Isolation and characterization of expressed sequence tag-linked microsatellite loci for the European eel (Anguilla anguilla)
A European eel (Anguilla anguilla) expressed sequence tag database consisting of 795 contigs and 4008 singletons was screened for microsatellites sequences. Primers were designed to amplify 96 repeats, of which 86 gave good quality amplification products. Twenty-eight microsatellites were selected f...
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ftunivleuven:oai:lirias.kuleuven.be:123456789/299191 2023-05-15T13:26:45+02:00 Isolation and characterization of expressed sequence tag-linked microsatellite loci for the European eel (Anguilla anguilla) Pujolar, J. M Maes, Gregory Van Houdt, J. K. J Zane, L 2009-01 https://lirias.kuleuven.be/handle/123456789/299191 en eng Wiley-Blackwell COMMERCE PLACE, 350 MAIN ST, MALDEN 02148, MA USA Molecular Ecology Resources vol:9 issue:1 pages:233-235 https://lirias.kuleuven.be/handle/123456789/299191 1755-098X 1755-0998 anguilla est european eel microsatellite cross-species amplification est-library Description (Metadata) only IT article 2009 ftunivleuven 2015-12-22T16:08:20Z A European eel (Anguilla anguilla) expressed sequence tag database consisting of 795 contigs and 4008 singletons was screened for microsatellites sequences. Primers were designed to amplify 96 repeats, of which 86 gave good quality amplification products. Twenty-eight microsatellites were selected for further microsatellite genotyping. Only two loci were found to be monomorphic; out of the 26 polymorphic loci, number of alleles per locus ranged from two to 14, while the observed and expected heterozygosities ranged from 0.05 to 0.93, and from 0.05 to 0.95, respectively. All 28 primer sets tested revealed positive amplification in American eel (Anguilla rostrata). status: published Article in Journal/Newspaper Anguilla anguilla European eel KU Leuven: Lirias |
institution |
Open Polar |
collection |
KU Leuven: Lirias |
op_collection_id |
ftunivleuven |
language |
English |
topic |
anguilla est european eel microsatellite cross-species amplification est-library |
spellingShingle |
anguilla est european eel microsatellite cross-species amplification est-library Pujolar, J. M Maes, Gregory Van Houdt, J. K. J Zane, L Isolation and characterization of expressed sequence tag-linked microsatellite loci for the European eel (Anguilla anguilla) |
topic_facet |
anguilla est european eel microsatellite cross-species amplification est-library |
description |
A European eel (Anguilla anguilla) expressed sequence tag database consisting of 795 contigs and 4008 singletons was screened for microsatellites sequences. Primers were designed to amplify 96 repeats, of which 86 gave good quality amplification products. Twenty-eight microsatellites were selected for further microsatellite genotyping. Only two loci were found to be monomorphic; out of the 26 polymorphic loci, number of alleles per locus ranged from two to 14, while the observed and expected heterozygosities ranged from 0.05 to 0.93, and from 0.05 to 0.95, respectively. All 28 primer sets tested revealed positive amplification in American eel (Anguilla rostrata). status: published |
format |
Article in Journal/Newspaper |
author |
Pujolar, J. M Maes, Gregory Van Houdt, J. K. J Zane, L |
author_facet |
Pujolar, J. M Maes, Gregory Van Houdt, J. K. J Zane, L |
author_sort |
Pujolar, J. M |
title |
Isolation and characterization of expressed sequence tag-linked microsatellite loci for the European eel (Anguilla anguilla) |
title_short |
Isolation and characterization of expressed sequence tag-linked microsatellite loci for the European eel (Anguilla anguilla) |
title_full |
Isolation and characterization of expressed sequence tag-linked microsatellite loci for the European eel (Anguilla anguilla) |
title_fullStr |
Isolation and characterization of expressed sequence tag-linked microsatellite loci for the European eel (Anguilla anguilla) |
title_full_unstemmed |
Isolation and characterization of expressed sequence tag-linked microsatellite loci for the European eel (Anguilla anguilla) |
title_sort |
isolation and characterization of expressed sequence tag-linked microsatellite loci for the european eel (anguilla anguilla) |
publisher |
Wiley-Blackwell |
publishDate |
2009 |
url |
https://lirias.kuleuven.be/handle/123456789/299191 |
genre |
Anguilla anguilla European eel |
genre_facet |
Anguilla anguilla European eel |
op_relation |
Molecular Ecology Resources vol:9 issue:1 pages:233-235 https://lirias.kuleuven.be/handle/123456789/299191 1755-098X 1755-0998 |
_version_ |
1766394237654401024 |