Comparison of gross visual and microscopic assessment of four anatomic sites to monitor Besnoitia tarandi in barren-ground caribou (Rangifer tarandus groenlandicus)
The objective of this study was to establish a standardized protocol to monitor Besnoitia tarandi prevalence and intensity in barren-ground caribou (Rangifer tarandus) herds by: 1) calculating the relative sensitivity and specificity of the gross visual assessment of four anatomical sites compared w...
Published in: | Journal of Wildlife Diseases |
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Language: | English |
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Wildlife Disease Association
2020
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Online Access: | https://hdl.handle.net/20.500.11794/40605 https://doi.org/10.7589/0090-3558-48.3.732 |
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ftunivlavalcorp:oai:corpus.ulaval.ca:20.500.11794/40605 2024-06-23T07:50:45+00:00 Comparison of gross visual and microscopic assessment of four anatomic sites to monitor Besnoitia tarandi in barren-ground caribou (Rangifer tarandus groenlandicus) Brodeur, Vincent Beauchamp, Guy Côté, Steeve D. Kutz, Susan Taillon, Joëlle Simard, Manon Elkin, Brett Ducrocq, Julie Croft, Bruno Campbell, Mitch Cooley, Dorothy Cuyler, Christine Lair, Stéphane 2020-09-11T16:08:28Z application/pdf https://hdl.handle.net/20.500.11794/40605 https://doi.org/10.7589/0090-3558-48.3.732 eng eng Wildlife Disease Association 0090-3558 http://hdl.handle.net/20.500.11794/40605 doi:10.7589/0090-3558-48.3.732 22740539 http://purl.org/coar/access_right/c_16ec Arctic Besnoitia tarandi Caribou Disease Parasite Protozoa Rangifer tarandus Besnoitia Caribou -- Parasites article de recherche COAR1_1::Texte::Périodique::Revue::Contribution à un journal::Article::Article de recherche 2020 ftunivlavalcorp https://doi.org/20.500.11794/4060510.7589/0090-3558-48.3.732 2024-06-03T23:43:59Z The objective of this study was to establish a standardized protocol to monitor Besnoitia tarandi prevalence and intensity in barren-ground caribou (Rangifer tarandus) herds by: 1) calculating the relative sensitivity and specificity of the gross visual assessment of four anatomical sites compared with microscopic evaluation, and 2) determining which of four anatomical sampling sites was the most sensitive for detecting B. tarandi cysts by microscopy. Sampled tissues consisted of the conjunctiva of the left eye and skin sections from the rostrum, metatarsus, and thigh from 312 harvested caribou. Diagnosis of infection with B. tarandi was based on observation of at least one cyst by microscopic examination. For each tissue, the maximal density of cysts (number of B. tarandi cysts/mm(2) in the section examined) was calculated for a measured area consisting of the dermis extending from the epidermis of the skin to the base of the hair follicles and adnexal structures. For the conjunctiva, the entire submucosa was evaluated. Gross visual evaluation markedly underestimated B. tarandi prevalence in caribou with a relative sensitivity ranging from 0.29 in the conjunctiva to 0.13 in the skin section from the thigh, whereas relative specificities ranged from 0.98 to 1.00. The metatarsus and rostrum skin sections had the highest probabilities of cyst detection of all four anatomical sampling sites. The metatarsus harbored significantly higher densities of B. tarandi cysts than the rostrum, thigh, or conjunctiva. In conclusion, microscopic evaluation of a skin section from the anterior aspect of the mid-third portion of the metatarsal region could be used as a standardized comparative indicator of density of B. tarandi infection in Rangifer. Other/Unknown Material Arctic Rangifer tarandus Université Laval: CorpusUL Arctic Journal of Wildlife Diseases 48 3 732 738 |
institution |
Open Polar |
collection |
Université Laval: CorpusUL |
op_collection_id |
ftunivlavalcorp |
language |
English |
topic |
Arctic Besnoitia tarandi Caribou Disease Parasite Protozoa Rangifer tarandus Besnoitia Caribou -- Parasites |
spellingShingle |
Arctic Besnoitia tarandi Caribou Disease Parasite Protozoa Rangifer tarandus Besnoitia Caribou -- Parasites Brodeur, Vincent Beauchamp, Guy Côté, Steeve D. Kutz, Susan Taillon, Joëlle Simard, Manon Elkin, Brett Ducrocq, Julie Croft, Bruno Campbell, Mitch Cooley, Dorothy Cuyler, Christine Lair, Stéphane Comparison of gross visual and microscopic assessment of four anatomic sites to monitor Besnoitia tarandi in barren-ground caribou (Rangifer tarandus groenlandicus) |
topic_facet |
Arctic Besnoitia tarandi Caribou Disease Parasite Protozoa Rangifer tarandus Besnoitia Caribou -- Parasites |
description |
The objective of this study was to establish a standardized protocol to monitor Besnoitia tarandi prevalence and intensity in barren-ground caribou (Rangifer tarandus) herds by: 1) calculating the relative sensitivity and specificity of the gross visual assessment of four anatomical sites compared with microscopic evaluation, and 2) determining which of four anatomical sampling sites was the most sensitive for detecting B. tarandi cysts by microscopy. Sampled tissues consisted of the conjunctiva of the left eye and skin sections from the rostrum, metatarsus, and thigh from 312 harvested caribou. Diagnosis of infection with B. tarandi was based on observation of at least one cyst by microscopic examination. For each tissue, the maximal density of cysts (number of B. tarandi cysts/mm(2) in the section examined) was calculated for a measured area consisting of the dermis extending from the epidermis of the skin to the base of the hair follicles and adnexal structures. For the conjunctiva, the entire submucosa was evaluated. Gross visual evaluation markedly underestimated B. tarandi prevalence in caribou with a relative sensitivity ranging from 0.29 in the conjunctiva to 0.13 in the skin section from the thigh, whereas relative specificities ranged from 0.98 to 1.00. The metatarsus and rostrum skin sections had the highest probabilities of cyst detection of all four anatomical sampling sites. The metatarsus harbored significantly higher densities of B. tarandi cysts than the rostrum, thigh, or conjunctiva. In conclusion, microscopic evaluation of a skin section from the anterior aspect of the mid-third portion of the metatarsal region could be used as a standardized comparative indicator of density of B. tarandi infection in Rangifer. |
format |
Other/Unknown Material |
author |
Brodeur, Vincent Beauchamp, Guy Côté, Steeve D. Kutz, Susan Taillon, Joëlle Simard, Manon Elkin, Brett Ducrocq, Julie Croft, Bruno Campbell, Mitch Cooley, Dorothy Cuyler, Christine Lair, Stéphane |
author_facet |
Brodeur, Vincent Beauchamp, Guy Côté, Steeve D. Kutz, Susan Taillon, Joëlle Simard, Manon Elkin, Brett Ducrocq, Julie Croft, Bruno Campbell, Mitch Cooley, Dorothy Cuyler, Christine Lair, Stéphane |
author_sort |
Brodeur, Vincent |
title |
Comparison of gross visual and microscopic assessment of four anatomic sites to monitor Besnoitia tarandi in barren-ground caribou (Rangifer tarandus groenlandicus) |
title_short |
Comparison of gross visual and microscopic assessment of four anatomic sites to monitor Besnoitia tarandi in barren-ground caribou (Rangifer tarandus groenlandicus) |
title_full |
Comparison of gross visual and microscopic assessment of four anatomic sites to monitor Besnoitia tarandi in barren-ground caribou (Rangifer tarandus groenlandicus) |
title_fullStr |
Comparison of gross visual and microscopic assessment of four anatomic sites to monitor Besnoitia tarandi in barren-ground caribou (Rangifer tarandus groenlandicus) |
title_full_unstemmed |
Comparison of gross visual and microscopic assessment of four anatomic sites to monitor Besnoitia tarandi in barren-ground caribou (Rangifer tarandus groenlandicus) |
title_sort |
comparison of gross visual and microscopic assessment of four anatomic sites to monitor besnoitia tarandi in barren-ground caribou (rangifer tarandus groenlandicus) |
publisher |
Wildlife Disease Association |
publishDate |
2020 |
url |
https://hdl.handle.net/20.500.11794/40605 https://doi.org/10.7589/0090-3558-48.3.732 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic Rangifer tarandus |
genre_facet |
Arctic Rangifer tarandus |
op_relation |
0090-3558 http://hdl.handle.net/20.500.11794/40605 doi:10.7589/0090-3558-48.3.732 22740539 |
op_rights |
http://purl.org/coar/access_right/c_16ec |
op_doi |
https://doi.org/20.500.11794/4060510.7589/0090-3558-48.3.732 |
container_title |
Journal of Wildlife Diseases |
container_volume |
48 |
container_issue |
3 |
container_start_page |
732 |
op_container_end_page |
738 |
_version_ |
1802641659198963712 |