Species-Specific N-Glycomes and Methylation Patterns of Oysters Crassostrea gigas and Ostrea edulis and Their Possible Consequences for the Norovirus–HBGA Interaction
Noroviruses, the major cause of acute viral gastroenteritis, are known to bind to histo-blood group antigens (HBGAs), including ABH groups and Lewis-type epitopes, which decorate the surface of erythrocytes and epithelial cells of their host tissues. The biosynthesis of these antigens is controlled...
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ftunivlilleoa:oai:lilloa.univ-lille.fr:20.500.12210/86228 2023-11-12T04:16:15+01:00 Species-Specific N-Glycomes and Methylation Patterns of Oysters Crassostrea gigas and Ostrea edulis and Their Possible Consequences for the Norovirus–HBGA Interaction Auger, Audrey Yu, Shin-Yi Guu, Shih-Yun Quéméner, Agnès Euller-Nicolas, Gabriel Ando, Hiromune Desdouits, Marion Le Guyader, Françoise S Khoo, Kay-Hooi Le Pendu, Jacques Chirat, Frederic Guerardel, Yann Université de Lille CNRS Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 UGSF Academia Sinica Centre de Recherche en Cancérologie et Immunologie Intégrée Nantes-Angers CRCI2NA Microbiologie Aliment Santé Environnement MASAE Gifu University National Taïwan University NTU Immunology and New Concepts in ImmunoTherapy INCIT Unité de Glycobiologie Structurale et Fonctionnelle (UGSF) - UMR 8576 2023-09-01T13:20:40Z application/octet-stream application/rdf+xml; charset=utf-8 https://hdl.handle.net/20.500.12210/86228 Anglais eng MDPI 10.3390/md21060342 37367667 Liaison des virus entériques aux glycines et aux huîtres dans l'environnement littoral Marine drugs Mar Drugs http://hdl.handle.net/20.500.12210/86228 Attribution 3.0 United States info:eu-repo/semantics/openAccess Humans;Animals;Norovirus;Crassostrea;Ostrea;Methylation;Ligands;Blood Group Antigens;Epitopes;glycomics;methylation;norovirus ligands;oysters Article original 2023 ftunivlilleoa https://doi.org/20.500.12210/86228 2023-10-25T16:15:24Z Noroviruses, the major cause of acute viral gastroenteritis, are known to bind to histo-blood group antigens (HBGAs), including ABH groups and Lewis-type epitopes, which decorate the surface of erythrocytes and epithelial cells of their host tissues. The biosynthesis of these antigens is controlled by several glycosyltransferases, the distribution and expression of which varies between tissues and individuals. The use of HBGAs as ligands by viruses is not limited to humans, as many animal species, including oysters, which synthesize similar glycan epitopes that act as a gateway for viruses, become vectors for viral infection in humans. Here, we show that different oyster species synthesize a wide range of -glycans that share histo-blood A-antigens but differ in the expression of other terminal antigens and in their modification by -methyl groups. In particular, we show that the -glycans isolated from and exhibit exquisite methylation patterns in their terminal -acetylgalactosamine and fucose residues in terms of position and number, adding another layer of complexity to the post-translational glycosylation modifications of glycoproteins. Furthermore, modeling of the interactions between norovirus capsid proteins and carbohydrate ligands strongly suggests that methylation has the potential to fine-tune the recognition events of oysters by virus particles. 21;6 Article in Journal/Newspaper Crassostrea gigas LillOA (Lille Open Archive - Université de Lille) |
institution |
Open Polar |
collection |
LillOA (Lille Open Archive - Université de Lille) |
op_collection_id |
ftunivlilleoa |
language |
English |
topic |
Humans;Animals;Norovirus;Crassostrea;Ostrea;Methylation;Ligands;Blood Group Antigens;Epitopes;glycomics;methylation;norovirus ligands;oysters |
spellingShingle |
Humans;Animals;Norovirus;Crassostrea;Ostrea;Methylation;Ligands;Blood Group Antigens;Epitopes;glycomics;methylation;norovirus ligands;oysters Auger, Audrey Yu, Shin-Yi Guu, Shih-Yun Quéméner, Agnès Euller-Nicolas, Gabriel Ando, Hiromune Desdouits, Marion Le Guyader, Françoise S Khoo, Kay-Hooi Le Pendu, Jacques Chirat, Frederic Guerardel, Yann Species-Specific N-Glycomes and Methylation Patterns of Oysters Crassostrea gigas and Ostrea edulis and Their Possible Consequences for the Norovirus–HBGA Interaction |
topic_facet |
Humans;Animals;Norovirus;Crassostrea;Ostrea;Methylation;Ligands;Blood Group Antigens;Epitopes;glycomics;methylation;norovirus ligands;oysters |
description |
Noroviruses, the major cause of acute viral gastroenteritis, are known to bind to histo-blood group antigens (HBGAs), including ABH groups and Lewis-type epitopes, which decorate the surface of erythrocytes and epithelial cells of their host tissues. The biosynthesis of these antigens is controlled by several glycosyltransferases, the distribution and expression of which varies between tissues and individuals. The use of HBGAs as ligands by viruses is not limited to humans, as many animal species, including oysters, which synthesize similar glycan epitopes that act as a gateway for viruses, become vectors for viral infection in humans. Here, we show that different oyster species synthesize a wide range of -glycans that share histo-blood A-antigens but differ in the expression of other terminal antigens and in their modification by -methyl groups. In particular, we show that the -glycans isolated from and exhibit exquisite methylation patterns in their terminal -acetylgalactosamine and fucose residues in terms of position and number, adding another layer of complexity to the post-translational glycosylation modifications of glycoproteins. Furthermore, modeling of the interactions between norovirus capsid proteins and carbohydrate ligands strongly suggests that methylation has the potential to fine-tune the recognition events of oysters by virus particles. 21;6 |
author2 |
Université de Lille CNRS Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 UGSF Academia Sinica Centre de Recherche en Cancérologie et Immunologie Intégrée Nantes-Angers CRCI2NA Microbiologie Aliment Santé Environnement MASAE Gifu University National Taïwan University NTU Immunology and New Concepts in ImmunoTherapy INCIT Unité de Glycobiologie Structurale et Fonctionnelle (UGSF) - UMR 8576 |
format |
Article in Journal/Newspaper |
author |
Auger, Audrey Yu, Shin-Yi Guu, Shih-Yun Quéméner, Agnès Euller-Nicolas, Gabriel Ando, Hiromune Desdouits, Marion Le Guyader, Françoise S Khoo, Kay-Hooi Le Pendu, Jacques Chirat, Frederic Guerardel, Yann |
author_facet |
Auger, Audrey Yu, Shin-Yi Guu, Shih-Yun Quéméner, Agnès Euller-Nicolas, Gabriel Ando, Hiromune Desdouits, Marion Le Guyader, Françoise S Khoo, Kay-Hooi Le Pendu, Jacques Chirat, Frederic Guerardel, Yann |
author_sort |
Auger, Audrey |
title |
Species-Specific N-Glycomes and Methylation Patterns of Oysters Crassostrea gigas and Ostrea edulis and Their Possible Consequences for the Norovirus–HBGA Interaction |
title_short |
Species-Specific N-Glycomes and Methylation Patterns of Oysters Crassostrea gigas and Ostrea edulis and Their Possible Consequences for the Norovirus–HBGA Interaction |
title_full |
Species-Specific N-Glycomes and Methylation Patterns of Oysters Crassostrea gigas and Ostrea edulis and Their Possible Consequences for the Norovirus–HBGA Interaction |
title_fullStr |
Species-Specific N-Glycomes and Methylation Patterns of Oysters Crassostrea gigas and Ostrea edulis and Their Possible Consequences for the Norovirus–HBGA Interaction |
title_full_unstemmed |
Species-Specific N-Glycomes and Methylation Patterns of Oysters Crassostrea gigas and Ostrea edulis and Their Possible Consequences for the Norovirus–HBGA Interaction |
title_sort |
species-specific n-glycomes and methylation patterns of oysters crassostrea gigas and ostrea edulis and their possible consequences for the norovirus–hbga interaction |
publisher |
MDPI |
publishDate |
2023 |
url |
https://hdl.handle.net/20.500.12210/86228 |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_relation |
10.3390/md21060342 37367667 Liaison des virus entériques aux glycines et aux huîtres dans l'environnement littoral Marine drugs Mar Drugs http://hdl.handle.net/20.500.12210/86228 |
op_rights |
Attribution 3.0 United States info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/20.500.12210/86228 |
_version_ |
1782333389726023680 |