Impact of climate change and contamination in the oxidative stress response of marine organismss

• Main Author: Lopes, Ana Rita José
• Other Authors: Rosa, Rui Afonso Bairrão da, 1976-, Diniz, Mário Emanuel Campos de Sousa
• Format: Doctoral or Postdoctoral Thesis
• Language: English
• Published: 2019
• Subjects: Teses de doutoramento - 2018; Domínio/Área Científica::Ciências Naturais::Ciências Biológicas; Canicula; Ocean acidification
• Online Access: http://hdl.handle.net/10451/36936

Tese de doutoramento, Ciências do Mar, Universidade de Lisboa, Faculdade de Ciências, 2018 Atmospheric carbon dioxide (CO2) levels are increasing at an unprecedented rate, changing the carbonate chemistry (in a process known as ocean acidification) and temperature of the worlds ocean. Moreover, the simultaneous occurrence of highly toxic and persistent contaminants, such as mercury, will play a key role in further shaping the ecophysiology of marine organisms. Thus, the main goal of the present dissertation was to undertake the first comprehensive and comparative analysis of the biochemical strategies, namely antioxidant defense (both enzymatic and non-enzymatic antioxidants) and protein repair and removal mechanisms, of several marine organisms – from invertebrate (Veretillum cynomorium and Gammarus locusta) to vertebrate species (Argyrosomus regius, Chiloscyllium plagiosum and Scyliorhinus canicula) – encompassing different life-stages and life-strategies to the predicted climate-mediated changes. The findings provided in the present dissertation proved that organisms’ responses were mostly underpinned by temperature (increasing lipid, protein and nucleic acid damage), that also culminated into increased mercury bioaccumulation and toxicity, while ocean acidification as a sole stressor usually played a minor role in defining species vulnerability (i.e. responsible for increased oxidative damage in the marine calcifying organisms G. locusta). Nonetheless when co-occurring with warming and contamination scenarios, acidification was usually responsible for the reduction of heavy metal accumulation and toxicity, as well as decreased warming and contamination-elicited oxidative stress. Additionally, organisms’ responses were species-specific, and organisms that usually occupy more variable environments (e.g. daily changes in abiotic conditions) usually displayed greater responses towards environmental change than organisms inhabiting more stable environments. Furthermore, and assuming the relevance of ...