Evaluation of biological effects of chemical contaminants on juvenile marine fish : a multibiomarqueur approach under laboratory and field conditions
Evaluation of pollutant effects in environment is one of the major issues of the European Water Framework Directive 2000. Regulations have particularly the objective to reach to a good chemical and ecological status of water bodies. In this context, the aim of our study was to develop a multibiomark...
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ftunivlille:oai:HAL:tel-00841588v1 2024-06-23T07:57:20+00:00 Evaluation of biological effects of chemical contaminants on juvenile marine fish : a multibiomarqueur approach under laboratory and field conditions Évaluation des effets biologiques des contaminants chimiques sur les juvéniles de poissons marins : approche multibiomarqueur en conditions expérimentales et in situ Kerambrun, Élodie Laboratoire d’Océanologie et de Géosciences (LOG) - UMR 8187 (LOG) Institut national des sciences de l'Univers (INSU - CNRS)-Université du Littoral Côte d'Opale (ULCO)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD France-Nord ) Université du Littoral Côte d'Opale Rachid Amara Françoise Henry 2011-11-21 https://theses.hal.science/tel-00841588 https://theses.hal.science/tel-00841588/document https://theses.hal.science/tel-00841588/file/KERAMBRUN.pdf fr fre HAL CCSD NNT: 2011DUNK0317 tel-00841588 https://theses.hal.science/tel-00841588 https://theses.hal.science/tel-00841588/document https://theses.hal.science/tel-00841588/file/KERAMBRUN.pdf info:eu-repo/semantics/OpenAccess https://theses.hal.science/tel-00841588 Sciences agricoles. Université du Littoral Côte d'Opale, 2011. Français. ⟨NNT : 2011DUNK0317⟩ Ecotoxicology Juvenile fish Metal PAHs Caging EROD Growth Condition Lipidic index GST Écotoxicologie Juvéniles de poissons Métaux HAPs Biomarqueurs Croissance Indices de condition [SDV.SA]Life Sciences [q-bio]/Agricultural sciences info:eu-repo/semantics/doctoralThesis Theses 2011 ftunivlille 2024-06-10T14:25:16Z Evaluation of pollutant effects in environment is one of the major issues of the European Water Framework Directive 2000. Regulations have particularly the objective to reach to a good chemical and ecological status of water bodies. In this context, the aim of our study was to develop a multibiomarker approach on juvenile marine fish in order to evaluate the biological effects of chemical pollution in coastal areas. Molecular detoxification parameters (EROD, GST) and an antioxidant enzyme (CAT) were used as early warning tools of toxicity allowing the prevention of irreversible damages. In parallel, different physiological biomarkers (somatic and recent growth, RNA:DNA ratio, morphometric and lipidic indices) were analysed as reflecting damages on juveniles health. Sensitivity and relevance of molecular and physiological biomarkers were tested experimentally on juvenile : i) sea bass exposed to acute petroleum pollution, ii) sea bass and turbot submitted to a mix of contaminants in environmental concentrations during controlled and semi-controlled (caging) conditions. Our results show the ability of EROD, and in lower degree the GST, to detect short exposure (2 and 4 days) of organisms to petroleum and to reflect their deleterious effects on fish health. This relationship between molecular and physiological biomarkers was more difficultly established under multiple pollutions. Growth and condition indices were found to be more sensitive to the different levels of chemical contamination analysed (metal, PAHs). Their analyses allowed us to evaluate the weakened condition of organisms caged in the harbour area during 38 days. This caging experiment was relevant especially for juvenile sea bass in which no physiological stress was detected in the reference station. Deleterious effects of chemical contaminant on turbot juvenile health were also observed in controlled condition after 21 days exposure to the same harbour sediments and to an estuarine sediment. In complement to these experiments, a field study was ... Doctoral or Postdoctoral Thesis Turbot LillOA (HAL Lille Open Archive, Université de Lille) |
institution |
Open Polar |
collection |
LillOA (HAL Lille Open Archive, Université de Lille) |
op_collection_id |
ftunivlille |
language |
French |
topic |
Ecotoxicology Juvenile fish Metal PAHs Caging EROD Growth Condition Lipidic index GST Écotoxicologie Juvéniles de poissons Métaux HAPs Biomarqueurs Croissance Indices de condition [SDV.SA]Life Sciences [q-bio]/Agricultural sciences |
spellingShingle |
Ecotoxicology Juvenile fish Metal PAHs Caging EROD Growth Condition Lipidic index GST Écotoxicologie Juvéniles de poissons Métaux HAPs Biomarqueurs Croissance Indices de condition [SDV.SA]Life Sciences [q-bio]/Agricultural sciences Kerambrun, Élodie Evaluation of biological effects of chemical contaminants on juvenile marine fish : a multibiomarqueur approach under laboratory and field conditions |
topic_facet |
Ecotoxicology Juvenile fish Metal PAHs Caging EROD Growth Condition Lipidic index GST Écotoxicologie Juvéniles de poissons Métaux HAPs Biomarqueurs Croissance Indices de condition [SDV.SA]Life Sciences [q-bio]/Agricultural sciences |
description |
Evaluation of pollutant effects in environment is one of the major issues of the European Water Framework Directive 2000. Regulations have particularly the objective to reach to a good chemical and ecological status of water bodies. In this context, the aim of our study was to develop a multibiomarker approach on juvenile marine fish in order to evaluate the biological effects of chemical pollution in coastal areas. Molecular detoxification parameters (EROD, GST) and an antioxidant enzyme (CAT) were used as early warning tools of toxicity allowing the prevention of irreversible damages. In parallel, different physiological biomarkers (somatic and recent growth, RNA:DNA ratio, morphometric and lipidic indices) were analysed as reflecting damages on juveniles health. Sensitivity and relevance of molecular and physiological biomarkers were tested experimentally on juvenile : i) sea bass exposed to acute petroleum pollution, ii) sea bass and turbot submitted to a mix of contaminants in environmental concentrations during controlled and semi-controlled (caging) conditions. Our results show the ability of EROD, and in lower degree the GST, to detect short exposure (2 and 4 days) of organisms to petroleum and to reflect their deleterious effects on fish health. This relationship between molecular and physiological biomarkers was more difficultly established under multiple pollutions. Growth and condition indices were found to be more sensitive to the different levels of chemical contamination analysed (metal, PAHs). Their analyses allowed us to evaluate the weakened condition of organisms caged in the harbour area during 38 days. This caging experiment was relevant especially for juvenile sea bass in which no physiological stress was detected in the reference station. Deleterious effects of chemical contaminant on turbot juvenile health were also observed in controlled condition after 21 days exposure to the same harbour sediments and to an estuarine sediment. In complement to these experiments, a field study was ... |
author2 |
Laboratoire d’Océanologie et de Géosciences (LOG) - UMR 8187 (LOG) Institut national des sciences de l'Univers (INSU - CNRS)-Université du Littoral Côte d'Opale (ULCO)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD France-Nord ) Université du Littoral Côte d'Opale Rachid Amara Françoise Henry |
format |
Doctoral or Postdoctoral Thesis |
author |
Kerambrun, Élodie |
author_facet |
Kerambrun, Élodie |
author_sort |
Kerambrun, Élodie |
title |
Evaluation of biological effects of chemical contaminants on juvenile marine fish : a multibiomarqueur approach under laboratory and field conditions |
title_short |
Evaluation of biological effects of chemical contaminants on juvenile marine fish : a multibiomarqueur approach under laboratory and field conditions |
title_full |
Evaluation of biological effects of chemical contaminants on juvenile marine fish : a multibiomarqueur approach under laboratory and field conditions |
title_fullStr |
Evaluation of biological effects of chemical contaminants on juvenile marine fish : a multibiomarqueur approach under laboratory and field conditions |
title_full_unstemmed |
Evaluation of biological effects of chemical contaminants on juvenile marine fish : a multibiomarqueur approach under laboratory and field conditions |
title_sort |
evaluation of biological effects of chemical contaminants on juvenile marine fish : a multibiomarqueur approach under laboratory and field conditions |
publisher |
HAL CCSD |
publishDate |
2011 |
url |
https://theses.hal.science/tel-00841588 https://theses.hal.science/tel-00841588/document https://theses.hal.science/tel-00841588/file/KERAMBRUN.pdf |
genre |
Turbot |
genre_facet |
Turbot |
op_source |
https://theses.hal.science/tel-00841588 Sciences agricoles. Université du Littoral Côte d'Opale, 2011. Français. ⟨NNT : 2011DUNK0317⟩ |
op_relation |
NNT: 2011DUNK0317 tel-00841588 https://theses.hal.science/tel-00841588 https://theses.hal.science/tel-00841588/document https://theses.hal.science/tel-00841588/file/KERAMBRUN.pdf |
op_rights |
info:eu-repo/semantics/OpenAccess |
_version_ |
1802650922241753088 |