Transcriptomic and metatranscriptomic approaches to characterizing genes coding for fiber digestion within the rumen ecosystem
xiv leaves : ill. (some col.) 29 cm The rumen microbiome constitutes a unique genetic resource of plant fiber degrading microbial enzymes that could be used for agricultural and industrial purposes. Anaeromyces mucronatus is a poorly characterized anaerobic lignocellulolytic fungus in the rumen. Thi...
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Lethbridge, Alta. : University of Lethbridge, Dept. of Biological Sciences
2013
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ftunivlethb:oai:opus.uleth.ca:10133/3459 2023-05-15T17:54:12+02:00 Transcriptomic and metatranscriptomic approaches to characterizing genes coding for fiber digestion within the rumen ecosystem Wang, Pan Selinger, L. Brent McAllister, Tim A. 2013 application/pdf https://hdl.handle.net/10133/3459 en_CA eng Lethbridge, Alta. : University of Lethbridge, Dept. of Biological Sciences Arts and Science Department of Biological Sciences Thesis (University of Lethbridge. Faculty of Arts and Science) https://hdl.handle.net/10133/3459 Rumen -- Microbiology -- Research Rumen fermentation -- Research Ruminants -- Digestive organs Anaerobic fungi -- Research Nucleotide sequence RNA -- Analysis Microbial metabolism Dissertations Academic Thesis 2013 ftunivlethb 2021-06-27T07:19:34Z xiv leaves : ill. (some col.) 29 cm The rumen microbiome constitutes a unique genetic resource of plant fiber degrading microbial enzymes that could be used for agricultural and industrial purposes. Anaeromyces mucronatus is a poorly characterized anaerobic lignocellulolytic fungus in the rumen. This thesis aimed at better understanding A. mucronatus YE505 and the particle associated rumen microbiota based on transcriptomic and metatranscriptomic approaches. High quality RNA was isolated from the fiber-associated rumen sample based on an improved RNA extraction method. A transcriptomic study was performed to investigate the expression of the fiber degrading system of A. mucronatus YE505, and the functional diversity of the fiber-associated eukaryotes from the rumen of muskoxen (Ovibos moschatus) was explored by a metatranscriptomic study. Much carbohydrate degradation related protein modules were detected. This study established effective approaches to characterizing the functional contents of rumen eukaryotic microbiome as well as rumen fungi, and identified several candidate genes that merit further investigation. Thesis ovibos moschatus University of Lethbridge Institutional Repository |
institution |
Open Polar |
collection |
University of Lethbridge Institutional Repository |
op_collection_id |
ftunivlethb |
language |
English |
topic |
Rumen -- Microbiology -- Research Rumen fermentation -- Research Ruminants -- Digestive organs Anaerobic fungi -- Research Nucleotide sequence RNA -- Analysis Microbial metabolism Dissertations Academic |
spellingShingle |
Rumen -- Microbiology -- Research Rumen fermentation -- Research Ruminants -- Digestive organs Anaerobic fungi -- Research Nucleotide sequence RNA -- Analysis Microbial metabolism Dissertations Academic Wang, Pan Transcriptomic and metatranscriptomic approaches to characterizing genes coding for fiber digestion within the rumen ecosystem |
topic_facet |
Rumen -- Microbiology -- Research Rumen fermentation -- Research Ruminants -- Digestive organs Anaerobic fungi -- Research Nucleotide sequence RNA -- Analysis Microbial metabolism Dissertations Academic |
description |
xiv leaves : ill. (some col.) 29 cm The rumen microbiome constitutes a unique genetic resource of plant fiber degrading microbial enzymes that could be used for agricultural and industrial purposes. Anaeromyces mucronatus is a poorly characterized anaerobic lignocellulolytic fungus in the rumen. This thesis aimed at better understanding A. mucronatus YE505 and the particle associated rumen microbiota based on transcriptomic and metatranscriptomic approaches. High quality RNA was isolated from the fiber-associated rumen sample based on an improved RNA extraction method. A transcriptomic study was performed to investigate the expression of the fiber degrading system of A. mucronatus YE505, and the functional diversity of the fiber-associated eukaryotes from the rumen of muskoxen (Ovibos moschatus) was explored by a metatranscriptomic study. Much carbohydrate degradation related protein modules were detected. This study established effective approaches to characterizing the functional contents of rumen eukaryotic microbiome as well as rumen fungi, and identified several candidate genes that merit further investigation. |
author2 |
Selinger, L. Brent McAllister, Tim A. |
format |
Thesis |
author |
Wang, Pan |
author_facet |
Wang, Pan |
author_sort |
Wang, Pan |
title |
Transcriptomic and metatranscriptomic approaches to characterizing genes coding for fiber digestion within the rumen ecosystem |
title_short |
Transcriptomic and metatranscriptomic approaches to characterizing genes coding for fiber digestion within the rumen ecosystem |
title_full |
Transcriptomic and metatranscriptomic approaches to characterizing genes coding for fiber digestion within the rumen ecosystem |
title_fullStr |
Transcriptomic and metatranscriptomic approaches to characterizing genes coding for fiber digestion within the rumen ecosystem |
title_full_unstemmed |
Transcriptomic and metatranscriptomic approaches to characterizing genes coding for fiber digestion within the rumen ecosystem |
title_sort |
transcriptomic and metatranscriptomic approaches to characterizing genes coding for fiber digestion within the rumen ecosystem |
publisher |
Lethbridge, Alta. : University of Lethbridge, Dept. of Biological Sciences |
publishDate |
2013 |
url |
https://hdl.handle.net/10133/3459 |
genre |
ovibos moschatus |
genre_facet |
ovibos moschatus |
op_relation |
Thesis (University of Lethbridge. Faculty of Arts and Science) https://hdl.handle.net/10133/3459 |
_version_ |
1766161920058982400 |