Unveiling patterns of viral pathogen infection in free-ranging carnivores of northern Portugal using a complementary methodological approach
Pathogen surveillance in free-ranging carnivores presents challenges due to their low densitie and secretive nature. We combined molecular and serological assays to investigate infections by viral pathogens (Canine parvovirus (CPV), Canine distemper virus (CDV) and Canine coronavirus (CCoV)) in Port...
Published in: | Comparative Immunology, Microbiology and Infectious Diseases |
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Online Access: | http://hdl.handle.net/10451/45784 https://doi.org/10.1016/j.cimid.2020.101432 |
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ftunivlisboa:oai:repositorio.ul.pt:10451/45784 2024-02-11T10:02:42+01:00 Unveiling patterns of viral pathogen infection in free-ranging carnivores of northern Portugal using a complementary methodological approach Rosa, Gonçalo M. Santos, Nuno Grøndahl-Rosado, Ricardo Petrucci-Fonseca, Francisco Tavares, Luis Neto, Isabel Cartaxeiro, Clara Duarte, Ana 2021-01-16T16:41:59Z http://hdl.handle.net/10451/45784 https://doi.org/10.1016/j.cimid.2020.101432 eng eng Elsevier FCT UID/BIA/00329/2013 (cE3c) FCT UID/CVT/00276/2013 (CIISA) FCT PEst-OE/AGR/UI0276/2014 Rosa G.M., Santos N., Grøndahl-Rosado R., Petrucci-Fonseca, F., Tavares L., Neto I., Cartaxeiro C., Duarte A. 2020. Unveiling patterns of viral pathogen infection in free-ranging carnivores of northern Portugal using a complementary methodological approach. Comparative Immunology, Microbiology and Infectious Diseases DOI:https://doi.org/10.1016/j.cimid.2020.101432 http://hdl.handle.net/10451/45784 doi:10.1016/j.cimid.2020.101432 openAccess Animals Wild Carnivora Female Male Population Surveillance Portugal Virus Diseases Viruses article 2021 ftunivlisboa https://doi.org/10.1016/j.cimid.2020.101432 2024-01-17T01:07:40Z Pathogen surveillance in free-ranging carnivores presents challenges due to their low densitie and secretive nature. We combined molecular and serological assays to investigate infections by viral pathogens (Canine parvovirus (CPV), Canine distemper virus (CDV) and Canine coronavirus (CCoV)) in Portuguese carnivores (Canis lupus, Vulpes vulpes, Lutra lutra, Martes foina, M. martes, Meles meles, and Genetta genetta) over a period of 16 years. Additionally we explored spatio-temporal patterns of virus occurrence in Canis lupus. Our study identified CPV DNA in all carnivore species with an overall prevalence of 91.9 %. CPV was detected in all sampled years and seasons in Canis lupus, supporting its enzootic nature. CDV RNA was mainly detected in the Canidae family, with viral nucleic acid recorded between 2005 and 2008 with a peak prevalence of 67 % among the wolf population, followed by a sharp decline, suggesting an epizootic behaviour of the virus. Antibodies show that mustelids and viverrids were often exposed to CDV. CCoV was first recorded by molecular methods in wolf samples in 2002, remaining in the wolf populations with marked fluctuations over time. The dual serological and molecular approach provided important epidemiological data on pathogens of wild carnivores in Portugal. These programmes should also include monitoring of other potential reservoir hosts such as domestic cats and dogs. info:eu-repo/semantics/publishedVersion Article in Journal/Newspaper Canis lupus Lutra lutra Universidade de Lisboa: repositório.UL Comparative Immunology, Microbiology and Infectious Diseases 69 101432 |
institution |
Open Polar |
collection |
Universidade de Lisboa: repositório.UL |
op_collection_id |
ftunivlisboa |
language |
English |
topic |
Animals Wild Carnivora Female Male Population Surveillance Portugal Virus Diseases Viruses |
spellingShingle |
Animals Wild Carnivora Female Male Population Surveillance Portugal Virus Diseases Viruses Rosa, Gonçalo M. Santos, Nuno Grøndahl-Rosado, Ricardo Petrucci-Fonseca, Francisco Tavares, Luis Neto, Isabel Cartaxeiro, Clara Duarte, Ana Unveiling patterns of viral pathogen infection in free-ranging carnivores of northern Portugal using a complementary methodological approach |
topic_facet |
Animals Wild Carnivora Female Male Population Surveillance Portugal Virus Diseases Viruses |
description |
Pathogen surveillance in free-ranging carnivores presents challenges due to their low densitie and secretive nature. We combined molecular and serological assays to investigate infections by viral pathogens (Canine parvovirus (CPV), Canine distemper virus (CDV) and Canine coronavirus (CCoV)) in Portuguese carnivores (Canis lupus, Vulpes vulpes, Lutra lutra, Martes foina, M. martes, Meles meles, and Genetta genetta) over a period of 16 years. Additionally we explored spatio-temporal patterns of virus occurrence in Canis lupus. Our study identified CPV DNA in all carnivore species with an overall prevalence of 91.9 %. CPV was detected in all sampled years and seasons in Canis lupus, supporting its enzootic nature. CDV RNA was mainly detected in the Canidae family, with viral nucleic acid recorded between 2005 and 2008 with a peak prevalence of 67 % among the wolf population, followed by a sharp decline, suggesting an epizootic behaviour of the virus. Antibodies show that mustelids and viverrids were often exposed to CDV. CCoV was first recorded by molecular methods in wolf samples in 2002, remaining in the wolf populations with marked fluctuations over time. The dual serological and molecular approach provided important epidemiological data on pathogens of wild carnivores in Portugal. These programmes should also include monitoring of other potential reservoir hosts such as domestic cats and dogs. info:eu-repo/semantics/publishedVersion |
format |
Article in Journal/Newspaper |
author |
Rosa, Gonçalo M. Santos, Nuno Grøndahl-Rosado, Ricardo Petrucci-Fonseca, Francisco Tavares, Luis Neto, Isabel Cartaxeiro, Clara Duarte, Ana |
author_facet |
Rosa, Gonçalo M. Santos, Nuno Grøndahl-Rosado, Ricardo Petrucci-Fonseca, Francisco Tavares, Luis Neto, Isabel Cartaxeiro, Clara Duarte, Ana |
author_sort |
Rosa, Gonçalo M. |
title |
Unveiling patterns of viral pathogen infection in free-ranging carnivores of northern Portugal using a complementary methodological approach |
title_short |
Unveiling patterns of viral pathogen infection in free-ranging carnivores of northern Portugal using a complementary methodological approach |
title_full |
Unveiling patterns of viral pathogen infection in free-ranging carnivores of northern Portugal using a complementary methodological approach |
title_fullStr |
Unveiling patterns of viral pathogen infection in free-ranging carnivores of northern Portugal using a complementary methodological approach |
title_full_unstemmed |
Unveiling patterns of viral pathogen infection in free-ranging carnivores of northern Portugal using a complementary methodological approach |
title_sort |
unveiling patterns of viral pathogen infection in free-ranging carnivores of northern portugal using a complementary methodological approach |
publisher |
Elsevier |
publishDate |
2021 |
url |
http://hdl.handle.net/10451/45784 https://doi.org/10.1016/j.cimid.2020.101432 |
genre |
Canis lupus Lutra lutra |
genre_facet |
Canis lupus Lutra lutra |
op_relation |
FCT UID/BIA/00329/2013 (cE3c) FCT UID/CVT/00276/2013 (CIISA) FCT PEst-OE/AGR/UI0276/2014 Rosa G.M., Santos N., Grøndahl-Rosado R., Petrucci-Fonseca, F., Tavares L., Neto I., Cartaxeiro C., Duarte A. 2020. Unveiling patterns of viral pathogen infection in free-ranging carnivores of northern Portugal using a complementary methodological approach. Comparative Immunology, Microbiology and Infectious Diseases DOI:https://doi.org/10.1016/j.cimid.2020.101432 http://hdl.handle.net/10451/45784 doi:10.1016/j.cimid.2020.101432 |
op_rights |
openAccess |
op_doi |
https://doi.org/10.1016/j.cimid.2020.101432 |
container_title |
Comparative Immunology, Microbiology and Infectious Diseases |
container_volume |
69 |
container_start_page |
101432 |
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1790598756890050560 |