Microarray transcriptional profiling of Arctic Mesorhizobium strain N33 at low temperature provides insights into cold adaption strategies
Background Arctic Mesorhizobium strain N33 was isolated from nodules of the legume Oxytropis arctobia in Canada’s eastern Arctic. This symbiotic bacterium can grow at temperatures ranging from 0 to 30 °C, fix nitrogen at 10 °C, and is one of the best known cold-adapted rhizobia. Despite the economic...
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ftunivlavalcorp:oai:corpus.ulaval.ca:20.500.11794/15734 2024-06-23T07:49:41+00:00 Microarray transcriptional profiling of Arctic Mesorhizobium strain N33 at low temperature provides insights into cold adaption strategies Antoun, Hani Klein, Anne Laberge, Serge Ghobakhlou, Abdollah Canada (Nord) Arctique 2017-10-26T13:51:25Z application/pdf https://hdl.handle.net/20.500.11794/15734 https://doi.org/10.1186/s12864-015-1611-4 eng eng BioMed Central 14712164 http://hdl.handle.net/20.500.11794/15734 doi:10.1186/s12864-015-1611-4 4432818 http://purl.org/coar/access_right/c_abf2 α-proteobacteria Genomic library Microarray Gene expression Transcriptomics Quantitative PCR Cold adaptation Nitrogen fixation Arctic Mesorhizobium Puces à protéines Transcriptomes Mesorhizobium sp. N33 Adaptation au froid Expression génique Azote -- Fixation article de recherche COAR1_1::Texte::Périodique::Revue::Contribution à un journal::Article::Article de recherche 2017 ftunivlavalcorp https://doi.org/20.500.11794/1573410.1186/s12864-015-1611-4 2024-06-10T23:42:53Z Background Arctic Mesorhizobium strain N33 was isolated from nodules of the legume Oxytropis arctobia in Canada’s eastern Arctic. This symbiotic bacterium can grow at temperatures ranging from 0 to 30 °C, fix nitrogen at 10 °C, and is one of the best known cold-adapted rhizobia. Despite the economic potential of this bacterium for northern regions, the key molecular mechanisms of its cold adaptation remain poorly understood. Results Using a microarray printed with 5760 Arctic Mesorhizobium genomic clones, we performed a partial transcriptome analysis of strain N33 grown under eight different temperature conditions, including both sustained and transient cold treatments, compared with cells grown at room temperature. Cells treated under constant (4 and 10 °C) low temperatures expressed a prominent number of induced genes distinct from cells treated to short-term cold-exposure (<60 min), but exhibited an intermediate expression profile when exposed to a prolonged cold exposure (240 min). The most prominent up-regulated genes encode proteins involved in metabolite transport, transcription regulation, protein turnover, oxidoreductase activity, cryoprotection (mannitol, polyamines), fatty acid metabolism, and membrane fluidity. The main categories of genes affected in N33 during cold treatment are sugar transport and protein translocation, lipid biosynthesis, and NADH oxidoreductase (quinone) activity. Some genes were significantly down-regulated and classified in secretion, energy production and conversion, amino acid transport, cell motility, cell envelope and outer membrane biogenesis functions. This might suggest growth cessation or reduction, which is an important strategy to adjust cellular function and save energy under cold stress conditions. Conclusion Our analysis revealed a complex series of changes associated with cold exposure adaptation and constant growth at low temperatures. Moreover, it highlighted some of the strategies and different physiological states that Mesorhizobium strain N33 has ... Other/Unknown Material Arctic Arctique* Université Laval: CorpusUL Arctic Canada BMC Genomics 16 1 |
institution |
Open Polar |
collection |
Université Laval: CorpusUL |
op_collection_id |
ftunivlavalcorp |
language |
English |
topic |
α-proteobacteria Genomic library Microarray Gene expression Transcriptomics Quantitative PCR Cold adaptation Nitrogen fixation Arctic Mesorhizobium Puces à protéines Transcriptomes Mesorhizobium sp. N33 Adaptation au froid Expression génique Azote -- Fixation |
spellingShingle |
α-proteobacteria Genomic library Microarray Gene expression Transcriptomics Quantitative PCR Cold adaptation Nitrogen fixation Arctic Mesorhizobium Puces à protéines Transcriptomes Mesorhizobium sp. N33 Adaptation au froid Expression génique Azote -- Fixation Antoun, Hani Klein, Anne Laberge, Serge Ghobakhlou, Abdollah Microarray transcriptional profiling of Arctic Mesorhizobium strain N33 at low temperature provides insights into cold adaption strategies |
topic_facet |
α-proteobacteria Genomic library Microarray Gene expression Transcriptomics Quantitative PCR Cold adaptation Nitrogen fixation Arctic Mesorhizobium Puces à protéines Transcriptomes Mesorhizobium sp. N33 Adaptation au froid Expression génique Azote -- Fixation |
description |
Background Arctic Mesorhizobium strain N33 was isolated from nodules of the legume Oxytropis arctobia in Canada’s eastern Arctic. This symbiotic bacterium can grow at temperatures ranging from 0 to 30 °C, fix nitrogen at 10 °C, and is one of the best known cold-adapted rhizobia. Despite the economic potential of this bacterium for northern regions, the key molecular mechanisms of its cold adaptation remain poorly understood. Results Using a microarray printed with 5760 Arctic Mesorhizobium genomic clones, we performed a partial transcriptome analysis of strain N33 grown under eight different temperature conditions, including both sustained and transient cold treatments, compared with cells grown at room temperature. Cells treated under constant (4 and 10 °C) low temperatures expressed a prominent number of induced genes distinct from cells treated to short-term cold-exposure (<60 min), but exhibited an intermediate expression profile when exposed to a prolonged cold exposure (240 min). The most prominent up-regulated genes encode proteins involved in metabolite transport, transcription regulation, protein turnover, oxidoreductase activity, cryoprotection (mannitol, polyamines), fatty acid metabolism, and membrane fluidity. The main categories of genes affected in N33 during cold treatment are sugar transport and protein translocation, lipid biosynthesis, and NADH oxidoreductase (quinone) activity. Some genes were significantly down-regulated and classified in secretion, energy production and conversion, amino acid transport, cell motility, cell envelope and outer membrane biogenesis functions. This might suggest growth cessation or reduction, which is an important strategy to adjust cellular function and save energy under cold stress conditions. Conclusion Our analysis revealed a complex series of changes associated with cold exposure adaptation and constant growth at low temperatures. Moreover, it highlighted some of the strategies and different physiological states that Mesorhizobium strain N33 has ... |
format |
Other/Unknown Material |
author |
Antoun, Hani Klein, Anne Laberge, Serge Ghobakhlou, Abdollah |
author_facet |
Antoun, Hani Klein, Anne Laberge, Serge Ghobakhlou, Abdollah |
author_sort |
Antoun, Hani |
title |
Microarray transcriptional profiling of Arctic Mesorhizobium strain N33 at low temperature provides insights into cold adaption strategies |
title_short |
Microarray transcriptional profiling of Arctic Mesorhizobium strain N33 at low temperature provides insights into cold adaption strategies |
title_full |
Microarray transcriptional profiling of Arctic Mesorhizobium strain N33 at low temperature provides insights into cold adaption strategies |
title_fullStr |
Microarray transcriptional profiling of Arctic Mesorhizobium strain N33 at low temperature provides insights into cold adaption strategies |
title_full_unstemmed |
Microarray transcriptional profiling of Arctic Mesorhizobium strain N33 at low temperature provides insights into cold adaption strategies |
title_sort |
microarray transcriptional profiling of arctic mesorhizobium strain n33 at low temperature provides insights into cold adaption strategies |
publisher |
BioMed Central |
publishDate |
2017 |
url |
https://hdl.handle.net/20.500.11794/15734 https://doi.org/10.1186/s12864-015-1611-4 |
op_coverage |
Canada (Nord) Arctique |
geographic |
Arctic Canada |
geographic_facet |
Arctic Canada |
genre |
Arctic Arctique* |
genre_facet |
Arctic Arctique* |
op_relation |
14712164 http://hdl.handle.net/20.500.11794/15734 doi:10.1186/s12864-015-1611-4 4432818 |
op_rights |
http://purl.org/coar/access_right/c_abf2 |
op_doi |
https://doi.org/20.500.11794/1573410.1186/s12864-015-1611-4 |
container_title |
BMC Genomics |
container_volume |
16 |
container_issue |
1 |
_version_ |
1802640267408310272 |