Ten-Second Electrophysiology: Evaluation of the 3DEP Platform for high-speed, high-accuracy cell analysis
Electrical correlates of the physiological state of a cell, such as membrane conductance and capacitance, as well as cytoplasm conductivity, contain vital information about cellular function, ion transport across the membrane, and propagation of electrical signals. They are, however, difficult to me...
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ftunivliverpool:oai:livrepository.liverpool.ac.uk:3067080 2023-05-15T15:56:35+02:00 Ten-Second Electrophysiology: Evaluation of the 3DEP Platform for high-speed, high-accuracy cell analysis Hoettges, Kai F Henslee, Erin A Torcal Serrano, Ruth M Jabr, Rita I Abdallat, Rula G Beale, Andrew D Waheed, Abdul Camelliti, Patrizia Fry, Christopher H van der Veen, Daan R Labeed, Fatima H Hughes, Michael P 2019-12-16 text http://livrepository.liverpool.ac.uk/3067080/ https://www.nature.com/articles/s41598-019-55579-9 http://livrepository.liverpool.ac.uk/3067080/1/Hoettges%20et%20al%20Sci%20Rep%202019.docx en eng eng Springer Science and Business Media LLC http://livrepository.liverpool.ac.uk/3067080/1/Hoettges%20et%20al%20Sci%20Rep%202019.docx Collapse authors list. Hoettges, Kai F orcid:0000-0002-0415-1688 , Henslee, Erin A, Torcal Serrano, Ruth M, Jabr, Rita I, Abdallat, Rula G, Beale, Andrew D, Waheed, Abdul, Camelliti, Patrizia, Fry, Christopher H, van der Veen, Daan R et al (show 2 more authors) , Labeed, Fatima H and Hughes, Michael P (2019) Ten-Second Electrophysiology: Evaluation of the 3DEP Platform for high-speed, high-accuracy cell analysis. Scientific Reports, 9 (1). 19153-. Article NonPeerReviewed 2019 ftunivliverpool 2023-01-19T23:50:12Z Electrical correlates of the physiological state of a cell, such as membrane conductance and capacitance, as well as cytoplasm conductivity, contain vital information about cellular function, ion transport across the membrane, and propagation of electrical signals. They are, however, difficult to measure; gold-standard techniques are typically unable to measure more than a few cells per day, making widespread adoption difficult and limiting statistical reproducibility. We have developed a dielectrophoretic platform using a disposable 3D electrode geometry that accurately (r2 > 0.99) measures mean electrical properties of populations of ~20,000 cells, by taking parallel ensemble measurements of cells at 20 frequencies up to 45 MHz, in (typically) ten seconds. This allows acquisition of ultra-high-resolution (100-point) DEP spectra in under two minutes. Data acquired from a wide range of cells – from platelets to large cardiac cells - benchmark well with patch-clamp-data. These advantages are collectively demonstrated in a longitudinal (same-animal) study of rapidly-changing phenomena such as ultradian (2–3 hour) rhythmicity in whole blood samples of the common vole (Microtus arvalis), taken from 10 µl tail-nick blood samples and avoiding sacrifice of the animal that is typically required in these studies. Article in Journal/Newspaper Common vole Microtus arvalis The University of Liverpool Repository |
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The University of Liverpool Repository |
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ftunivliverpool |
language |
English |
description |
Electrical correlates of the physiological state of a cell, such as membrane conductance and capacitance, as well as cytoplasm conductivity, contain vital information about cellular function, ion transport across the membrane, and propagation of electrical signals. They are, however, difficult to measure; gold-standard techniques are typically unable to measure more than a few cells per day, making widespread adoption difficult and limiting statistical reproducibility. We have developed a dielectrophoretic platform using a disposable 3D electrode geometry that accurately (r2 > 0.99) measures mean electrical properties of populations of ~20,000 cells, by taking parallel ensemble measurements of cells at 20 frequencies up to 45 MHz, in (typically) ten seconds. This allows acquisition of ultra-high-resolution (100-point) DEP spectra in under two minutes. Data acquired from a wide range of cells – from platelets to large cardiac cells - benchmark well with patch-clamp-data. These advantages are collectively demonstrated in a longitudinal (same-animal) study of rapidly-changing phenomena such as ultradian (2–3 hour) rhythmicity in whole blood samples of the common vole (Microtus arvalis), taken from 10 µl tail-nick blood samples and avoiding sacrifice of the animal that is typically required in these studies. |
format |
Article in Journal/Newspaper |
author |
Hoettges, Kai F Henslee, Erin A Torcal Serrano, Ruth M Jabr, Rita I Abdallat, Rula G Beale, Andrew D Waheed, Abdul Camelliti, Patrizia Fry, Christopher H van der Veen, Daan R Labeed, Fatima H Hughes, Michael P |
spellingShingle |
Hoettges, Kai F Henslee, Erin A Torcal Serrano, Ruth M Jabr, Rita I Abdallat, Rula G Beale, Andrew D Waheed, Abdul Camelliti, Patrizia Fry, Christopher H van der Veen, Daan R Labeed, Fatima H Hughes, Michael P Ten-Second Electrophysiology: Evaluation of the 3DEP Platform for high-speed, high-accuracy cell analysis |
author_facet |
Hoettges, Kai F Henslee, Erin A Torcal Serrano, Ruth M Jabr, Rita I Abdallat, Rula G Beale, Andrew D Waheed, Abdul Camelliti, Patrizia Fry, Christopher H van der Veen, Daan R Labeed, Fatima H Hughes, Michael P |
author_sort |
Hoettges, Kai F |
title |
Ten-Second Electrophysiology: Evaluation of the 3DEP Platform for high-speed, high-accuracy cell analysis |
title_short |
Ten-Second Electrophysiology: Evaluation of the 3DEP Platform for high-speed, high-accuracy cell analysis |
title_full |
Ten-Second Electrophysiology: Evaluation of the 3DEP Platform for high-speed, high-accuracy cell analysis |
title_fullStr |
Ten-Second Electrophysiology: Evaluation of the 3DEP Platform for high-speed, high-accuracy cell analysis |
title_full_unstemmed |
Ten-Second Electrophysiology: Evaluation of the 3DEP Platform for high-speed, high-accuracy cell analysis |
title_sort |
ten-second electrophysiology: evaluation of the 3dep platform for high-speed, high-accuracy cell analysis |
publisher |
Springer Science and Business Media LLC |
publishDate |
2019 |
url |
http://livrepository.liverpool.ac.uk/3067080/ https://www.nature.com/articles/s41598-019-55579-9 http://livrepository.liverpool.ac.uk/3067080/1/Hoettges%20et%20al%20Sci%20Rep%202019.docx |
genre |
Common vole Microtus arvalis |
genre_facet |
Common vole Microtus arvalis |
op_relation |
http://livrepository.liverpool.ac.uk/3067080/1/Hoettges%20et%20al%20Sci%20Rep%202019.docx Collapse authors list. Hoettges, Kai F orcid:0000-0002-0415-1688 , Henslee, Erin A, Torcal Serrano, Ruth M, Jabr, Rita I, Abdallat, Rula G, Beale, Andrew D, Waheed, Abdul, Camelliti, Patrizia, Fry, Christopher H, van der Veen, Daan R et al (show 2 more authors) , Labeed, Fatima H and Hughes, Michael P (2019) Ten-Second Electrophysiology: Evaluation of the 3DEP Platform for high-speed, high-accuracy cell analysis. Scientific Reports, 9 (1). 19153-. |
_version_ |
1766391983842000896 |