Cross-species infectivity of H3N8 influenza virus in an experimental infection in swine

• Published in: Journal of Virology
• Main Authors: Solórzano, Alicia, Foni, Emanuela, Córdoba, Lorena, Baratelli, Massimiliano, Razzuoli, Elisabetta, Bilato, Dania, Martín del Burgo, María Ángeles, Perlin, David S., Martínez, Jorge, Martínez-Orellana, Pamela, Fraile Sauce, Lorenzo José, Chiapponi, Chiara, Amadori, Massimo, Del Real, Gustavo, Montoya, Maria
• Format: Article in Journal/Newspaper
• Language: English
• Published: American Society for Microbiology 2018
• Subjects: Porcs > Infeccions; Animals > Malalties; Porcs > Virus; harbor seal
• Online Access: http://hdl.handle.net/10459.1/65208; https://doi.org/10.1128/JVI.01509-15

Avian influenza A viruses have gained increasing attention due to their ability to cross the species barrier and cause severe disease in humans and other mammal species as pigs. H3 and particularly H3N8 viruses, are highly adaptive since they are found in multiple avian and mammal hosts. H3N8 viruses have not been isolated yet from humans; however, a recent report showed that equine influenza A viruses (IAVs) can be isolated from pigs, although an established infection has not been observed thus far in this host. To gain insight into the possibility of H3N8 avian IAVs to cross the species barrier into pigs, in vitro experiments and an experimental infection in pigs with four H3N8 viruses from different origins (equine, canine, avian, and seal) were performed. As a positive control, an H3N2 swine influenza virus A was used. Although equine and canine viruses hardly replicated in the respiratory systems of pigs, avian and seal viruses replicated substantially and caused detectable lesions in inoculated pigs without previous adaptation. Interestingly, antibodies against hemagglutinin could not be detected after infection by hemagglutination inhibition (HAI) test with avian and seal viruses. This phenomenon was observed not only in pigs but also in mice immunized with the same virus strains. Our data indicated that H3N8 IAVs from wild aquatic birds have the potential to cross the species barrier and establish successful infections in pigs that might spread unnoticed using the HAI test as diagnostic tool. We thank Jaime Maldonado and HIPRA (Spain) for the A/Swine/Spain/ 54008/2004 (H3N2) strain, Edward J. Dubovi and Cornell University for the A/Canine/NY/105447/08 (H3N8) IAV strain, T. M. Chambers and the University of Kentucky for the A/Equine/OH/1/03 (H3N8) IAV strain, and Hon Ip and the U.S. Geological Survey National Wildlife Health Center for the A/American black duck/Maine/44411-532/2008 (H3N8) and the A/Harbor Seal/New Hampshire/179629/2011 (H3N8) IAV strains. We thank Sergio López, David Solanes, Francisco ...