Size-fractionated microbiome structure in subarctic rivers and a coastal plume across DOC and salinity gradients
Little is known about the microbial diversity of rivers that flow across the changing subarctic landscape. Using amplicon sequencing (rRNA and rRNA genes) combined with HPLC pigment analysis and physicochemical measurements, we investigated the diversity of two size fractions of planktonic Bacteria,...
Published in: | Frontiers in Microbiology |
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Online Access: | https://hdl.handle.net/20.500.11794/72523 https://doi.org/10.3389/fmicb.2021.760282 |
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ftunivlavalcorp:oai:corpus.ulaval.ca:20.500.11794/72523 2024-09-09T19:41:50+00:00 Size-fractionated microbiome structure in subarctic rivers and a coastal plume across DOC and salinity gradients Matveev, Alex Lovejoy, Connie Vincent, Warwick F. Blais, Marie-Amélie Baleine, Grande rivière de la (Québec 2022-02-14T14:19:54Z application/pdf https://hdl.handle.net/20.500.11794/72523 https://doi.org/10.3389/fmicb.2021.760282 eng eng Frontiers Media 1664-302X http://hdl.handle.net/20.500.11794/72523 doi:10.3389/fmicb.2021.760282 PMC8762315 http://purl.org/coar/access_right/c_abf2 Bacteria Microbial eukaryotes Permafrost River microbiomes Climate change Salinity Dissolved organic carbon Northern ecosystems Biodiversité d'eau douce Diversité microbienne Panaches (Dynamique des fluides) Biodiversité littorale Salinité Carbone organique dissous article de recherche COAR1_1::Texte::Périodique::Revue::Contribution à un journal::Article::Article de recherche 2022 ftunivlavalcorp https://doi.org/20.500.11794/7252310.3389/fmicb.2021.760282 2024-06-17T23:42:35Z Little is known about the microbial diversity of rivers that flow across the changing subarctic landscape. Using amplicon sequencing (rRNA and rRNA genes) combined with HPLC pigment analysis and physicochemical measurements, we investigated the diversity of two size fractions of planktonic Bacteria, Archaea and microbial eukaryotes along environmental gradients in the Great Whale River (GWR), Canada. This large subarctic river drains an extensive watershed that includes areas of thawing permafrost, and discharges into southeastern Hudson Bay as an extensive plume that gradually mixes with the coastal marine waters. The microbial communities differed by size-fraction (separated with a 3-μm filter), and clustered into three distinct environmental groups: (1) the GWR sites throughout a 150-km sampling transect; (2) the GWR plume in Hudson Bay; and (3) small rivers that flow through degraded permafrost landscapes. There was a downstream increase in taxonomic richness along the GWR, suggesting that sub-catchment inputs influence microbial community structure in the absence of sharp environmental gradients. Microbial community structure shifted across the salinity gradient within the plume, with changes in taxonomic composition and diversity. Rivers flowing through degraded permafrost had distinct physicochemical and microbiome characteristics, with allochthonous dissolved organic carbon explaining part of the variation in community structure. Finally, our analyses of the core microbiome indicated that while a substantial part of all communities consisted of generalists, most taxa had a more limited environmental range and may therefore be sensitive to ongoing change. Other/Unknown Material Great Whale River Hudson Bay permafrost Subarctic Université Laval: CorpusUL Baleine ENVELOPE(140.012,140.012,-66.649,-66.649) Canada Hudson Hudson Bay Frontiers in Microbiology 12 |
institution |
Open Polar |
collection |
Université Laval: CorpusUL |
op_collection_id |
ftunivlavalcorp |
language |
English |
topic |
Bacteria Microbial eukaryotes Permafrost River microbiomes Climate change Salinity Dissolved organic carbon Northern ecosystems Biodiversité d'eau douce Diversité microbienne Panaches (Dynamique des fluides) Biodiversité littorale Salinité Carbone organique dissous |
spellingShingle |
Bacteria Microbial eukaryotes Permafrost River microbiomes Climate change Salinity Dissolved organic carbon Northern ecosystems Biodiversité d'eau douce Diversité microbienne Panaches (Dynamique des fluides) Biodiversité littorale Salinité Carbone organique dissous Matveev, Alex Lovejoy, Connie Vincent, Warwick F. Blais, Marie-Amélie Size-fractionated microbiome structure in subarctic rivers and a coastal plume across DOC and salinity gradients |
topic_facet |
Bacteria Microbial eukaryotes Permafrost River microbiomes Climate change Salinity Dissolved organic carbon Northern ecosystems Biodiversité d'eau douce Diversité microbienne Panaches (Dynamique des fluides) Biodiversité littorale Salinité Carbone organique dissous |
description |
Little is known about the microbial diversity of rivers that flow across the changing subarctic landscape. Using amplicon sequencing (rRNA and rRNA genes) combined with HPLC pigment analysis and physicochemical measurements, we investigated the diversity of two size fractions of planktonic Bacteria, Archaea and microbial eukaryotes along environmental gradients in the Great Whale River (GWR), Canada. This large subarctic river drains an extensive watershed that includes areas of thawing permafrost, and discharges into southeastern Hudson Bay as an extensive plume that gradually mixes with the coastal marine waters. The microbial communities differed by size-fraction (separated with a 3-μm filter), and clustered into three distinct environmental groups: (1) the GWR sites throughout a 150-km sampling transect; (2) the GWR plume in Hudson Bay; and (3) small rivers that flow through degraded permafrost landscapes. There was a downstream increase in taxonomic richness along the GWR, suggesting that sub-catchment inputs influence microbial community structure in the absence of sharp environmental gradients. Microbial community structure shifted across the salinity gradient within the plume, with changes in taxonomic composition and diversity. Rivers flowing through degraded permafrost had distinct physicochemical and microbiome characteristics, with allochthonous dissolved organic carbon explaining part of the variation in community structure. Finally, our analyses of the core microbiome indicated that while a substantial part of all communities consisted of generalists, most taxa had a more limited environmental range and may therefore be sensitive to ongoing change. |
format |
Other/Unknown Material |
author |
Matveev, Alex Lovejoy, Connie Vincent, Warwick F. Blais, Marie-Amélie |
author_facet |
Matveev, Alex Lovejoy, Connie Vincent, Warwick F. Blais, Marie-Amélie |
author_sort |
Matveev, Alex |
title |
Size-fractionated microbiome structure in subarctic rivers and a coastal plume across DOC and salinity gradients |
title_short |
Size-fractionated microbiome structure in subarctic rivers and a coastal plume across DOC and salinity gradients |
title_full |
Size-fractionated microbiome structure in subarctic rivers and a coastal plume across DOC and salinity gradients |
title_fullStr |
Size-fractionated microbiome structure in subarctic rivers and a coastal plume across DOC and salinity gradients |
title_full_unstemmed |
Size-fractionated microbiome structure in subarctic rivers and a coastal plume across DOC and salinity gradients |
title_sort |
size-fractionated microbiome structure in subarctic rivers and a coastal plume across doc and salinity gradients |
publisher |
Frontiers Media |
publishDate |
2022 |
url |
https://hdl.handle.net/20.500.11794/72523 https://doi.org/10.3389/fmicb.2021.760282 |
op_coverage |
Baleine, Grande rivière de la (Québec |
long_lat |
ENVELOPE(140.012,140.012,-66.649,-66.649) |
geographic |
Baleine Canada Hudson Hudson Bay |
geographic_facet |
Baleine Canada Hudson Hudson Bay |
genre |
Great Whale River Hudson Bay permafrost Subarctic |
genre_facet |
Great Whale River Hudson Bay permafrost Subarctic |
op_relation |
1664-302X http://hdl.handle.net/20.500.11794/72523 doi:10.3389/fmicb.2021.760282 PMC8762315 |
op_rights |
http://purl.org/coar/access_right/c_abf2 |
op_doi |
https://doi.org/20.500.11794/7252310.3389/fmicb.2021.760282 |
container_title |
Frontiers in Microbiology |
container_volume |
12 |
_version_ |
1809911146189160448 |