Life on the edge: O2 binding in Atlantic cod red blood cells near their southern distribution limit is not sensitive to temperature or haemoglobin genotype
Atlantic cod are a commercially important species believed to be threatened by warming seas near their southern, equatorward upper thermal edge of distribution. Limitations to circulatory O2 transport, in particular cardiac output, and the geographic distribution of functionally different haemoglobi...
Main Authors: | , , , |
---|---|
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
The Company of Biologists Ltd.
2017
|
Subjects: | |
Online Access: | http://livrepository.liverpool.ac.uk/3041278/ https://jeb.biologists.org/content/220/3/414 http://livrepository.liverpool.ac.uk/3041278/1/Barlow_C1_Manuscript_revised.docx http://livrepository.liverpool.ac.uk/3041278/1/references%20Barlow_C1_Manuscript_revised.docx |
id |
ftunivliverpool:oai:livrepository.liverpool.ac.uk:3041278 |
---|---|
record_format |
openpolar |
spelling |
ftunivliverpool:oai:livrepository.liverpool.ac.uk:3041278 2023-05-15T15:26:50+02:00 Life on the edge: O2 binding in Atlantic cod red blood cells near their southern distribution limit is not sensitive to temperature or haemoglobin genotype Barlow, SL Metcalfe, J Righton, DA Berenbrink, M 2017-02 text http://livrepository.liverpool.ac.uk/3041278/ https://jeb.biologists.org/content/220/3/414 http://livrepository.liverpool.ac.uk/3041278/1/Barlow_C1_Manuscript_revised.docx http://livrepository.liverpool.ac.uk/3041278/1/references%20Barlow_C1_Manuscript_revised.docx en eng eng The Company of Biologists Ltd. http://livrepository.liverpool.ac.uk/3041278/1/Barlow_C1_Manuscript_revised.docx http://livrepository.liverpool.ac.uk/3041278/1/references%20Barlow_C1_Manuscript_revised.docx Barlow, SL, Metcalfe, J, Righton, DA and Berenbrink, M orcid:0000-0002-0793-1313 (2017) Life on the edge: O2 binding in Atlantic cod red blood cells near their southern distribution limit is not sensitive to temperature or haemoglobin genotype. The Journal of Experimental Biology, 220 (3). pp. 414-424. attached Article NonPeerReviewed 2017 ftunivliverpool 2023-01-19T23:40:40Z Atlantic cod are a commercially important species believed to be threatened by warming seas near their southern, equatorward upper thermal edge of distribution. Limitations to circulatory O2 transport, in particular cardiac output, and the geographic distribution of functionally different haemoglobin (Hb) genotypes have separately been suggested to play a role in setting thermal tolerance in this species. The present study assessed the thermal sensitivity of O2 binding in Atlantic cod red blood cells with different Hb genotypes near their upper thermal distribution limit and modelled its consequences for the arterio-venous O2 saturation difference, Sa-vO2 , another major determinant of circulatory O2 supply rate. The results showed statistically indistinguishable red blood cell O2 binding between the three HbI genotypes in wild-caught Atlantic cod from the Irish Sea (53° N). Red blood cells had an unusually low O2 affinity, with reduced or even reversed thermal sensitivity between pH 7.4 and 7.9, and 5.0 and 20.0°C. This was paired with strongly pH-dependent affinity and cooperativity of red blood cell O2 binding (Bohr and Root effects). Modelling of Sa-vO2 at physiological pH, temperature and O2 partial pressures revealed a substantial capacity for increases in Sa-vO2 to meet rising tissue O2 demands at 5.0 and 12.5°C, but not at 20°C. Furthermore, there was no evidence for an increase of maximal Sa-vO2 with temperature. It is suggested that Atlantic cod at such high temperatures may solely depend on increases in cardiac output and blood O2 capacity, or thermal acclimatisation of metabolic rate, for matching circulatory O2 supply to tissue demand. Article in Journal/Newspaper atlantic cod The University of Liverpool Repository |
institution |
Open Polar |
collection |
The University of Liverpool Repository |
op_collection_id |
ftunivliverpool |
language |
English |
description |
Atlantic cod are a commercially important species believed to be threatened by warming seas near their southern, equatorward upper thermal edge of distribution. Limitations to circulatory O2 transport, in particular cardiac output, and the geographic distribution of functionally different haemoglobin (Hb) genotypes have separately been suggested to play a role in setting thermal tolerance in this species. The present study assessed the thermal sensitivity of O2 binding in Atlantic cod red blood cells with different Hb genotypes near their upper thermal distribution limit and modelled its consequences for the arterio-venous O2 saturation difference, Sa-vO2 , another major determinant of circulatory O2 supply rate. The results showed statistically indistinguishable red blood cell O2 binding between the three HbI genotypes in wild-caught Atlantic cod from the Irish Sea (53° N). Red blood cells had an unusually low O2 affinity, with reduced or even reversed thermal sensitivity between pH 7.4 and 7.9, and 5.0 and 20.0°C. This was paired with strongly pH-dependent affinity and cooperativity of red blood cell O2 binding (Bohr and Root effects). Modelling of Sa-vO2 at physiological pH, temperature and O2 partial pressures revealed a substantial capacity for increases in Sa-vO2 to meet rising tissue O2 demands at 5.0 and 12.5°C, but not at 20°C. Furthermore, there was no evidence for an increase of maximal Sa-vO2 with temperature. It is suggested that Atlantic cod at such high temperatures may solely depend on increases in cardiac output and blood O2 capacity, or thermal acclimatisation of metabolic rate, for matching circulatory O2 supply to tissue demand. |
format |
Article in Journal/Newspaper |
author |
Barlow, SL Metcalfe, J Righton, DA Berenbrink, M |
spellingShingle |
Barlow, SL Metcalfe, J Righton, DA Berenbrink, M Life on the edge: O2 binding in Atlantic cod red blood cells near their southern distribution limit is not sensitive to temperature or haemoglobin genotype |
author_facet |
Barlow, SL Metcalfe, J Righton, DA Berenbrink, M |
author_sort |
Barlow, SL |
title |
Life on the edge: O2 binding in Atlantic cod red blood cells near their southern distribution limit is not sensitive to temperature or haemoglobin genotype |
title_short |
Life on the edge: O2 binding in Atlantic cod red blood cells near their southern distribution limit is not sensitive to temperature or haemoglobin genotype |
title_full |
Life on the edge: O2 binding in Atlantic cod red blood cells near their southern distribution limit is not sensitive to temperature or haemoglobin genotype |
title_fullStr |
Life on the edge: O2 binding in Atlantic cod red blood cells near their southern distribution limit is not sensitive to temperature or haemoglobin genotype |
title_full_unstemmed |
Life on the edge: O2 binding in Atlantic cod red blood cells near their southern distribution limit is not sensitive to temperature or haemoglobin genotype |
title_sort |
life on the edge: o2 binding in atlantic cod red blood cells near their southern distribution limit is not sensitive to temperature or haemoglobin genotype |
publisher |
The Company of Biologists Ltd. |
publishDate |
2017 |
url |
http://livrepository.liverpool.ac.uk/3041278/ https://jeb.biologists.org/content/220/3/414 http://livrepository.liverpool.ac.uk/3041278/1/Barlow_C1_Manuscript_revised.docx http://livrepository.liverpool.ac.uk/3041278/1/references%20Barlow_C1_Manuscript_revised.docx |
genre |
atlantic cod |
genre_facet |
atlantic cod |
op_relation |
http://livrepository.liverpool.ac.uk/3041278/1/Barlow_C1_Manuscript_revised.docx http://livrepository.liverpool.ac.uk/3041278/1/references%20Barlow_C1_Manuscript_revised.docx Barlow, SL, Metcalfe, J, Righton, DA and Berenbrink, M orcid:0000-0002-0793-1313 (2017) Life on the edge: O2 binding in Atlantic cod red blood cells near their southern distribution limit is not sensitive to temperature or haemoglobin genotype. The Journal of Experimental Biology, 220 (3). pp. 414-424. |
op_rights |
attached |
_version_ |
1766357319755497472 |