Functional characterization of a short neuropeptide F-related receptor in a lophotrochozoan, the mollusk Crassostrea gigas
Members of the short neuropeptide F (sNPF) family of peptides and their cognate receptors play key roles in a variety of physiological processes in arthropods. In silico screening of GigasDatabase, a specific expressed sequence tag database from the Pacific oyster Crassostrea gigas, resulted in the...
Published in: | Journal of Experimental Biology |
---|---|
Main Authors: | , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Published for the Company of Biologists Ltd. by the Cambridge University Press
2014
|
Subjects: | |
Online Access: | https://lirias.kuleuven.be/handle/123456789/445503 https://doi.org/10.1242/jeb.104067 https://lirias.kuleuven.be/bitstream/123456789/445503/2//2974.full.pdf |
id |
ftunivleuven:oai:lirias.kuleuven.be:123456789/445503 |
---|---|
record_format |
openpolar |
spelling |
ftunivleuven:oai:lirias.kuleuven.be:123456789/445503 2023-05-15T15:58:15+02:00 Functional characterization of a short neuropeptide F-related receptor in a lophotrochozoan, the mollusk Crassostrea gigas Bigot, Laetitia Beets, Isabel Dubos, M Boudry, P Schoofs, Liliane Favrel, Pascal 2014-08-15 https://lirias.kuleuven.be/handle/123456789/445503 https://doi.org/10.1242/jeb.104067 https://lirias.kuleuven.be/bitstream/123456789/445503/2//2974.full.pdf en eng Published for the Company of Biologists Ltd. by the Cambridge University Press The Journal of Experimental Biology vol:217 issue:Pt 16 pages:2974-2982 https://lirias.kuleuven.be/handle/123456789/445503 0022-0949 doi:10.1242/jeb.104067 1477-9145 https://lirias.kuleuven.be/bitstream/123456789/445503/2//2974.full.pdf 318421;public Article IT 318421;Article 2014 ftunivleuven https://doi.org/10.1242/jeb.104067 2017-06-02T19:26:26Z Members of the short neuropeptide F (sNPF) family of peptides and their cognate receptors play key roles in a variety of physiological processes in arthropods. In silico screening of GigasDatabase, a specific expressed sequence tag database from the Pacific oyster Crassostrea gigas, resulted in the identification of a receptor (Cg-sNPFR-like) phylogenetically closely related to sNPF receptors (sNPFRs) of insects. A reverse endocrinology approach was undertaken to identify the peptide ligand(s) of this orphan receptor. Though structurally distinct from insect sNPFs, three RFamide peptides derived from the same precursor, i.e. GSLFRFamide, SSLFRFamide and GALFRFamide, specifically activate the receptor in a dose-dependent manner, with respective EC50 values (half-maximal effective concentrations) of 1.1, 2.1 and 4.1 μmol l(-1). We found that both Cg-sNPFR-like receptor and LFRFamide encoding transcripts are expressed in the oyster central nervous system and in other tissues as well, albeit at lower levels. Mass spectrometry analysis confirmed the wide distribution of LFRFamide mature peptides in several central and peripheral tissues. The Cg-sNPFR-like receptor was more abundantly expressed in ganglia of females than of males, and upregulated in starved oysters. In the gonad area, highest receptor gene expression occurred at the start of gametogenesis, when storage activity is maximal. Our results suggest that signaling of LFRFamide peptides through the Cg-sNPFR-like receptor might play a role in the coordination of nutrition, energy storage and metabolism in C. gigas, possibly by promoting storage at the expense of reproduction. status: published Article in Journal/Newspaper Crassostrea gigas Pacific oyster KU Leuven: Lirias Pacific Journal of Experimental Biology |
institution |
Open Polar |
collection |
KU Leuven: Lirias |
op_collection_id |
ftunivleuven |
language |
English |
description |
Members of the short neuropeptide F (sNPF) family of peptides and their cognate receptors play key roles in a variety of physiological processes in arthropods. In silico screening of GigasDatabase, a specific expressed sequence tag database from the Pacific oyster Crassostrea gigas, resulted in the identification of a receptor (Cg-sNPFR-like) phylogenetically closely related to sNPF receptors (sNPFRs) of insects. A reverse endocrinology approach was undertaken to identify the peptide ligand(s) of this orphan receptor. Though structurally distinct from insect sNPFs, three RFamide peptides derived from the same precursor, i.e. GSLFRFamide, SSLFRFamide and GALFRFamide, specifically activate the receptor in a dose-dependent manner, with respective EC50 values (half-maximal effective concentrations) of 1.1, 2.1 and 4.1 μmol l(-1). We found that both Cg-sNPFR-like receptor and LFRFamide encoding transcripts are expressed in the oyster central nervous system and in other tissues as well, albeit at lower levels. Mass spectrometry analysis confirmed the wide distribution of LFRFamide mature peptides in several central and peripheral tissues. The Cg-sNPFR-like receptor was more abundantly expressed in ganglia of females than of males, and upregulated in starved oysters. In the gonad area, highest receptor gene expression occurred at the start of gametogenesis, when storage activity is maximal. Our results suggest that signaling of LFRFamide peptides through the Cg-sNPFR-like receptor might play a role in the coordination of nutrition, energy storage and metabolism in C. gigas, possibly by promoting storage at the expense of reproduction. status: published |
format |
Article in Journal/Newspaper |
author |
Bigot, Laetitia Beets, Isabel Dubos, M Boudry, P Schoofs, Liliane Favrel, Pascal |
spellingShingle |
Bigot, Laetitia Beets, Isabel Dubos, M Boudry, P Schoofs, Liliane Favrel, Pascal Functional characterization of a short neuropeptide F-related receptor in a lophotrochozoan, the mollusk Crassostrea gigas |
author_facet |
Bigot, Laetitia Beets, Isabel Dubos, M Boudry, P Schoofs, Liliane Favrel, Pascal |
author_sort |
Bigot, Laetitia |
title |
Functional characterization of a short neuropeptide F-related receptor in a lophotrochozoan, the mollusk Crassostrea gigas |
title_short |
Functional characterization of a short neuropeptide F-related receptor in a lophotrochozoan, the mollusk Crassostrea gigas |
title_full |
Functional characterization of a short neuropeptide F-related receptor in a lophotrochozoan, the mollusk Crassostrea gigas |
title_fullStr |
Functional characterization of a short neuropeptide F-related receptor in a lophotrochozoan, the mollusk Crassostrea gigas |
title_full_unstemmed |
Functional characterization of a short neuropeptide F-related receptor in a lophotrochozoan, the mollusk Crassostrea gigas |
title_sort |
functional characterization of a short neuropeptide f-related receptor in a lophotrochozoan, the mollusk crassostrea gigas |
publisher |
Published for the Company of Biologists Ltd. by the Cambridge University Press |
publishDate |
2014 |
url |
https://lirias.kuleuven.be/handle/123456789/445503 https://doi.org/10.1242/jeb.104067 https://lirias.kuleuven.be/bitstream/123456789/445503/2//2974.full.pdf |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_relation |
The Journal of Experimental Biology vol:217 issue:Pt 16 pages:2974-2982 https://lirias.kuleuven.be/handle/123456789/445503 0022-0949 doi:10.1242/jeb.104067 1477-9145 https://lirias.kuleuven.be/bitstream/123456789/445503/2//2974.full.pdf |
op_rights |
318421;public |
op_doi |
https://doi.org/10.1242/jeb.104067 |
container_title |
Journal of Experimental Biology |
_version_ |
1766393984781910016 |