Structural elucidation of irish organic farmed salmon (salmo salar) polar lipids with antithrombotic activities
While several marine polar lipids (PL) have exhibited cardioprotective properties through their effects on the platelet-activating factor (PAF) pathways, salmon PL have not been tested so far. In this study, the antithrombotic activities of salmon PL were assessed in human platelets and the structur...
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ftunivlimericfig:oai:figshare.com:article/19820395 2023-05-15T18:09:49+02:00 Structural elucidation of irish organic farmed salmon (salmo salar) polar lipids with antithrombotic activities ALEXANDROS TSOUPRAS Ronan Lordan Martina Demuru Katie Shiels Sushanta Kumar Saha Constantina Nasopoulou IOANNIS ZABETAKIS 2018-01-01T00:00:00Z https://figshare.com/articles/journal_contribution/Structural_elucidation_of_irish_organic_farmed_salmon_salmo_salar_polar_lipids_with_antithrombotic_activities/19820395 unknown 10344/6936 https://figshare.com/articles/journal_contribution/Structural_elucidation_of_irish_organic_farmed_salmon_salmo_salar_polar_lipids_with_antithrombotic_activities/19820395 CC BY-NC-SA 1.0 CC-BY-NC-SA salmon polar lipids platelet aggregation platelet-activating factor (PAF) thrombin antithrombotic LC-MS GC-MS phosphatidylcholine phosphatidylethanolamine polyunsaturated fatty acids (PUFA) docosahexaenoic acid (DHA) Text Journal contribution 2018 ftunivlimericfig 2022-12-28T08:49:26Z While several marine polar lipids (PL) have exhibited cardioprotective properties through their effects on the platelet-activating factor (PAF) pathways, salmon PL have not been tested so far. In this study, the antithrombotic activities of salmon PL were assessed in human platelets and the structural characterisation of bioactive salmon PL was performed by GC-MS and LC-MS analyses. PL from fillets of Irish organic farmed salmon (Salmo salar) were extracted and separated into several lipid subclasses by thin-layer chromatography (TLC), while their fatty acid profile was fully characterised by GC-MS. Salmon total lipids (TL), total neutral lipids (TNL), total polar lipids (TPL), and each PL subclass obtained by TLC were further assessed for their in vitro effects towards PAF-induced and thrombin-induced platelet aggregation in human platelets. Salmon PL exhibited antithrombotic effects on human platelet aggregation, mostly through their strong inhibitory effects against the PAF pathway with IC50 values comparable to other marine PL, but with lower effects towards the thrombin pathway. PL fractions corresponding to phosphatidylcholine and phosphatidylethanolamine derivatives exhibited the most potent anti-PAF effects, while LC-MS analysis putatively elucidated their structure/function relationship. Several diacyl-PC/PE and alkyl-acyl-PC/PE species containing mostly docosahexaenoic acid at their sn-2 glycerol-backbone may be responsible for the bioactivity. The data presented suggests that salmon contains PL with strong antithrombotic bioactivities. Other Non-Article Part of Journal/Newspaper Salmo salar Research from University of Limerick |
institution |
Open Polar |
collection |
Research from University of Limerick |
op_collection_id |
ftunivlimericfig |
language |
unknown |
topic |
salmon polar lipids platelet aggregation platelet-activating factor (PAF) thrombin antithrombotic LC-MS GC-MS phosphatidylcholine phosphatidylethanolamine polyunsaturated fatty acids (PUFA) docosahexaenoic acid (DHA) |
spellingShingle |
salmon polar lipids platelet aggregation platelet-activating factor (PAF) thrombin antithrombotic LC-MS GC-MS phosphatidylcholine phosphatidylethanolamine polyunsaturated fatty acids (PUFA) docosahexaenoic acid (DHA) ALEXANDROS TSOUPRAS Ronan Lordan Martina Demuru Katie Shiels Sushanta Kumar Saha Constantina Nasopoulou IOANNIS ZABETAKIS Structural elucidation of irish organic farmed salmon (salmo salar) polar lipids with antithrombotic activities |
topic_facet |
salmon polar lipids platelet aggregation platelet-activating factor (PAF) thrombin antithrombotic LC-MS GC-MS phosphatidylcholine phosphatidylethanolamine polyunsaturated fatty acids (PUFA) docosahexaenoic acid (DHA) |
description |
While several marine polar lipids (PL) have exhibited cardioprotective properties through their effects on the platelet-activating factor (PAF) pathways, salmon PL have not been tested so far. In this study, the antithrombotic activities of salmon PL were assessed in human platelets and the structural characterisation of bioactive salmon PL was performed by GC-MS and LC-MS analyses. PL from fillets of Irish organic farmed salmon (Salmo salar) were extracted and separated into several lipid subclasses by thin-layer chromatography (TLC), while their fatty acid profile was fully characterised by GC-MS. Salmon total lipids (TL), total neutral lipids (TNL), total polar lipids (TPL), and each PL subclass obtained by TLC were further assessed for their in vitro effects towards PAF-induced and thrombin-induced platelet aggregation in human platelets. Salmon PL exhibited antithrombotic effects on human platelet aggregation, mostly through their strong inhibitory effects against the PAF pathway with IC50 values comparable to other marine PL, but with lower effects towards the thrombin pathway. PL fractions corresponding to phosphatidylcholine and phosphatidylethanolamine derivatives exhibited the most potent anti-PAF effects, while LC-MS analysis putatively elucidated their structure/function relationship. Several diacyl-PC/PE and alkyl-acyl-PC/PE species containing mostly docosahexaenoic acid at their sn-2 glycerol-backbone may be responsible for the bioactivity. The data presented suggests that salmon contains PL with strong antithrombotic bioactivities. |
format |
Other Non-Article Part of Journal/Newspaper |
author |
ALEXANDROS TSOUPRAS Ronan Lordan Martina Demuru Katie Shiels Sushanta Kumar Saha Constantina Nasopoulou IOANNIS ZABETAKIS |
author_facet |
ALEXANDROS TSOUPRAS Ronan Lordan Martina Demuru Katie Shiels Sushanta Kumar Saha Constantina Nasopoulou IOANNIS ZABETAKIS |
author_sort |
ALEXANDROS TSOUPRAS |
title |
Structural elucidation of irish organic farmed salmon (salmo salar) polar lipids with antithrombotic activities |
title_short |
Structural elucidation of irish organic farmed salmon (salmo salar) polar lipids with antithrombotic activities |
title_full |
Structural elucidation of irish organic farmed salmon (salmo salar) polar lipids with antithrombotic activities |
title_fullStr |
Structural elucidation of irish organic farmed salmon (salmo salar) polar lipids with antithrombotic activities |
title_full_unstemmed |
Structural elucidation of irish organic farmed salmon (salmo salar) polar lipids with antithrombotic activities |
title_sort |
structural elucidation of irish organic farmed salmon (salmo salar) polar lipids with antithrombotic activities |
publishDate |
2018 |
url |
https://figshare.com/articles/journal_contribution/Structural_elucidation_of_irish_organic_farmed_salmon_salmo_salar_polar_lipids_with_antithrombotic_activities/19820395 |
genre |
Salmo salar |
genre_facet |
Salmo salar |
op_relation |
10344/6936 https://figshare.com/articles/journal_contribution/Structural_elucidation_of_irish_organic_farmed_salmon_salmo_salar_polar_lipids_with_antithrombotic_activities/19820395 |
op_rights |
CC BY-NC-SA 1.0 |
op_rightsnorm |
CC-BY-NC-SA |
_version_ |
1766182478807040000 |