Alphacoronaviruses Detected in French Bats Are Phylogeographically Linked to Coronaviruses of European Bats
International audience Bats are a reservoir for a diverse range of viruses, including coronaviruses (CoVs). To determine the presence of CoVs in French bats, fecal samples were collected between July and August of 2014 from four bat species in seven different locations around the city of Bourges in...
Published in: | Viruses |
---|---|
Main Authors: | , , , , , |
Other Authors: | , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
HAL CCSD
2015
|
Subjects: | |
Online Access: | https://hal.science/hal-02266758 https://hal.science/hal-02266758/document https://hal.science/hal-02266758/file/viruses-07-02937.pdf https://doi.org/10.3390/v7122937 |
id |
ftunivlille:oai:HAL:hal-02266758v1 |
---|---|
record_format |
openpolar |
spelling |
ftunivlille:oai:HAL:hal-02266758v1 2024-06-23T07:56:14+00:00 Alphacoronaviruses Detected in French Bats Are Phylogeographically Linked to Coronaviruses of European Bats Goffard, Anne Demanche, Christine Arthur, Laurent Pinçon, Claire Michaux, Johan, J. Dubuisson, Jean Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 (CIIL) Institut Pasteur de Lille Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille-Centre Hospitalier Régional Universitaire CHU Lille (CHRU Lille)-Centre National de la Recherche Scientifique (CNRS) Museum d'Histoire Naturelle de Bourges Evaluation des technologies de santé et des pratiques médicales - ULR 2694 (METRICS) Université de Lille-Centre Hospitalier Régional Universitaire CHU Lille (CHRU Lille) Université de Liège Université de Lille We thank René Courcol, which allowed us to use the facilities of molecular biology platform of the Microbiology Institute of the Biology Pathology Centre of University Hospital of Lille. We are grateful to Anny Dewilde for providing the CoV positive sample as control of pan-coronavirus RT-PCR development. We thank especially Cécile-Marie Aliouat and Annie Standaert who facilitate the contacts between collaborators. 2015 https://hal.science/hal-02266758 https://hal.science/hal-02266758/document https://hal.science/hal-02266758/file/viruses-07-02937.pdf https://doi.org/10.3390/v7122937 en eng HAL CCSD MDPI info:eu-repo/semantics/altIdentifier/doi/10.3390/v7122937 info:eu-repo/semantics/altIdentifier/pmid/26633467 hal-02266758 https://hal.science/hal-02266758 https://hal.science/hal-02266758/document https://hal.science/hal-02266758/file/viruses-07-02937.pdf doi:10.3390/v7122937 PUBMED: 26633467 PUBMEDCENTRAL: PMC4690861 info:eu-repo/semantics/OpenAccess ISSN: 1999-4915 Viruses https://hal.science/hal-02266758 Viruses, 2015, 7 (12), pp.6279-6290. ⟨10.3390/v7122937⟩ Europe molecular characterization phylogenetic analysis phylogeographic analysis coronavirus alphacoronavirus bats [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology [SDV.BID.SPT]Life Sciences [q-bio]/Biodiversity/Systematics Phylogenetics and taxonomy info:eu-repo/semantics/article Journal articles 2015 ftunivlille https://doi.org/10.3390/v7122937 2024-06-10T14:36:34Z International audience Bats are a reservoir for a diverse range of viruses, including coronaviruses (CoVs). To determine the presence of CoVs in French bats, fecal samples were collected between July and August of 2014 from four bat species in seven different locations around the city of Bourges in France. We present for the first time the presence of alpha-CoVs in French Pipistrellus pipistrellus bat species with an estimated prevalence of 4.2%. Based on the analysis of a fragment of the RNA-dependent RNA polymerase (RdRp) gene, phylogenetic analyses show that alpha-CoVs sequences detected in French bats are closely related to other European bat alpha-CoVs. Phylogeographic analyses of RdRp sequences show that several CoVs strains circulate in European bats: (i) old strains detected that have probably diverged a long time ago and are detected in different bat subspecies; (ii) strains detected in Myotis and Pipistrellus bat species that have more recently diverged. Our findings support previous observations describing the complexity of the detected CoVs in bats worldwide Article in Journal/Newspaper Pipistrellus pipistrellus LillOA (HAL Lille Open Archive, Université de Lille) Viruses 7 12 6279 6290 |
institution |
Open Polar |
collection |
LillOA (HAL Lille Open Archive, Université de Lille) |
op_collection_id |
ftunivlille |
language |
English |
topic |
Europe molecular characterization phylogenetic analysis phylogeographic analysis coronavirus alphacoronavirus bats [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology [SDV.BID.SPT]Life Sciences [q-bio]/Biodiversity/Systematics Phylogenetics and taxonomy |
spellingShingle |
Europe molecular characterization phylogenetic analysis phylogeographic analysis coronavirus alphacoronavirus bats [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology [SDV.BID.SPT]Life Sciences [q-bio]/Biodiversity/Systematics Phylogenetics and taxonomy Goffard, Anne Demanche, Christine Arthur, Laurent Pinçon, Claire Michaux, Johan, J. Dubuisson, Jean Alphacoronaviruses Detected in French Bats Are Phylogeographically Linked to Coronaviruses of European Bats |
topic_facet |
Europe molecular characterization phylogenetic analysis phylogeographic analysis coronavirus alphacoronavirus bats [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology [SDV.BID.SPT]Life Sciences [q-bio]/Biodiversity/Systematics Phylogenetics and taxonomy |
description |
International audience Bats are a reservoir for a diverse range of viruses, including coronaviruses (CoVs). To determine the presence of CoVs in French bats, fecal samples were collected between July and August of 2014 from four bat species in seven different locations around the city of Bourges in France. We present for the first time the presence of alpha-CoVs in French Pipistrellus pipistrellus bat species with an estimated prevalence of 4.2%. Based on the analysis of a fragment of the RNA-dependent RNA polymerase (RdRp) gene, phylogenetic analyses show that alpha-CoVs sequences detected in French bats are closely related to other European bat alpha-CoVs. Phylogeographic analyses of RdRp sequences show that several CoVs strains circulate in European bats: (i) old strains detected that have probably diverged a long time ago and are detected in different bat subspecies; (ii) strains detected in Myotis and Pipistrellus bat species that have more recently diverged. Our findings support previous observations describing the complexity of the detected CoVs in bats worldwide |
author2 |
Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 (CIIL) Institut Pasteur de Lille Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille-Centre Hospitalier Régional Universitaire CHU Lille (CHRU Lille)-Centre National de la Recherche Scientifique (CNRS) Museum d'Histoire Naturelle de Bourges Evaluation des technologies de santé et des pratiques médicales - ULR 2694 (METRICS) Université de Lille-Centre Hospitalier Régional Universitaire CHU Lille (CHRU Lille) Université de Liège Université de Lille We thank René Courcol, which allowed us to use the facilities of molecular biology platform of the Microbiology Institute of the Biology Pathology Centre of University Hospital of Lille. We are grateful to Anny Dewilde for providing the CoV positive sample as control of pan-coronavirus RT-PCR development. We thank especially Cécile-Marie Aliouat and Annie Standaert who facilitate the contacts between collaborators. |
format |
Article in Journal/Newspaper |
author |
Goffard, Anne Demanche, Christine Arthur, Laurent Pinçon, Claire Michaux, Johan, J. Dubuisson, Jean |
author_facet |
Goffard, Anne Demanche, Christine Arthur, Laurent Pinçon, Claire Michaux, Johan, J. Dubuisson, Jean |
author_sort |
Goffard, Anne |
title |
Alphacoronaviruses Detected in French Bats Are Phylogeographically Linked to Coronaviruses of European Bats |
title_short |
Alphacoronaviruses Detected in French Bats Are Phylogeographically Linked to Coronaviruses of European Bats |
title_full |
Alphacoronaviruses Detected in French Bats Are Phylogeographically Linked to Coronaviruses of European Bats |
title_fullStr |
Alphacoronaviruses Detected in French Bats Are Phylogeographically Linked to Coronaviruses of European Bats |
title_full_unstemmed |
Alphacoronaviruses Detected in French Bats Are Phylogeographically Linked to Coronaviruses of European Bats |
title_sort |
alphacoronaviruses detected in french bats are phylogeographically linked to coronaviruses of european bats |
publisher |
HAL CCSD |
publishDate |
2015 |
url |
https://hal.science/hal-02266758 https://hal.science/hal-02266758/document https://hal.science/hal-02266758/file/viruses-07-02937.pdf https://doi.org/10.3390/v7122937 |
genre |
Pipistrellus pipistrellus |
genre_facet |
Pipistrellus pipistrellus |
op_source |
ISSN: 1999-4915 Viruses https://hal.science/hal-02266758 Viruses, 2015, 7 (12), pp.6279-6290. ⟨10.3390/v7122937⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.3390/v7122937 info:eu-repo/semantics/altIdentifier/pmid/26633467 hal-02266758 https://hal.science/hal-02266758 https://hal.science/hal-02266758/document https://hal.science/hal-02266758/file/viruses-07-02937.pdf doi:10.3390/v7122937 PUBMED: 26633467 PUBMEDCENTRAL: PMC4690861 |
op_rights |
info:eu-repo/semantics/OpenAccess |
op_doi |
https://doi.org/10.3390/v7122937 |
container_title |
Viruses |
container_volume |
7 |
container_issue |
12 |
container_start_page |
6279 |
op_container_end_page |
6290 |
_version_ |
1802649193449259008 |