Integrating molecular identification of pelagic eggs with geostatistical mapping to improve the delineation of North Sea fish spawning grounds
Maps of the spawning grounds of commercially important fishes are necessary when assessing the level of connectivity between life stages of fishes and for identifying ecologically valuable marine areas. A first step toward mapping the spawning grounds is a reliable and rapid species identification o...
Published in: | Marine Ecology Progress Series |
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Online Access: | https://lirias.kuleuven.be/handle/123456789/353212 https://doi.org/10.3354/meps09443 |
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ftunivleuven:oai:lirias.kuleuven.be:123456789/353212 2023-05-15T16:19:20+02:00 Integrating molecular identification of pelagic eggs with geostatistical mapping to improve the delineation of North Sea fish spawning grounds Lelievre, Stephanie Jerome, Marc Maes, Gregory E Vaz, Sandrine Calaivany, Sachidhanandam Verrez-Bagnis, Veronique 2012 https://lirias.kuleuven.be/handle/123456789/353212 https://doi.org/10.3354/meps09443 en eng Inter-Research. NORDBUNTE 23, D-21385 OLDENDORF LUHE, GERMANY Marine Ecology Progress Series vol:445 pages:161-172 https://lirias.kuleuven.be/handle/123456789/353212 0171-8630 doi:10.3354/meps09443 fish eggs pcr-rflp 16s rrna geostatistical analyses distribution spawning grounds cod gadus-morhua anchovy engraulis-encrasicolus sardine sardina-pilchardus marine reserves species identification community structure taqman probes dna formaldehyde fisheries Description (Metadata) only IT article 2012 ftunivleuven https://doi.org/10.3354/meps09443 2015-12-22T16:20:36Z Maps of the spawning grounds of commercially important fishes are necessary when assessing the level of connectivity between life stages of fishes and for identifying ecologically valuable marine areas. A first step toward mapping the spawning grounds is a reliable and rapid species identification of pelagic fish eggs to assess the spatio-temporal distribution of spawning aggregations. As many species have similar egg sizes and morphology, the molecular validation of visually identified eggs is often essential for the use of such data in fisheries management. In the present study, we developed a rapid 16S rRNA PCR-restriction fragment length polymorphism (RFLP) assay to distinguish between formalin-fixed fish eggs of dab Limanda limanda, flounder Platichthys flesus and pout Trisopterus spp., which were collected during the 2008 International Bottom Trawl Survey in the Eastern English Channel and southern North Sea. A comparison of the rapid 16S rRNA PCR-RFLP method with initial visual identification revealed 93% of correct identifications for dab, 90% for pout, but only 64% for flounder, representing an overall error rate of 17%. Visual misidentification occurred mainly between dab and flounder and between flounder and pout. Egg abundance and the relative proportions of each species were subsequently analysed geostatistically. Molecular identifications were incorporated to obtain corrected interpolated distribution maps, taking into account the results from molecular identifications as a correction factor. This highlighted the distinct spawning grounds for the 3 studied taxa and facilitated the identification of regions of high conservation value for these species. status: published Article in Journal/Newspaper Gadus morhua KU Leuven: Lirias Marine Ecology Progress Series 445 161 172 |
institution |
Open Polar |
collection |
KU Leuven: Lirias |
op_collection_id |
ftunivleuven |
language |
English |
topic |
fish eggs pcr-rflp 16s rrna geostatistical analyses distribution spawning grounds cod gadus-morhua anchovy engraulis-encrasicolus sardine sardina-pilchardus marine reserves species identification community structure taqman probes dna formaldehyde fisheries |
spellingShingle |
fish eggs pcr-rflp 16s rrna geostatistical analyses distribution spawning grounds cod gadus-morhua anchovy engraulis-encrasicolus sardine sardina-pilchardus marine reserves species identification community structure taqman probes dna formaldehyde fisheries Lelievre, Stephanie Jerome, Marc Maes, Gregory E Vaz, Sandrine Calaivany, Sachidhanandam Verrez-Bagnis, Veronique Integrating molecular identification of pelagic eggs with geostatistical mapping to improve the delineation of North Sea fish spawning grounds |
topic_facet |
fish eggs pcr-rflp 16s rrna geostatistical analyses distribution spawning grounds cod gadus-morhua anchovy engraulis-encrasicolus sardine sardina-pilchardus marine reserves species identification community structure taqman probes dna formaldehyde fisheries |
description |
Maps of the spawning grounds of commercially important fishes are necessary when assessing the level of connectivity between life stages of fishes and for identifying ecologically valuable marine areas. A first step toward mapping the spawning grounds is a reliable and rapid species identification of pelagic fish eggs to assess the spatio-temporal distribution of spawning aggregations. As many species have similar egg sizes and morphology, the molecular validation of visually identified eggs is often essential for the use of such data in fisheries management. In the present study, we developed a rapid 16S rRNA PCR-restriction fragment length polymorphism (RFLP) assay to distinguish between formalin-fixed fish eggs of dab Limanda limanda, flounder Platichthys flesus and pout Trisopterus spp., which were collected during the 2008 International Bottom Trawl Survey in the Eastern English Channel and southern North Sea. A comparison of the rapid 16S rRNA PCR-RFLP method with initial visual identification revealed 93% of correct identifications for dab, 90% for pout, but only 64% for flounder, representing an overall error rate of 17%. Visual misidentification occurred mainly between dab and flounder and between flounder and pout. Egg abundance and the relative proportions of each species were subsequently analysed geostatistically. Molecular identifications were incorporated to obtain corrected interpolated distribution maps, taking into account the results from molecular identifications as a correction factor. This highlighted the distinct spawning grounds for the 3 studied taxa and facilitated the identification of regions of high conservation value for these species. status: published |
format |
Article in Journal/Newspaper |
author |
Lelievre, Stephanie Jerome, Marc Maes, Gregory E Vaz, Sandrine Calaivany, Sachidhanandam Verrez-Bagnis, Veronique |
author_facet |
Lelievre, Stephanie Jerome, Marc Maes, Gregory E Vaz, Sandrine Calaivany, Sachidhanandam Verrez-Bagnis, Veronique |
author_sort |
Lelievre, Stephanie |
title |
Integrating molecular identification of pelagic eggs with geostatistical mapping to improve the delineation of North Sea fish spawning grounds |
title_short |
Integrating molecular identification of pelagic eggs with geostatistical mapping to improve the delineation of North Sea fish spawning grounds |
title_full |
Integrating molecular identification of pelagic eggs with geostatistical mapping to improve the delineation of North Sea fish spawning grounds |
title_fullStr |
Integrating molecular identification of pelagic eggs with geostatistical mapping to improve the delineation of North Sea fish spawning grounds |
title_full_unstemmed |
Integrating molecular identification of pelagic eggs with geostatistical mapping to improve the delineation of North Sea fish spawning grounds |
title_sort |
integrating molecular identification of pelagic eggs with geostatistical mapping to improve the delineation of north sea fish spawning grounds |
publisher |
Inter-Research. |
publishDate |
2012 |
url |
https://lirias.kuleuven.be/handle/123456789/353212 https://doi.org/10.3354/meps09443 |
genre |
Gadus morhua |
genre_facet |
Gadus morhua |
op_relation |
Marine Ecology Progress Series vol:445 pages:161-172 https://lirias.kuleuven.be/handle/123456789/353212 0171-8630 doi:10.3354/meps09443 |
op_doi |
https://doi.org/10.3354/meps09443 |
container_title |
Marine Ecology Progress Series |
container_volume |
445 |
container_start_page |
161 |
op_container_end_page |
172 |
_version_ |
1766005718842867712 |