Dominance of unicellular cyanobacteria in the diazotrophic community in the Atlantic Ocean
The horizontal and vertical distribution of representatives of diazotrophic unicellular cyanobacteria wasinvestigated in the subtropical northeast Atlantic Ocean (28.87 to 42.00uN; 9.01 to 20.02uW). Samples fromstations encompassing different water conditions (from oceanic oligotrophic waters to upw...
Published in: | Limnology and Oceanography |
---|---|
Main Authors: | , , , , , |
Other Authors: | , , , , , , , , , , , , , , |
Language: | English |
Published: |
0024-3590
2014
|
Subjects: | |
Online Access: | http://hdl.handle.net/10553/46907 https://doi.org/10.4319/lo.2014.59.2.0623 |
id |
ftunivlaspalmas:oai:https://accedacris.ulpgc.es:10553/46907 |
---|---|
record_format |
openpolar |
spelling |
ftunivlaspalmas:oai:https://accedacris.ulpgc.es:10553/46907 2023-05-15T17:41:31+02:00 Dominance of unicellular cyanobacteria in the diazotrophic community in the Atlantic Ocean Agawin, Nona S.R. Benavides Gorostegui,Mar Busquets, Antonio Ferriol, Pere Stal, Lucas J. Aristegui, Javier Benavides, Mar Agawin, Nona Sheila Aristegui, Javier Ferriol, Pere Busquets Bisbal, Antonio 6603773899 36501571300 6603551155 56063231200 7004241609 7006816204 1073894 2027943 2636444 4812359 159788 227201 2014 http://hdl.handle.net/10553/46907 https://doi.org/10.4319/lo.2014.59.2.0623 eng eng 0024-3590 Intercambio Plataforma-Oceano en El Ecosistema Marino de Las Islas Canarias-Peninsula Iberica (Caibex):Afloramiento de Cabo Guir Limnology and Oceanography 0024-3590 http://hdl.handle.net/10553/46907 doi:10.4319/lo.2014.59.2.0623 2-s2.0-84895856360 000339902800024 637 623 59 WOS:000339902800024 K-5323-2015 I-3168-2015 D-5833-2013 No ID No ID WOS:000339902800024 Limnology And Oceanography [ISSN 0024-3590], v. 59 (2), p. 623-637 251001 Oceanografía biológica Cyanobacteria Atlantic Ocean 2014 ftunivlaspalmas https://doi.org/10.4319/lo.2014.59.2.0623 2019-11-27T00:06:56Z The horizontal and vertical distribution of representatives of diazotrophic unicellular cyanobacteria wasinvestigated in the subtropical northeast Atlantic Ocean (28.87 to 42.00uN; 9.01 to 20.02uW). Samples fromstations encompassing different water conditions (from oceanic oligotrophic waters to upwelling areas and atemperature range of 13.1uC to 24.2uC) were size fractionated and analyzed fornifHby a nested polymerase chainreaction (PCR) and by tyramide signal amplification–fluorescence in situ hybridization (TSA-FISH) using probeNitro821. In samples from the surface, mixed-layer depth, and deep chlorophyll maximum waters, most (.50%)of thenifHrecovered was from the 0.2–3mm fraction and was consistent with TSA-FISH counts. The,3mmNitro821-positive cells were more abundant than the larger cells, and the proportion of single cells was larger thanthat associated with particulate matter or with larger cells. Phylogenetic analysis of representative samplesrevealed that most of the sequences belong to diazotrophic unicellular cyanobacteria Group A (UCYN-A orCandidatusAtelocyanobacterium thalassa). N2fixation in the 0.2–3mm fraction, putatively representing theactivity of UCYN-A, contributed more than 50%of the total N2fixation. There was a positive relationship of thisputative UCYN-A abundance and activity with temperature, and a negative relationship with dissolved O2. Thedominance of these putative UCYN-A organisms in nitrate-rich upwelling filament regions suggests that theactivity of this group of organisms may not be strongly controlled by the availability of fixed N. Other/Unknown Material Northeast Atlantic Universidad de Las Palmas de Gran Canaria: Acceda Limnology and Oceanography 59 2 623 637 |
institution |
Open Polar |
collection |
Universidad de Las Palmas de Gran Canaria: Acceda |
op_collection_id |
ftunivlaspalmas |
language |
English |
topic |
251001 Oceanografía biológica Cyanobacteria Atlantic Ocean |
spellingShingle |
251001 Oceanografía biológica Cyanobacteria Atlantic Ocean Agawin, Nona S.R. Benavides Gorostegui,Mar Busquets, Antonio Ferriol, Pere Stal, Lucas J. Aristegui, Javier Dominance of unicellular cyanobacteria in the diazotrophic community in the Atlantic Ocean |
topic_facet |
251001 Oceanografía biológica Cyanobacteria Atlantic Ocean |
description |
The horizontal and vertical distribution of representatives of diazotrophic unicellular cyanobacteria wasinvestigated in the subtropical northeast Atlantic Ocean (28.87 to 42.00uN; 9.01 to 20.02uW). Samples fromstations encompassing different water conditions (from oceanic oligotrophic waters to upwelling areas and atemperature range of 13.1uC to 24.2uC) were size fractionated and analyzed fornifHby a nested polymerase chainreaction (PCR) and by tyramide signal amplification–fluorescence in situ hybridization (TSA-FISH) using probeNitro821. In samples from the surface, mixed-layer depth, and deep chlorophyll maximum waters, most (.50%)of thenifHrecovered was from the 0.2–3mm fraction and was consistent with TSA-FISH counts. The,3mmNitro821-positive cells were more abundant than the larger cells, and the proportion of single cells was larger thanthat associated with particulate matter or with larger cells. Phylogenetic analysis of representative samplesrevealed that most of the sequences belong to diazotrophic unicellular cyanobacteria Group A (UCYN-A orCandidatusAtelocyanobacterium thalassa). N2fixation in the 0.2–3mm fraction, putatively representing theactivity of UCYN-A, contributed more than 50%of the total N2fixation. There was a positive relationship of thisputative UCYN-A abundance and activity with temperature, and a negative relationship with dissolved O2. Thedominance of these putative UCYN-A organisms in nitrate-rich upwelling filament regions suggests that theactivity of this group of organisms may not be strongly controlled by the availability of fixed N. |
author2 |
Benavides, Mar Agawin, Nona Sheila Aristegui, Javier Ferriol, Pere Busquets Bisbal, Antonio 6603773899 36501571300 6603551155 56063231200 7004241609 7006816204 1073894 2027943 2636444 4812359 159788 227201 |
author |
Agawin, Nona S.R. Benavides Gorostegui,Mar Busquets, Antonio Ferriol, Pere Stal, Lucas J. Aristegui, Javier |
author_facet |
Agawin, Nona S.R. Benavides Gorostegui,Mar Busquets, Antonio Ferriol, Pere Stal, Lucas J. Aristegui, Javier |
author_sort |
Agawin, Nona S.R. |
title |
Dominance of unicellular cyanobacteria in the diazotrophic community in the Atlantic Ocean |
title_short |
Dominance of unicellular cyanobacteria in the diazotrophic community in the Atlantic Ocean |
title_full |
Dominance of unicellular cyanobacteria in the diazotrophic community in the Atlantic Ocean |
title_fullStr |
Dominance of unicellular cyanobacteria in the diazotrophic community in the Atlantic Ocean |
title_full_unstemmed |
Dominance of unicellular cyanobacteria in the diazotrophic community in the Atlantic Ocean |
title_sort |
dominance of unicellular cyanobacteria in the diazotrophic community in the atlantic ocean |
publisher |
0024-3590 |
publishDate |
2014 |
url |
http://hdl.handle.net/10553/46907 https://doi.org/10.4319/lo.2014.59.2.0623 |
genre |
Northeast Atlantic |
genre_facet |
Northeast Atlantic |
op_source |
Limnology And Oceanography [ISSN 0024-3590], v. 59 (2), p. 623-637 |
op_relation |
Intercambio Plataforma-Oceano en El Ecosistema Marino de Las Islas Canarias-Peninsula Iberica (Caibex):Afloramiento de Cabo Guir Limnology and Oceanography 0024-3590 http://hdl.handle.net/10553/46907 doi:10.4319/lo.2014.59.2.0623 2-s2.0-84895856360 000339902800024 637 623 59 WOS:000339902800024 K-5323-2015 I-3168-2015 D-5833-2013 No ID No ID WOS:000339902800024 |
op_doi |
https://doi.org/10.4319/lo.2014.59.2.0623 |
container_title |
Limnology and Oceanography |
container_volume |
59 |
container_issue |
2 |
container_start_page |
623 |
op_container_end_page |
637 |
_version_ |
1766143121036410880 |