Absence of cellular damage in tropical newly hatched sharks (Chiloscyllium plagiosum) under ocean acidification conditions
Sharks have maintained a key role in marine food webs for 400 million years and across varying physicochemical contexts, suggesting plasticity to environmental change. In this study, we investigated the biochemical effects of ocean acidification (OA) levels predicted for 2100 (pCO2 ~ 900 μatm) on ne...
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Online Access: | http://hdl.handle.net/10451/41209 https://doi.org/10.1007/s12192-018-0892-3 |
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ftunivlisboa:oai:repositorio.ul.pt:10451/41209 2023-05-15T17:50:42+02:00 Absence of cellular damage in tropical newly hatched sharks (Chiloscyllium plagiosum) under ocean acidification conditions Lopes, Ana Rita Sampaio, Eduardo Santos, Catarina Couto, Ana Pegado, Maria Rita Diniz, Mário S. Munday, Philip L. Rummer, Jodie L. Rosa, Rui 2020-01-19T20:48:00Z http://hdl.handle.net/10451/41209 https://doi.org/10.1007/s12192-018-0892-3 eng eng Springer Verlag https://link.springer.com/article/10.1007%2Fs12192-018-0892-3 1355-8145 http://hdl.handle.net/10451/41209 doi:10.1007/s12192-018-0892-3 restrictedAccess Carbon dioxide CO2 Elasmobranchs Antioxidant systems Heat shock response Oxidative damage article 2020 ftunivlisboa https://doi.org/10.1007/s12192-018-0892-3 2022-05-25T18:40:01Z Sharks have maintained a key role in marine food webs for 400 million years and across varying physicochemical contexts, suggesting plasticity to environmental change. In this study, we investigated the biochemical effects of ocean acidification (OA) levels predicted for 2100 (pCO2 ~ 900 μatm) on newly hatched tropical whitespotted bamboo sharks (Chiloscyllium plagiosum). Specifically, we measured lipid, protein, and DNA damage levels, as well as changes in the activity of antioxidant enzymes and non-enzymatic ROS scavengers in juvenile sharks exposed to elevated CO2 for 50 days following hatching. Moreover, we also assessed the secondary oxidative stress response, i.e., heat shock response and ubiquitin levels. Newly hatched sharks appear to cope with OA-related stress through a range of tissue-specific biochemical strategies, specifically through the action of antioxidant enzymatic compounds. Our findings suggest that ROS-scavenging molecules, rather than complex enzymatic proteins, provide an effective defense mechanism in dealing with OA-elicited ROS formation. We argue that sharks' ancient antioxidant system, strongly based on non-enzymatic antioxidants (e.g., urea), may provide them with resilience towards OA, potentially beyond the tolerance of more recently evolved species, i.e., teleosts. Nevertheless, previous research has provided evidence of detrimental effects of OA (interacting with other climate-related stressors) on some aspects of shark biology. Moreover, given that long-term acclimation and adaptive potential to rapid environmental changes are yet experimentally unaccounted for, future research is warranted to accurately predict shark physiological performance under future ocean conditions. info:eu-repo/semantics/publishedVersion Article in Journal/Newspaper Ocean acidification Universidade de Lisboa: repositório.UL Cell Stress and Chaperones 23 5 837 846 |
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Open Polar |
collection |
Universidade de Lisboa: repositório.UL |
op_collection_id |
ftunivlisboa |
language |
English |
topic |
Carbon dioxide CO2 Elasmobranchs Antioxidant systems Heat shock response Oxidative damage |
spellingShingle |
Carbon dioxide CO2 Elasmobranchs Antioxidant systems Heat shock response Oxidative damage Lopes, Ana Rita Sampaio, Eduardo Santos, Catarina Couto, Ana Pegado, Maria Rita Diniz, Mário S. Munday, Philip L. Rummer, Jodie L. Rosa, Rui Absence of cellular damage in tropical newly hatched sharks (Chiloscyllium plagiosum) under ocean acidification conditions |
topic_facet |
Carbon dioxide CO2 Elasmobranchs Antioxidant systems Heat shock response Oxidative damage |
description |
Sharks have maintained a key role in marine food webs for 400 million years and across varying physicochemical contexts, suggesting plasticity to environmental change. In this study, we investigated the biochemical effects of ocean acidification (OA) levels predicted for 2100 (pCO2 ~ 900 μatm) on newly hatched tropical whitespotted bamboo sharks (Chiloscyllium plagiosum). Specifically, we measured lipid, protein, and DNA damage levels, as well as changes in the activity of antioxidant enzymes and non-enzymatic ROS scavengers in juvenile sharks exposed to elevated CO2 for 50 days following hatching. Moreover, we also assessed the secondary oxidative stress response, i.e., heat shock response and ubiquitin levels. Newly hatched sharks appear to cope with OA-related stress through a range of tissue-specific biochemical strategies, specifically through the action of antioxidant enzymatic compounds. Our findings suggest that ROS-scavenging molecules, rather than complex enzymatic proteins, provide an effective defense mechanism in dealing with OA-elicited ROS formation. We argue that sharks' ancient antioxidant system, strongly based on non-enzymatic antioxidants (e.g., urea), may provide them with resilience towards OA, potentially beyond the tolerance of more recently evolved species, i.e., teleosts. Nevertheless, previous research has provided evidence of detrimental effects of OA (interacting with other climate-related stressors) on some aspects of shark biology. Moreover, given that long-term acclimation and adaptive potential to rapid environmental changes are yet experimentally unaccounted for, future research is warranted to accurately predict shark physiological performance under future ocean conditions. info:eu-repo/semantics/publishedVersion |
format |
Article in Journal/Newspaper |
author |
Lopes, Ana Rita Sampaio, Eduardo Santos, Catarina Couto, Ana Pegado, Maria Rita Diniz, Mário S. Munday, Philip L. Rummer, Jodie L. Rosa, Rui |
author_facet |
Lopes, Ana Rita Sampaio, Eduardo Santos, Catarina Couto, Ana Pegado, Maria Rita Diniz, Mário S. Munday, Philip L. Rummer, Jodie L. Rosa, Rui |
author_sort |
Lopes, Ana Rita |
title |
Absence of cellular damage in tropical newly hatched sharks (Chiloscyllium plagiosum) under ocean acidification conditions |
title_short |
Absence of cellular damage in tropical newly hatched sharks (Chiloscyllium plagiosum) under ocean acidification conditions |
title_full |
Absence of cellular damage in tropical newly hatched sharks (Chiloscyllium plagiosum) under ocean acidification conditions |
title_fullStr |
Absence of cellular damage in tropical newly hatched sharks (Chiloscyllium plagiosum) under ocean acidification conditions |
title_full_unstemmed |
Absence of cellular damage in tropical newly hatched sharks (Chiloscyllium plagiosum) under ocean acidification conditions |
title_sort |
absence of cellular damage in tropical newly hatched sharks (chiloscyllium plagiosum) under ocean acidification conditions |
publisher |
Springer Verlag |
publishDate |
2020 |
url |
http://hdl.handle.net/10451/41209 https://doi.org/10.1007/s12192-018-0892-3 |
genre |
Ocean acidification |
genre_facet |
Ocean acidification |
op_relation |
https://link.springer.com/article/10.1007%2Fs12192-018-0892-3 1355-8145 http://hdl.handle.net/10451/41209 doi:10.1007/s12192-018-0892-3 |
op_rights |
restrictedAccess |
op_doi |
https://doi.org/10.1007/s12192-018-0892-3 |
container_title |
Cell Stress and Chaperones |
container_volume |
23 |
container_issue |
5 |
container_start_page |
837 |
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846 |
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1766157577699196928 |