Gene expression changes governing extreme dehydration tolerance in an Antarctic insect
Among terrestrial organisms, arthropods are especially susceptible to dehydration, given their small body size and high surface area to volume ratio. This challenge is particularly acute for polar arthropods that face near-constant desiccating conditions, as water is frozen and thus unavailable for...
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Online Access: | http://hdl.handle.net/2078.1/121667 https://doi.org/10.1073/pnas.1218661109 |
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ftunivlouvain:oai:dial.uclouvain.be:boreal:121667 2024-05-19T07:31:07+00:00 Gene expression changes governing extreme dehydration tolerance in an Antarctic insect Teets, Nicholas M. Peyton, Justin T. Colinet, Hervé Renault, David Kelley, Joanna L. Kawarasaki, Yuta Lee, Richard E. Denlinger, David L. UCL - SST/ELI/ELIB - Biodiversity 2012 http://hdl.handle.net/2078.1/121667 https://doi.org/10.1073/pnas.1218661109 eng eng National academy of sciences boreal:121667 http://hdl.handle.net/2078.1/121667 doi:10.1073/pnas.1218661109 urn:ISSN:0027-8424 urn:EISSN:1091-6490 info:eu-repo/semantics/restrictedAccess Proceedings of the National academy of sciences of the United States of America, Vol. 109, no.50, p. 20744-20749 (2012) 3123 QH301 info:eu-repo/semantics/article 2012 ftunivlouvain https://doi.org/10.1073/pnas.1218661109 2024-04-24T01:39:46Z Among terrestrial organisms, arthropods are especially susceptible to dehydration, given their small body size and high surface area to volume ratio. This challenge is particularly acute for polar arthropods that face near-constant desiccating conditions, as water is frozen and thus unavailable for much of the year. The molecular mechanisms that govern extreme dehydration tolerance in insects remain largely undefined. In this study, we used RNA sequencing to quantify transcriptional mechanisms of extreme dehydration tolerance in the Antarctic midge, Belgica antarctica, the world's southernmost insect and only insect endemic to Antarctica. Larvae of B. antarctica are remarkably tolerant of dehydration, surviving losses up to 70% of their body water. Gene expression changes in response to dehydration indicated up-regulation of cellular recycling pathways including the ubiquitin-mediated proteasome and autophagy, with concurrent down-regulation of genes involved in general metabolism and ATP production. Metabolomics results revealed shifts in metabolite pools that correlated closely with changes in gene expression, indicating that coordinated changes in gene expression and metabolism are a critical component of the dehydration response. Finally, using comparative genomics, we compared our gene expression results with a transcriptomic dataset for the Arctic collembolan, Megaphorura arctica. Although B. antarctica and M. arctica are adapted to similar environments, our analysis indicated very little overlap in expression profiles between these two arthropods. Whereas several orthologous genes showed similar expression patterns, transcriptional changes were largely species specific, indicating these polar arthropods have developed distinct transcriptional mechanisms to cope with similar desiccating conditions. Article in Journal/Newspaper Antarc* Antarctic Antarctic midge Antarctica Arctic Belgica antarctica DIAL@UCLouvain (Université catholique de Louvain) Proceedings of the National Academy of Sciences 109 50 20744 20749 |
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Open Polar |
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DIAL@UCLouvain (Université catholique de Louvain) |
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ftunivlouvain |
language |
English |
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3123 QH301 |
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3123 QH301 Teets, Nicholas M. Peyton, Justin T. Colinet, Hervé Renault, David Kelley, Joanna L. Kawarasaki, Yuta Lee, Richard E. Denlinger, David L. Gene expression changes governing extreme dehydration tolerance in an Antarctic insect |
topic_facet |
3123 QH301 |
description |
Among terrestrial organisms, arthropods are especially susceptible to dehydration, given their small body size and high surface area to volume ratio. This challenge is particularly acute for polar arthropods that face near-constant desiccating conditions, as water is frozen and thus unavailable for much of the year. The molecular mechanisms that govern extreme dehydration tolerance in insects remain largely undefined. In this study, we used RNA sequencing to quantify transcriptional mechanisms of extreme dehydration tolerance in the Antarctic midge, Belgica antarctica, the world's southernmost insect and only insect endemic to Antarctica. Larvae of B. antarctica are remarkably tolerant of dehydration, surviving losses up to 70% of their body water. Gene expression changes in response to dehydration indicated up-regulation of cellular recycling pathways including the ubiquitin-mediated proteasome and autophagy, with concurrent down-regulation of genes involved in general metabolism and ATP production. Metabolomics results revealed shifts in metabolite pools that correlated closely with changes in gene expression, indicating that coordinated changes in gene expression and metabolism are a critical component of the dehydration response. Finally, using comparative genomics, we compared our gene expression results with a transcriptomic dataset for the Arctic collembolan, Megaphorura arctica. Although B. antarctica and M. arctica are adapted to similar environments, our analysis indicated very little overlap in expression profiles between these two arthropods. Whereas several orthologous genes showed similar expression patterns, transcriptional changes were largely species specific, indicating these polar arthropods have developed distinct transcriptional mechanisms to cope with similar desiccating conditions. |
author2 |
UCL - SST/ELI/ELIB - Biodiversity |
format |
Article in Journal/Newspaper |
author |
Teets, Nicholas M. Peyton, Justin T. Colinet, Hervé Renault, David Kelley, Joanna L. Kawarasaki, Yuta Lee, Richard E. Denlinger, David L. |
author_facet |
Teets, Nicholas M. Peyton, Justin T. Colinet, Hervé Renault, David Kelley, Joanna L. Kawarasaki, Yuta Lee, Richard E. Denlinger, David L. |
author_sort |
Teets, Nicholas M. |
title |
Gene expression changes governing extreme dehydration tolerance in an Antarctic insect |
title_short |
Gene expression changes governing extreme dehydration tolerance in an Antarctic insect |
title_full |
Gene expression changes governing extreme dehydration tolerance in an Antarctic insect |
title_fullStr |
Gene expression changes governing extreme dehydration tolerance in an Antarctic insect |
title_full_unstemmed |
Gene expression changes governing extreme dehydration tolerance in an Antarctic insect |
title_sort |
gene expression changes governing extreme dehydration tolerance in an antarctic insect |
publisher |
National academy of sciences |
publishDate |
2012 |
url |
http://hdl.handle.net/2078.1/121667 https://doi.org/10.1073/pnas.1218661109 |
genre |
Antarc* Antarctic Antarctic midge Antarctica Arctic Belgica antarctica |
genre_facet |
Antarc* Antarctic Antarctic midge Antarctica Arctic Belgica antarctica |
op_source |
Proceedings of the National academy of sciences of the United States of America, Vol. 109, no.50, p. 20744-20749 (2012) |
op_relation |
boreal:121667 http://hdl.handle.net/2078.1/121667 doi:10.1073/pnas.1218661109 urn:ISSN:0027-8424 urn:EISSN:1091-6490 |
op_rights |
info:eu-repo/semantics/restrictedAccess |
op_doi |
https://doi.org/10.1073/pnas.1218661109 |
container_title |
Proceedings of the National Academy of Sciences |
container_volume |
109 |
container_issue |
50 |
container_start_page |
20744 |
op_container_end_page |
20749 |
_version_ |
1799468966735773696 |