Extreme viral partitioning in a marine-derived high arctic lake
High-latitude, perennially stratified (meromictic) lakes are likely to be especially vulnerable to climate warming because of the importance of ice in maintaining their water column structure and associated distribution of microbial communities. This study aimed to characterize viral abundance, dive...
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American Society for Microbiology
2020
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ftunivlavalcorp:oai:corpus.ulaval.ca:20.500.11794/39428 2024-06-23T07:46:05+00:00 Extreme viral partitioning in a marine-derived high arctic lake Culley, Alexander Girard, Catherine Vincent, Warwick F. Labbé, Myriam C. Arctique Régions polaires 2020-06-01T15:55:14Z application/pdf https://hdl.handle.net/20.500.11794/39428 https://doi.org/10.1128/mSphere.00334-20 eng eng American Society for Microbiology 2379-5042 http://hdl.handle.net/20.500.11794/39428 doi:10.1128/mSphere.00334-20 PMC7227771 http://purl.org/coar/access_right/c_abf2 Aquatic viral ecology Limnology Polar science Viromics Virologie aquatique Limnologie Virus -- Écologie Glace article de recherche COAR1_1::Texte::Périodique::Revue::Contribution à un journal::Article::Article de recherche 2020 ftunivlavalcorp https://doi.org/20.500.11794/3942810.1128/mSphere.00334-20 2024-06-10T23:42:53Z High-latitude, perennially stratified (meromictic) lakes are likely to be especially vulnerable to climate warming because of the importance of ice in maintaining their water column structure and associated distribution of microbial communities. This study aimed to characterize viral abundance, diversity, and distribution in a meromictic lake of marine origin on the far northern coast of Ellesmere Island, in the Canadian High Arctic. We collected triplicate samples for double-stranded DNA (dsDNA) viromics from five depths that encompassed the major features of the lake, as determined by limnological profiling of the water column. Viral abundance and virus-to-prokaryote ratios were highest at greater depths, while bacterial and cyanobacterial counts were greatest in the surface waters. The viral communities from each zone of the lake defined by salinity, temperature, and dissolved oxygen concentrations were markedly distinct, suggesting that there was little exchange of viral types among lake strata. Ten viral assembled genomes were obtained from our libraries, and these also segregated with depth. This well-defined structure of viral communities was consistent with that of potential hosts. Viruses from the monimolimnion, a deep layer of ancient Arctic Ocean seawater, were more diverse and relatively abundant, with few similarities to available viral sequences. The Lake A viral communities also differed from published records from the Arctic Ocean and meromictic Ace Lake in Antarctica. This first characterization of viral diversity from this sentinel environment underscores the microbial richness and complexity of an ecosystem type that is increasingly exposed to major perturbations in the fast-changing Arctic. Other/Unknown Material Antarc* Antarctica Arctic Arctic Ocean Arctique* Ellesmere Island Université Laval: CorpusUL Ace Lake ENVELOPE(78.188,78.188,-68.472,-68.472) Arctic Arctic Lake ENVELOPE(-130.826,-130.826,57.231,57.231) Arctic Ocean Ellesmere Island mSphere 5 3 |
institution |
Open Polar |
collection |
Université Laval: CorpusUL |
op_collection_id |
ftunivlavalcorp |
language |
English |
topic |
Aquatic viral ecology Limnology Polar science Viromics Virologie aquatique Limnologie Virus -- Écologie Glace |
spellingShingle |
Aquatic viral ecology Limnology Polar science Viromics Virologie aquatique Limnologie Virus -- Écologie Glace Culley, Alexander Girard, Catherine Vincent, Warwick F. Labbé, Myriam C. Extreme viral partitioning in a marine-derived high arctic lake |
topic_facet |
Aquatic viral ecology Limnology Polar science Viromics Virologie aquatique Limnologie Virus -- Écologie Glace |
description |
High-latitude, perennially stratified (meromictic) lakes are likely to be especially vulnerable to climate warming because of the importance of ice in maintaining their water column structure and associated distribution of microbial communities. This study aimed to characterize viral abundance, diversity, and distribution in a meromictic lake of marine origin on the far northern coast of Ellesmere Island, in the Canadian High Arctic. We collected triplicate samples for double-stranded DNA (dsDNA) viromics from five depths that encompassed the major features of the lake, as determined by limnological profiling of the water column. Viral abundance and virus-to-prokaryote ratios were highest at greater depths, while bacterial and cyanobacterial counts were greatest in the surface waters. The viral communities from each zone of the lake defined by salinity, temperature, and dissolved oxygen concentrations were markedly distinct, suggesting that there was little exchange of viral types among lake strata. Ten viral assembled genomes were obtained from our libraries, and these also segregated with depth. This well-defined structure of viral communities was consistent with that of potential hosts. Viruses from the monimolimnion, a deep layer of ancient Arctic Ocean seawater, were more diverse and relatively abundant, with few similarities to available viral sequences. The Lake A viral communities also differed from published records from the Arctic Ocean and meromictic Ace Lake in Antarctica. This first characterization of viral diversity from this sentinel environment underscores the microbial richness and complexity of an ecosystem type that is increasingly exposed to major perturbations in the fast-changing Arctic. |
format |
Other/Unknown Material |
author |
Culley, Alexander Girard, Catherine Vincent, Warwick F. Labbé, Myriam C. |
author_facet |
Culley, Alexander Girard, Catherine Vincent, Warwick F. Labbé, Myriam C. |
author_sort |
Culley, Alexander |
title |
Extreme viral partitioning in a marine-derived high arctic lake |
title_short |
Extreme viral partitioning in a marine-derived high arctic lake |
title_full |
Extreme viral partitioning in a marine-derived high arctic lake |
title_fullStr |
Extreme viral partitioning in a marine-derived high arctic lake |
title_full_unstemmed |
Extreme viral partitioning in a marine-derived high arctic lake |
title_sort |
extreme viral partitioning in a marine-derived high arctic lake |
publisher |
American Society for Microbiology |
publishDate |
2020 |
url |
https://hdl.handle.net/20.500.11794/39428 https://doi.org/10.1128/mSphere.00334-20 |
op_coverage |
Arctique Régions polaires |
long_lat |
ENVELOPE(78.188,78.188,-68.472,-68.472) ENVELOPE(-130.826,-130.826,57.231,57.231) |
geographic |
Ace Lake Arctic Arctic Lake Arctic Ocean Ellesmere Island |
geographic_facet |
Ace Lake Arctic Arctic Lake Arctic Ocean Ellesmere Island |
genre |
Antarc* Antarctica Arctic Arctic Ocean Arctique* Ellesmere Island |
genre_facet |
Antarc* Antarctica Arctic Arctic Ocean Arctique* Ellesmere Island |
op_relation |
2379-5042 http://hdl.handle.net/20.500.11794/39428 doi:10.1128/mSphere.00334-20 PMC7227771 |
op_rights |
http://purl.org/coar/access_right/c_abf2 |
op_doi |
https://doi.org/20.500.11794/3942810.1128/mSphere.00334-20 |
container_title |
mSphere |
container_volume |
5 |
container_issue |
3 |
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1802643841647378432 |