significant relationship between the lymphocyte SI and the contaminants studied. However, the number of lymphocytes per millilitre of whole blood appeared to be negatively correlated to pentachlorophenol (r= -0.63, p= 0.005). In adult harbour seals, the interindividual variations of in vitro lymphocyte proliferation did not appear to be directly linked to pollutant levels present in the blood, and it is likely that other factors such as age, life history, or physiological parameters have an influence. In a general manner, experiments with in vitro immune cell cultures of wild marine mammals should be designed so as to minimize confounding factors in which case they remain a valuable tool to study pollutant effects in vitro. © 2013 Elsevier B.V. Article in Journal/Newspaper Phoca vitulina DIAL@UCLouvain (Université catholique de Louvain) Aquatic Toxicology 142-143 210 220 |
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Open Polar |
collection |
DIAL@UCLouvain (Université catholique de Louvain) |
op_collection_id |
ftunivlouvain |
language |
English |
topic |
Phoca vitulina Blood Contaminant In vitro Lymphoproliferation Immune parameters |
spellingShingle |
Phoca vitulina Blood Contaminant In vitro Lymphoproliferation Immune parameters Dupont, Aurélie Siebert, Ursula Covaci, Adrien Weijs, Lisbeth Eppe , Gauthier Debier, Cathy De pauw-gillet, Marie-Claire Das, Krishna Relationships between in vitro lymphoproliferative responses and levels of contaminants in blood of free-ranging adult harbour seals (Phoca vitulina) from the North Sea |
topic_facet |
Phoca vitulina Blood Contaminant In vitro Lymphoproliferation Immune parameters |
description |
In vitro culture of peripheral blood leucocytes (PBLs) is currently used in toxicological studies of marine mammals. However, blood cells of wild individuals are exposed in vivo to environmental contaminants before being isolated and exposed to contaminants in vitro. The aim of this study was to highlight potential relationships between blood contaminant levels and in vitro peripheral blood lymphocyte proliferation in free-ranging adult harbour seals (Phoca vitulina) from the North Sea. Blood samples of 18 individuals were analyzed for trace elements (Fe, Zn, Se, Cu, Hg, Pb, Cd) and persistent organic contaminants and metabolites (σPCBs, σHO-PCBs, σPBDEs, 2-MeO-BDE68 and 6-MeO-BDE47, σDDXs, hexachlorobenzene, oxychlordane, trans-nonachlor, pentachlorophenol and tribromoanisole). The same samples were used to determine the haematology profiles, cell numbers and viability, as well as the in vitro ConA-induced lymphocyte proliferation expressed as a stimulation index (SI). Correlation tests (Bravais-Pearson) and Principal Component Analysis with multiple regression revealed no statistically significant relationship between the lymphocyte SI and the contaminants studied. However, the number of lymphocytes per millilitre of whole blood appeared to be negatively correlated to pentachlorophenol (r= -0.63, p= 0.005). In adult harbour seals, the interindividual variations of in vitro lymphocyte proliferation did not appear to be directly linked to pollutant levels present in the blood, and it is likely that other factors such as age, life history, or physiological parameters have an influence. In a general manner, experiments with in vitro immune cell cultures of wild marine mammals should be designed so as to minimize confounding factors in which case they remain a valuable tool to study pollutant effects in vitro. © 2013 Elsevier B.V. |
author2 |
UCL - SST/ISV - Institut des sciences de la vie |
format |
Article in Journal/Newspaper |
author |
Dupont, Aurélie Siebert, Ursula Covaci, Adrien Weijs, Lisbeth Eppe , Gauthier Debier, Cathy De pauw-gillet, Marie-Claire Das, Krishna |
author_facet |
Dupont, Aurélie Siebert, Ursula Covaci, Adrien Weijs, Lisbeth Eppe , Gauthier Debier, Cathy De pauw-gillet, Marie-Claire Das, Krishna |
author_sort |
Dupont, Aurélie |
title |
Relationships between in vitro lymphoproliferative responses and levels of contaminants in blood of free-ranging adult harbour seals (Phoca vitulina) from the North Sea |
title_short |
Relationships between in vitro lymphoproliferative responses and levels of contaminants in blood of free-ranging adult harbour seals (Phoca vitulina) from the North Sea |
title_full |
Relationships between in vitro lymphoproliferative responses and levels of contaminants in blood of free-ranging adult harbour seals (Phoca vitulina) from the North Sea |
title_fullStr |
Relationships between in vitro lymphoproliferative responses and levels of contaminants in blood of free-ranging adult harbour seals (Phoca vitulina) from the North Sea |
title_full_unstemmed |
Relationships between in vitro lymphoproliferative responses and levels of contaminants in blood of free-ranging adult harbour seals (Phoca vitulina) from the North Sea |
title_sort |
relationships between in vitro lymphoproliferative responses and levels of contaminants in blood of free-ranging adult harbour seals (phoca vitulina) from the north sea |
publisher |
Elsevier BV |
publishDate |
2013 |
url |
http://hdl.handle.net/2078.1/134966 https://doi.org/10.1016/j.aquatox.2013.08.014 |
genre |
Phoca vitulina |
genre_facet |
Phoca vitulina |
op_source |
Aquatic Toxicology, Vol. 142-143, p. 210-220 (2013) |
op_relation |
boreal:134966 http://hdl.handle.net/2078.1/134966 doi:10.1016/j.aquatox.2013.08.014 urn:ISSN:0166-445X urn:EISSN:1879-1514 |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1016/j.aquatox.2013.08.014 |
container_title |
Aquatic Toxicology |
container_volume |
142-143 |
container_start_page |
210 |
op_container_end_page |
220 |
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1799487732429357056 |