Mucin modified SPR interfaces for studying the effect of flow on pathogen binding to Atlantic salmon mucins
International audience Knowledge on host-pathogen interactions contributes to the development of approaches to alleviate infectious disease. In this work, we developed a surface plasmon resonance (SPR) based method for investigating bacteria/mucins interactions. Furthermore, we investigated adhesion...
Published in: | Biosensors and Bioelectronics |
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Online Access: | https://hal.science/hal-02367926 https://hal.science/hal-02367926/document https://hal.science/hal-02367926/file/Mucin.pdf https://doi.org/10.1016/j.bios.2019.111736 |
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ftunivlille:oai:HAL:hal-02367926v1 2024-02-27T08:38:50+00:00 Mucin modified SPR interfaces for studying the effect of flow on pathogen binding to Atlantic salmon mucins Padra, János, Tamás Pagneux, Quentin Bouckaert, Julie Jijie, Roxana Sundh, Henrik Boukherroub, Rabah Szunerits, Sabine Lindén, Sara Göteborgs Universitet = University of Gothenburg (GU) Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 (IEMN) Centrale Lille-Institut supérieur de l'électronique et du numérique (ISEN)-Université de Valenciennes et du Hainaut-Cambrésis (UVHC)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF) Unité de Glycobiologie Structurale et Fonctionnelle UMR 8576 (UGSF) Université de Lille-Centre National de la Recherche Scientifique (CNRS) NanoBioInterfaces - IEMN (NBI - IEMN) Centrale Lille-Institut supérieur de l'électronique et du numérique (ISEN)-Université de Valenciennes et du Hainaut-Cambrésis (UVHC)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF)-Centrale Lille-Institut supérieur de l'électronique et du numérique (ISEN)-Université de Valenciennes et du Hainaut-Cambrésis (UVHC)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF) European Project: 690836,H2020,H2020-MSCA-RISE-2015,PANG(2016) 2019-12-15 https://hal.science/hal-02367926 https://hal.science/hal-02367926/document https://hal.science/hal-02367926/file/Mucin.pdf https://doi.org/10.1016/j.bios.2019.111736 en eng HAL CCSD Elsevier info:eu-repo/semantics/altIdentifier/doi/10.1016/j.bios.2019.111736 info:eu-repo/grantAgreement//690836/EU/Pathogen and Graphene/PANG hal-02367926 https://hal.science/hal-02367926 https://hal.science/hal-02367926/document https://hal.science/hal-02367926/file/Mucin.pdf doi:10.1016/j.bios.2019.111736 info:eu-repo/semantics/OpenAccess ISSN: 0956-5663 Biosensors and Bioelectronics https://hal.science/hal-02367926 Biosensors and Bioelectronics, 2019, 146, pp.111736. ⟨10.1016/j.bios.2019.111736⟩ surface plasmon resonance mucin Aeromonas salmonicida Vibrio harveyi Aeromonas hydrophila Atlantic salmon [PHYS.PHYS.PHYS-BIO-PH]Physics [physics]/Physics [physics]/Biological Physics [physics.bio-ph] [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology [CHIM.ANAL]Chemical Sciences/Analytical chemistry info:eu-repo/semantics/article Journal articles 2019 ftunivlille https://doi.org/10.1016/j.bios.2019.111736 2024-01-31T18:02:42Z International audience Knowledge on host-pathogen interactions contributes to the development of approaches to alleviate infectious disease. In this work, we developed a surface plasmon resonance (SPR) based method for investigating bacteria/mucins interactions. Furthermore, we investigated adhesion of three pathogens, Aeromonas salmonicida, Aeromonas hydrophila and Vibrio harveyi, to Atlantic salmon mucins isolated from different epithelial sites, using SPR and microtiter-based binding assays. We demonstrated that performing bacterial binding assays to mucins using SPR is feasible and has advantages over microtiter-based binding assays, especially under flow conditions. The fluid flow in the SPR is linear and continuous and SPR enables real-time reading of mucin-bacterial bonds, which provides an in vivo-like setup for analysis of bacterial binding to mucins. The variation between technical replicates was smaller using SPR detection compared to the adenosine 5′-triphosphate (ATP) bioluminescence assay in microtiter plates. Furthermore, we demonstrated that the effect of flow on pathogen-mucin interaction is significant and that bacterial adhesion differ non-linearly with flow rates and depend on the epithelial source of the mucin. Article in Journal/Newspaper Atlantic salmon LillOA (HAL Lille Open Archive, Université de Lille) Biosensors and Bioelectronics 146 111736 |
institution |
Open Polar |
collection |
LillOA (HAL Lille Open Archive, Université de Lille) |
op_collection_id |
ftunivlille |
language |
English |
topic |
surface plasmon resonance mucin Aeromonas salmonicida Vibrio harveyi Aeromonas hydrophila Atlantic salmon [PHYS.PHYS.PHYS-BIO-PH]Physics [physics]/Physics [physics]/Biological Physics [physics.bio-ph] [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology [CHIM.ANAL]Chemical Sciences/Analytical chemistry |
spellingShingle |
surface plasmon resonance mucin Aeromonas salmonicida Vibrio harveyi Aeromonas hydrophila Atlantic salmon [PHYS.PHYS.PHYS-BIO-PH]Physics [physics]/Physics [physics]/Biological Physics [physics.bio-ph] [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology [CHIM.ANAL]Chemical Sciences/Analytical chemistry Padra, János, Tamás Pagneux, Quentin Bouckaert, Julie Jijie, Roxana Sundh, Henrik Boukherroub, Rabah Szunerits, Sabine Lindén, Sara Mucin modified SPR interfaces for studying the effect of flow on pathogen binding to Atlantic salmon mucins |
topic_facet |
surface plasmon resonance mucin Aeromonas salmonicida Vibrio harveyi Aeromonas hydrophila Atlantic salmon [PHYS.PHYS.PHYS-BIO-PH]Physics [physics]/Physics [physics]/Biological Physics [physics.bio-ph] [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology [CHIM.ANAL]Chemical Sciences/Analytical chemistry |
description |
International audience Knowledge on host-pathogen interactions contributes to the development of approaches to alleviate infectious disease. In this work, we developed a surface plasmon resonance (SPR) based method for investigating bacteria/mucins interactions. Furthermore, we investigated adhesion of three pathogens, Aeromonas salmonicida, Aeromonas hydrophila and Vibrio harveyi, to Atlantic salmon mucins isolated from different epithelial sites, using SPR and microtiter-based binding assays. We demonstrated that performing bacterial binding assays to mucins using SPR is feasible and has advantages over microtiter-based binding assays, especially under flow conditions. The fluid flow in the SPR is linear and continuous and SPR enables real-time reading of mucin-bacterial bonds, which provides an in vivo-like setup for analysis of bacterial binding to mucins. The variation between technical replicates was smaller using SPR detection compared to the adenosine 5′-triphosphate (ATP) bioluminescence assay in microtiter plates. Furthermore, we demonstrated that the effect of flow on pathogen-mucin interaction is significant and that bacterial adhesion differ non-linearly with flow rates and depend on the epithelial source of the mucin. |
author2 |
Göteborgs Universitet = University of Gothenburg (GU) Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 (IEMN) Centrale Lille-Institut supérieur de l'électronique et du numérique (ISEN)-Université de Valenciennes et du Hainaut-Cambrésis (UVHC)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF) Unité de Glycobiologie Structurale et Fonctionnelle UMR 8576 (UGSF) Université de Lille-Centre National de la Recherche Scientifique (CNRS) NanoBioInterfaces - IEMN (NBI - IEMN) Centrale Lille-Institut supérieur de l'électronique et du numérique (ISEN)-Université de Valenciennes et du Hainaut-Cambrésis (UVHC)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF)-Centrale Lille-Institut supérieur de l'électronique et du numérique (ISEN)-Université de Valenciennes et du Hainaut-Cambrésis (UVHC)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF) European Project: 690836,H2020,H2020-MSCA-RISE-2015,PANG(2016) |
format |
Article in Journal/Newspaper |
author |
Padra, János, Tamás Pagneux, Quentin Bouckaert, Julie Jijie, Roxana Sundh, Henrik Boukherroub, Rabah Szunerits, Sabine Lindén, Sara |
author_facet |
Padra, János, Tamás Pagneux, Quentin Bouckaert, Julie Jijie, Roxana Sundh, Henrik Boukherroub, Rabah Szunerits, Sabine Lindén, Sara |
author_sort |
Padra, János, Tamás |
title |
Mucin modified SPR interfaces for studying the effect of flow on pathogen binding to Atlantic salmon mucins |
title_short |
Mucin modified SPR interfaces for studying the effect of flow on pathogen binding to Atlantic salmon mucins |
title_full |
Mucin modified SPR interfaces for studying the effect of flow on pathogen binding to Atlantic salmon mucins |
title_fullStr |
Mucin modified SPR interfaces for studying the effect of flow on pathogen binding to Atlantic salmon mucins |
title_full_unstemmed |
Mucin modified SPR interfaces for studying the effect of flow on pathogen binding to Atlantic salmon mucins |
title_sort |
mucin modified spr interfaces for studying the effect of flow on pathogen binding to atlantic salmon mucins |
publisher |
HAL CCSD |
publishDate |
2019 |
url |
https://hal.science/hal-02367926 https://hal.science/hal-02367926/document https://hal.science/hal-02367926/file/Mucin.pdf https://doi.org/10.1016/j.bios.2019.111736 |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_source |
ISSN: 0956-5663 Biosensors and Bioelectronics https://hal.science/hal-02367926 Biosensors and Bioelectronics, 2019, 146, pp.111736. ⟨10.1016/j.bios.2019.111736⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.bios.2019.111736 info:eu-repo/grantAgreement//690836/EU/Pathogen and Graphene/PANG hal-02367926 https://hal.science/hal-02367926 https://hal.science/hal-02367926/document https://hal.science/hal-02367926/file/Mucin.pdf doi:10.1016/j.bios.2019.111736 |
op_rights |
info:eu-repo/semantics/OpenAccess |
op_doi |
https://doi.org/10.1016/j.bios.2019.111736 |
container_title |
Biosensors and Bioelectronics |
container_volume |
146 |
container_start_page |
111736 |
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1792045815898832896 |