Enzymatic production of ecodiesel by using a commercial lipase CALB, immobilized by physical adsorption on mesoporous organosilica materials

• Main Authors: Luna, Carlos, Gascón-Pérez, Victoria, López-Tenllado, Francisco J., Bautista, Felipa M., Verdugo-Escamilla, Cristóbal, Aguado-Deblas, Laura, Calero, Juan, Romero, Antonio A., Luna, Diego, Estévez, Rafael
• Format: Article in Journal/Newspaper
• Language: English
• Published: MDPI 2021
• Subjects: biofuel; ecodiesel; biodiesel; Antarc*; Antarctica
• Online Access: http://hdl.handle.net/10344/10788; https://doi.org/10.3390/catal1111135

peer-reviewed The synthesis of two biocatalysts based on a commercial Candida antarctica lipase B, CALB enzyme (E), physically immobilized on two silica supports, was carried out. The first support was a periodic mesoporous organosilica (PMO) and the second one was a commercial silica modified with octyl groups (octyl-MS3030). The maximum enzyme load was 122 mg enzyme/g support on PMO and 288 mg enzyme/g support on octyl-MS3030. In addition, the biocatalytic efficiency was corroborated by two reaction tests based on the hydrolysis of p-nitrophenylacetate (p-NPA) and tributyrin (TB). The transesterification of sunflower oil with ethanol was carried out over the biocatalysts synthesized at the following reaction conditions: 6 mL sunflower oil, 1.75 mL EtOH, 30 ◦C, 25 µL NaOH 10 N and 300 rpm, attaining conversion values over 80% after 3 h of reaction time. According to the results obtained, we can confirm that these biocatalytic systems are viable candidates to develop, optimize and improve a new methodology to achieve the integration of glycerol in different monoacylglycerol molecules together with fatty acid ethyl esters (FAEE) molecules to obtain Ecodiesel.