Isolation and characterization of expressed sequence tag-linked microsatellite loci for the European eel (Anguilla anguilla)

A European eel (Anguilla anguilla) expressed sequence tag database consisting of 795 contigs and 4008 singletons was screened for microsatellites sequences. Primers were designed to amplify 96 repeats, of which 86 gave good quality amplification products. Twenty-eight microsatellites were selected f...

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Main Authors: Pujolar, J. M, Maes, Gregory, Van Houdt, J. K. J, Zane, L
Format: Article in Journal/Newspaper
Language:English
Published: Wiley-Blackwell 2009
Subjects:
anguilla
est
european eel
microsatellite
cross-species amplification
est-library
Anguilla anguilla
European eel
Online Access:https://lirias.kuleuven.be/handle/123456789/299191
id ftunivleuven:oai:lirias.kuleuven.be:123456789/299191
record_format openpolar
spelling ftunivleuven:oai:lirias.kuleuven.be:123456789/299191 2023-05-15T13:26:45+02:00 Isolation and characterization of expressed sequence tag-linked microsatellite loci for the European eel (Anguilla anguilla) Pujolar, J. M Maes, Gregory Van Houdt, J. K. J Zane, L 2009-01 https://lirias.kuleuven.be/handle/123456789/299191 en eng Wiley-Blackwell COMMERCE PLACE, 350 MAIN ST, MALDEN 02148, MA USA Molecular Ecology Resources vol:9 issue:1 pages:233-235 https://lirias.kuleuven.be/handle/123456789/299191 1755-098X 1755-0998 anguilla est european eel microsatellite cross-species amplification est-library Description (Metadata) only IT article 2009 ftunivleuven 2015-12-22T16:08:20Z A European eel (Anguilla anguilla) expressed sequence tag database consisting of 795 contigs and 4008 singletons was screened for microsatellites sequences. Primers were designed to amplify 96 repeats, of which 86 gave good quality amplification products. Twenty-eight microsatellites were selected for further microsatellite genotyping. Only two loci were found to be monomorphic; out of the 26 polymorphic loci, number of alleles per locus ranged from two to 14, while the observed and expected heterozygosities ranged from 0.05 to 0.93, and from 0.05 to 0.95, respectively. All 28 primer sets tested revealed positive amplification in American eel (Anguilla rostrata). status: published Article in Journal/Newspaper Anguilla anguilla European eel KU Leuven: Lirias
institution Open Polar
collection KU Leuven: Lirias
op_collection_id ftunivleuven
language English
topic anguilla
est
european eel
microsatellite
cross-species amplification
est-library
spellingShingle anguilla
est
european eel
microsatellite
cross-species amplification
est-library
Pujolar, J. M
Maes, Gregory
Van Houdt, J. K. J
Zane, L
Isolation and characterization of expressed sequence tag-linked microsatellite loci for the European eel (Anguilla anguilla)
topic_facet anguilla
est
european eel
microsatellite
cross-species amplification
est-library
description A European eel (Anguilla anguilla) expressed sequence tag database consisting of 795 contigs and 4008 singletons was screened for microsatellites sequences. Primers were designed to amplify 96 repeats, of which 86 gave good quality amplification products. Twenty-eight microsatellites were selected for further microsatellite genotyping. Only two loci were found to be monomorphic; out of the 26 polymorphic loci, number of alleles per locus ranged from two to 14, while the observed and expected heterozygosities ranged from 0.05 to 0.93, and from 0.05 to 0.95, respectively. All 28 primer sets tested revealed positive amplification in American eel (Anguilla rostrata). status: published
format Article in Journal/Newspaper
author Pujolar, J. M
Maes, Gregory
Van Houdt, J. K. J
Zane, L
author_facet Pujolar, J. M
Maes, Gregory
Van Houdt, J. K. J
Zane, L
author_sort Pujolar, J. M
title Isolation and characterization of expressed sequence tag-linked microsatellite loci for the European eel (Anguilla anguilla)
title_short Isolation and characterization of expressed sequence tag-linked microsatellite loci for the European eel (Anguilla anguilla)
title_full Isolation and characterization of expressed sequence tag-linked microsatellite loci for the European eel (Anguilla anguilla)
title_fullStr Isolation and characterization of expressed sequence tag-linked microsatellite loci for the European eel (Anguilla anguilla)
title_full_unstemmed Isolation and characterization of expressed sequence tag-linked microsatellite loci for the European eel (Anguilla anguilla)
title_sort isolation and characterization of expressed sequence tag-linked microsatellite loci for the european eel (anguilla anguilla)
publisher Wiley-Blackwell
publishDate 2009
url https://lirias.kuleuven.be/handle/123456789/299191
genre Anguilla anguilla
European eel
genre_facet Anguilla anguilla
European eel
op_relation Molecular Ecology Resources vol:9 issue:1 pages:233-235
https://lirias.kuleuven.be/handle/123456789/299191
1755-098X
1755-0998
_version_ 1766394237654401024

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