Extracting a century of preserved molecular and population demographic data from archived otoliths in the endangered European eel (Anguilla anguilla L.)
Archived otolith collections represent an invaluable source of information to study demographic and genetic changes in commercially important fish populations. Studies combining both approaches are however rare and reliable extraction of molecular and population demographic data from the same collec...
Published in: | Journal of Experimental Marine Biology and Ecology |
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Language: | English |
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2011
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Online Access: | https://lirias.kuleuven.be/handle/123456789/302225 https://doi.org/10.1016/j.jembe.2010.12.010 https://lirias.kuleuven.be/bitstream/123456789/302225/1//Schaerlaekens+et+al+2011+-+JEMBE.pdf |
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ftunivleuven:oai:lirias.kuleuven.be:123456789/302225 2023-05-15T13:26:50+02:00 Extracting a century of preserved molecular and population demographic data from archived otoliths in the endangered European eel (Anguilla anguilla L.) Schaerlaekens, Dirk Dekker, Willem Wickström, Håkan Volckaert, Filip Maes, Gregory 2011-02-28 501377 bytes application/pdf https://lirias.kuleuven.be/handle/123456789/302225 https://doi.org/10.1016/j.jembe.2010.12.010 https://lirias.kuleuven.be/bitstream/123456789/302225/1//Schaerlaekens+et+al+2011+-+JEMBE.pdf en eng Elsevier Journal of Experimental Marine Biology and Ecology vol:398 issue:1-2 pages:56-62 https://lirias.kuleuven.be/handle/123456789/302225 0022-0981 doi:10.1016/j.jembe.2010.12.010 1879-1697 https://lirias.kuleuven.be/bitstream/123456789/302225/1//Schaerlaekens+et+al+2011+-+JEMBE.pdf 146058;private Otoliths Age determination Archived DNA Microsatellite genotyping European eel Anguilla anguilla Article IT 146058;Article 2011 ftunivleuven https://doi.org/10.1016/j.jembe.2010.12.010 2015-12-22T16:09:03Z Archived otolith collections represent an invaluable source of information to study demographic and genetic changes in commercially important fish populations. Studies combining both approaches are however rare and reliable extraction of molecular and population demographic data from the same collection of otoliths has never been assessed in the endangered European eel (Anguilla anguilla L.). Here we evaluate various DNA extraction protocols to compare DNA yield, microsatellite amplification success, genotype integrity and precision of age determination for eel otoliths that have been archived for 4 weeks, and for 28 and 48 years. Our results show a high amplification success and an equal genotype integrity for DNA fragments extracted from both recently sampled otoliths and high quality reference DNA tissue. Although historical samples yielded low amounts of DNA, PCR amplification was successful and genotyping reliable for short fragments, but decreased significantly with PCR fragment size. None of the extraction protocols caused physical damage to the otoliths and precision of age determination was high for both treated and untreated otoliths. Hence, the methodology can be applied as a standard for the further joint analysis of past demographic and genetic changes during the last century in the highly exploited European eel and in other fish requiring urgent conservation measures. status: published Article in Journal/Newspaper Anguilla anguilla KU Leuven: Lirias Journal of Experimental Marine Biology and Ecology 398 1-2 56 62 |
institution |
Open Polar |
collection |
KU Leuven: Lirias |
op_collection_id |
ftunivleuven |
language |
English |
topic |
Otoliths Age determination Archived DNA Microsatellite genotyping European eel Anguilla anguilla |
spellingShingle |
Otoliths Age determination Archived DNA Microsatellite genotyping European eel Anguilla anguilla Schaerlaekens, Dirk Dekker, Willem Wickström, Håkan Volckaert, Filip Maes, Gregory Extracting a century of preserved molecular and population demographic data from archived otoliths in the endangered European eel (Anguilla anguilla L.) |
topic_facet |
Otoliths Age determination Archived DNA Microsatellite genotyping European eel Anguilla anguilla |
description |
Archived otolith collections represent an invaluable source of information to study demographic and genetic changes in commercially important fish populations. Studies combining both approaches are however rare and reliable extraction of molecular and population demographic data from the same collection of otoliths has never been assessed in the endangered European eel (Anguilla anguilla L.). Here we evaluate various DNA extraction protocols to compare DNA yield, microsatellite amplification success, genotype integrity and precision of age determination for eel otoliths that have been archived for 4 weeks, and for 28 and 48 years. Our results show a high amplification success and an equal genotype integrity for DNA fragments extracted from both recently sampled otoliths and high quality reference DNA tissue. Although historical samples yielded low amounts of DNA, PCR amplification was successful and genotyping reliable for short fragments, but decreased significantly with PCR fragment size. None of the extraction protocols caused physical damage to the otoliths and precision of age determination was high for both treated and untreated otoliths. Hence, the methodology can be applied as a standard for the further joint analysis of past demographic and genetic changes during the last century in the highly exploited European eel and in other fish requiring urgent conservation measures. status: published |
format |
Article in Journal/Newspaper |
author |
Schaerlaekens, Dirk Dekker, Willem Wickström, Håkan Volckaert, Filip Maes, Gregory |
author_facet |
Schaerlaekens, Dirk Dekker, Willem Wickström, Håkan Volckaert, Filip Maes, Gregory |
author_sort |
Schaerlaekens, Dirk |
title |
Extracting a century of preserved molecular and population demographic data from archived otoliths in the endangered European eel (Anguilla anguilla L.) |
title_short |
Extracting a century of preserved molecular and population demographic data from archived otoliths in the endangered European eel (Anguilla anguilla L.) |
title_full |
Extracting a century of preserved molecular and population demographic data from archived otoliths in the endangered European eel (Anguilla anguilla L.) |
title_fullStr |
Extracting a century of preserved molecular and population demographic data from archived otoliths in the endangered European eel (Anguilla anguilla L.) |
title_full_unstemmed |
Extracting a century of preserved molecular and population demographic data from archived otoliths in the endangered European eel (Anguilla anguilla L.) |
title_sort |
extracting a century of preserved molecular and population demographic data from archived otoliths in the endangered european eel (anguilla anguilla l.) |
publisher |
Elsevier |
publishDate |
2011 |
url |
https://lirias.kuleuven.be/handle/123456789/302225 https://doi.org/10.1016/j.jembe.2010.12.010 https://lirias.kuleuven.be/bitstream/123456789/302225/1//Schaerlaekens+et+al+2011+-+JEMBE.pdf |
genre |
Anguilla anguilla |
genre_facet |
Anguilla anguilla |
op_relation |
Journal of Experimental Marine Biology and Ecology vol:398 issue:1-2 pages:56-62 https://lirias.kuleuven.be/handle/123456789/302225 0022-0981 doi:10.1016/j.jembe.2010.12.010 1879-1697 https://lirias.kuleuven.be/bitstream/123456789/302225/1//Schaerlaekens+et+al+2011+-+JEMBE.pdf |
op_rights |
146058;private |
op_doi |
https://doi.org/10.1016/j.jembe.2010.12.010 |
container_title |
Journal of Experimental Marine Biology and Ecology |
container_volume |
398 |
container_issue |
1-2 |
container_start_page |
56 |
op_container_end_page |
62 |
_version_ |
1766394725713051648 |