Metabolomic analysis of cold acclimation of arctic mesorhizobium sp. strain N33
Arctic Mesorhizobium sp. N33 isolated from nodules of Oxytropis arctobia in Canada’s eastern Arctic has a growth temperature range from 0°C to 30°C and is a well-known cold-adapted rhizobia. The key molecular mechanisms underlying cold adaptation in Arctic rhizobia remains totally unknown. Since the...
Published in: | PLoS ONE |
---|---|
Main Authors: | , , , , , , |
Format: | Other/Unknown Material |
Language: | English |
Published: |
Public Library of Science
2017
|
Subjects: | |
Online Access: | https://hdl.handle.net/20.500.11794/15735 https://doi.org/10.1371/journal.pone.0084801 |
id |
ftunivlavalcorp:oai:corpus.ulaval.ca:20.500.11794/15735 |
---|---|
record_format |
openpolar |
spelling |
ftunivlavalcorp:oai:corpus.ulaval.ca:20.500.11794/15735 2024-09-09T19:21:26+00:00 Metabolomic analysis of cold acclimation of arctic mesorhizobium sp. strain N33 Antoun, Hani Laberge, Serge Ghobakhlou, Abdollah Wishart, David Scott Xia, Jianguo Krishnamurthy, Ramanarayan Mandal, Rupasri Canada (Nord) Arctique 2017-10-26T13:58:48Z application/pdf https://hdl.handle.net/20.500.11794/15735 https://doi.org/10.1371/journal.pone.0084801 eng eng Public Library of Science 1932-6203 http://hdl.handle.net/20.500.11794/15735 doi:10.1371/journal.pone.0084801 3875568 http://purl.org/coar/access_right/c_abf2 Mesorhizobium sp. N33 Adaptation au froid Métabolomique article de recherche COAR1_1::Texte::Périodique::Revue::Contribution à un journal::Article::Article de recherche 2017 ftunivlavalcorp https://doi.org/20.500.11794/1573510.1371/journal.pone.0084801 2024-06-17T23:42:35Z Arctic Mesorhizobium sp. N33 isolated from nodules of Oxytropis arctobia in Canada’s eastern Arctic has a growth temperature range from 0°C to 30°C and is a well-known cold-adapted rhizobia. The key molecular mechanisms underlying cold adaptation in Arctic rhizobia remains totally unknown. Since the concentration and contents of metabolites are closely related to stress adaptation, we applied GC-MS and NMR to identify and quantify fatty acids and water soluble compounds possibly related to low temperature acclimation in strain N33. Bacterial cells were grown at three different growing temperatures (4°C, 10°C and 21°C). Cells from 21°C were also cold-exposed to 4°C for different times (2, 4, 8, 60 and 240 minutes). We identified that poly-unsaturated linoleic acids 18∶2 (9, 12) & 18∶2 (6, 9) were more abundant in cells growing at 4 or 10°C, than in cells cultivated at 21°C. The mono-unsaturated phospho/neutral fatty acids myristoleic acid 14∶1(11) were the most significantly overexpressed (45-fold) after 1hour of exposure to 4°C. As reported in the literature, these fatty acids play important roles in cold adaptability by supplying cell membrane fluidity, and by providing energy to cells. Analysis of water-soluble compounds revealed that isobutyrate, sarcosine, threonine and valine were more accumulated during exposure to 4°C. These metabolites might play a role in conferring cold acclimation to strain N33 at 4°C, probably by acting as cryoprotectants. Isobutyrate was highly upregulated (19.4-fold) during growth at 4°C, thus suggesting that this compound is a precursor for the cold-regulated fatty acids modification to low temperature adaptation. Other/Unknown Material Arctic Arctique* Université Laval: CorpusUL Arctic Canada PLoS ONE 8 12 e84801 |
institution |
Open Polar |
collection |
Université Laval: CorpusUL |
op_collection_id |
ftunivlavalcorp |
language |
English |
topic |
Mesorhizobium sp. N33 Adaptation au froid Métabolomique |
spellingShingle |
Mesorhizobium sp. N33 Adaptation au froid Métabolomique Antoun, Hani Laberge, Serge Ghobakhlou, Abdollah Wishart, David Scott Xia, Jianguo Krishnamurthy, Ramanarayan Mandal, Rupasri Metabolomic analysis of cold acclimation of arctic mesorhizobium sp. strain N33 |
topic_facet |
Mesorhizobium sp. N33 Adaptation au froid Métabolomique |
description |
Arctic Mesorhizobium sp. N33 isolated from nodules of Oxytropis arctobia in Canada’s eastern Arctic has a growth temperature range from 0°C to 30°C and is a well-known cold-adapted rhizobia. The key molecular mechanisms underlying cold adaptation in Arctic rhizobia remains totally unknown. Since the concentration and contents of metabolites are closely related to stress adaptation, we applied GC-MS and NMR to identify and quantify fatty acids and water soluble compounds possibly related to low temperature acclimation in strain N33. Bacterial cells were grown at three different growing temperatures (4°C, 10°C and 21°C). Cells from 21°C were also cold-exposed to 4°C for different times (2, 4, 8, 60 and 240 minutes). We identified that poly-unsaturated linoleic acids 18∶2 (9, 12) & 18∶2 (6, 9) were more abundant in cells growing at 4 or 10°C, than in cells cultivated at 21°C. The mono-unsaturated phospho/neutral fatty acids myristoleic acid 14∶1(11) were the most significantly overexpressed (45-fold) after 1hour of exposure to 4°C. As reported in the literature, these fatty acids play important roles in cold adaptability by supplying cell membrane fluidity, and by providing energy to cells. Analysis of water-soluble compounds revealed that isobutyrate, sarcosine, threonine and valine were more accumulated during exposure to 4°C. These metabolites might play a role in conferring cold acclimation to strain N33 at 4°C, probably by acting as cryoprotectants. Isobutyrate was highly upregulated (19.4-fold) during growth at 4°C, thus suggesting that this compound is a precursor for the cold-regulated fatty acids modification to low temperature adaptation. |
format |
Other/Unknown Material |
author |
Antoun, Hani Laberge, Serge Ghobakhlou, Abdollah Wishart, David Scott Xia, Jianguo Krishnamurthy, Ramanarayan Mandal, Rupasri |
author_facet |
Antoun, Hani Laberge, Serge Ghobakhlou, Abdollah Wishart, David Scott Xia, Jianguo Krishnamurthy, Ramanarayan Mandal, Rupasri |
author_sort |
Antoun, Hani |
title |
Metabolomic analysis of cold acclimation of arctic mesorhizobium sp. strain N33 |
title_short |
Metabolomic analysis of cold acclimation of arctic mesorhizobium sp. strain N33 |
title_full |
Metabolomic analysis of cold acclimation of arctic mesorhizobium sp. strain N33 |
title_fullStr |
Metabolomic analysis of cold acclimation of arctic mesorhizobium sp. strain N33 |
title_full_unstemmed |
Metabolomic analysis of cold acclimation of arctic mesorhizobium sp. strain N33 |
title_sort |
metabolomic analysis of cold acclimation of arctic mesorhizobium sp. strain n33 |
publisher |
Public Library of Science |
publishDate |
2017 |
url |
https://hdl.handle.net/20.500.11794/15735 https://doi.org/10.1371/journal.pone.0084801 |
op_coverage |
Canada (Nord) Arctique |
geographic |
Arctic Canada |
geographic_facet |
Arctic Canada |
genre |
Arctic Arctique* |
genre_facet |
Arctic Arctique* |
op_relation |
1932-6203 http://hdl.handle.net/20.500.11794/15735 doi:10.1371/journal.pone.0084801 3875568 |
op_rights |
http://purl.org/coar/access_right/c_abf2 |
op_doi |
https://doi.org/20.500.11794/1573510.1371/journal.pone.0084801 |
container_title |
PLoS ONE |
container_volume |
8 |
container_issue |
12 |
container_start_page |
e84801 |
_version_ |
1809761627468201984 |