Salinity tolerance mechanisms of an Arctic Pelagophyte using comparative transcriptomic and gene expression analysis
Little is known at the transcriptional level about microbial eukaryotic adaptations to short-term salinity change. Arctic microalgae are exposed to low salinity due to sea-ice melt and higher salinity with brine channel formation during freeze-up. Here, we investigate the transcriptional response of...
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Springer Nature
2022
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Online Access: | https://hdl.handle.net/20.500.11794/73521 https://doi.org/10.1038/s42003-022-03461-2 |
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ftunivlavalcorp:oai:corpus.ulaval.ca:20.500.11794/73521 2024-06-23T07:49:40+00:00 Salinity tolerance mechanisms of an Arctic Pelagophyte using comparative transcriptomic and gene expression analysis Lovejoy, Connie Kuo, Alan Freyria, Nastasia J. Chovatia, Mansi Johnson, Jenifer Lipzen, Anna Barry, Kerrie W. Grigoriev, Igor V. Arctique 2022-05-30T18:08:24Z application/pdf https://hdl.handle.net/20.500.11794/73521 https://doi.org/10.1038/s42003-022-03461-2 eng eng Springer Nature 2399-3642 http://hdl.handle.net/20.500.11794/73521 doi:10.1038/s42003-022-03461-2 http://purl.org/coar/access_right/c_abf2 Algues marines -- Métabolisme Microalgues -- Métabolisme Mer -- Salinité Expression génique Transcription génétique article de recherche COAR1_1::Texte::Périodique::Revue::Contribution à un journal::Article::Article de recherche 2022 ftunivlavalcorp https://doi.org/20.500.11794/7352110.1038/s42003-022-03461-2 2024-06-10T23:42:53Z Little is known at the transcriptional level about microbial eukaryotic adaptations to short-term salinity change. Arctic microalgae are exposed to low salinity due to sea-ice melt and higher salinity with brine channel formation during freeze-up. Here, we investigate the transcriptional response of an ice-associated microalgae over salinities from 45 to 8. Our results show a bracketed response of differential gene expression when the cultures were exposed to progressively decreasing salinity. Key genes associated with salinity changes were involved in specific metabolic pathways, transcription factors and regulators, protein kinases, carbohydrate active enzymes, and inorganic ion transporters. The pelagophyte seemed to use a strategy involving overexpression of Na+-H+ antiporters and Na+ -Pi symporters as salinity decreases, but the K+ channel complex at higher salinities. Specific adaptation to cold saline arctic conditions was seen with differential expression of several antifreeze proteins, an ice-binding protein and an acyl-esterase involved in cold adaptation. Other/Unknown Material Arctic Arctique* Sea ice Université Laval: CorpusUL Arctic Communications Biology 5 1 |
institution |
Open Polar |
collection |
Université Laval: CorpusUL |
op_collection_id |
ftunivlavalcorp |
language |
English |
topic |
Algues marines -- Métabolisme Microalgues -- Métabolisme Mer -- Salinité Expression génique Transcription génétique |
spellingShingle |
Algues marines -- Métabolisme Microalgues -- Métabolisme Mer -- Salinité Expression génique Transcription génétique Lovejoy, Connie Kuo, Alan Freyria, Nastasia J. Chovatia, Mansi Johnson, Jenifer Lipzen, Anna Barry, Kerrie W. Grigoriev, Igor V. Salinity tolerance mechanisms of an Arctic Pelagophyte using comparative transcriptomic and gene expression analysis |
topic_facet |
Algues marines -- Métabolisme Microalgues -- Métabolisme Mer -- Salinité Expression génique Transcription génétique |
description |
Little is known at the transcriptional level about microbial eukaryotic adaptations to short-term salinity change. Arctic microalgae are exposed to low salinity due to sea-ice melt and higher salinity with brine channel formation during freeze-up. Here, we investigate the transcriptional response of an ice-associated microalgae over salinities from 45 to 8. Our results show a bracketed response of differential gene expression when the cultures were exposed to progressively decreasing salinity. Key genes associated with salinity changes were involved in specific metabolic pathways, transcription factors and regulators, protein kinases, carbohydrate active enzymes, and inorganic ion transporters. The pelagophyte seemed to use a strategy involving overexpression of Na+-H+ antiporters and Na+ -Pi symporters as salinity decreases, but the K+ channel complex at higher salinities. Specific adaptation to cold saline arctic conditions was seen with differential expression of several antifreeze proteins, an ice-binding protein and an acyl-esterase involved in cold adaptation. |
format |
Other/Unknown Material |
author |
Lovejoy, Connie Kuo, Alan Freyria, Nastasia J. Chovatia, Mansi Johnson, Jenifer Lipzen, Anna Barry, Kerrie W. Grigoriev, Igor V. |
author_facet |
Lovejoy, Connie Kuo, Alan Freyria, Nastasia J. Chovatia, Mansi Johnson, Jenifer Lipzen, Anna Barry, Kerrie W. Grigoriev, Igor V. |
author_sort |
Lovejoy, Connie |
title |
Salinity tolerance mechanisms of an Arctic Pelagophyte using comparative transcriptomic and gene expression analysis |
title_short |
Salinity tolerance mechanisms of an Arctic Pelagophyte using comparative transcriptomic and gene expression analysis |
title_full |
Salinity tolerance mechanisms of an Arctic Pelagophyte using comparative transcriptomic and gene expression analysis |
title_fullStr |
Salinity tolerance mechanisms of an Arctic Pelagophyte using comparative transcriptomic and gene expression analysis |
title_full_unstemmed |
Salinity tolerance mechanisms of an Arctic Pelagophyte using comparative transcriptomic and gene expression analysis |
title_sort |
salinity tolerance mechanisms of an arctic pelagophyte using comparative transcriptomic and gene expression analysis |
publisher |
Springer Nature |
publishDate |
2022 |
url |
https://hdl.handle.net/20.500.11794/73521 https://doi.org/10.1038/s42003-022-03461-2 |
op_coverage |
Arctique |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic Arctique* Sea ice |
genre_facet |
Arctic Arctique* Sea ice |
op_relation |
2399-3642 http://hdl.handle.net/20.500.11794/73521 doi:10.1038/s42003-022-03461-2 |
op_rights |
http://purl.org/coar/access_right/c_abf2 |
op_doi |
https://doi.org/20.500.11794/7352110.1038/s42003-022-03461-2 |
container_title |
Communications Biology |
container_volume |
5 |
container_issue |
1 |
_version_ |
1802640237518651392 |