INT Toxicity over Natural Bacterial Assemblages from Surface Oligotrophic Waters: Implications for the Assessment of Respiratory Activity
Plankton community respiration (R) is a major component of the carbon flux in aquatic ecosystems. However, current methods to measure actual respiration from oxygen consumption at relevant spatial scales are not sensitive enough in oligotrophic environments where respiration rates are very low. To o...
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Online Access: | http://hdl.handle.net/10553/60284 https://doi.org/10.1007/s00248-019-01479-4 |
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ftunivlaspalmas:oai:accedacris.ulpgc.es:10553/60284 2023-05-15T17:35:42+02:00 INT Toxicity over Natural Bacterial Assemblages from Surface Oligotrophic Waters: Implications for the Assessment of Respiratory Activity Baños, Isabel Montero, María F. Benavides Gorostegui,Mar Arístegui, Javier 57190220268 7102553402 36501571300 7006816204 BU-BAS 2020 http://hdl.handle.net/10553/60284 https://doi.org/10.1007/s00248-019-01479-4 eng eng BES-2016- 078407 INNOVA Canarias 2020 Flujos de Carbono en Un Sistema de Afloramiento Costero (Cabo Blanco, Nw de África); Modulación A Submesoscala de la Producción, Exportación y Consumo de Carbono Microbial Ecology 0095-3628 Scopus http://hdl.handle.net/10553/60284 doi:10.1007/s00248-019-01479-4 85077604208 Sí Microbial Ecology [ISSN 0095-3628], v. 80, p. 237–242 251001 Oceanografía biológica ETS Bacterioplankton Cell viability Leucine incorporation Bacterial respiration info:eu-repo/semantics/article Article 2020 ftunivlaspalmas https://doi.org/10.1007/s00248-019-01479-4 2022-05-17T23:13:54Z Plankton community respiration (R) is a major component of the carbon flux in aquatic ecosystems. However, current methods to measure actual respiration from oxygen consumption at relevant spatial scales are not sensitive enough in oligotrophic environments where respiration rates are very low. To overcome this drawback, more sensitive indirect enzymatic approaches are commonly used as R proxies. The in vivo electron transport system (ETSvivo) assay, which measures the reduction of (2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyl tetrazolium chloride salt, INT) to INT-formazan in the presence of natural substrate levels, was recently proposed as an indirect reliable estimation of R for natural plankton communities. However, under in vivo conditions, formazan salts could be toxic to the cells. Here, we test the toxicity of 0.2 mM of final INT concentration, widely used for ETSvivo assays, on natural bacterial assemblages collected in coastal and oceanic waters off Gran Canaria (Canary Islands, subtropical North Atlantic), in eight independent experiments. After 0.5 h of incubation, a significant but variable decline in cell viability (14–49%) was observed in all samples inoculated with INT. Moreover, INT also inhibited leucine uptake in less than 90 min of incubation. In the light of these results, we argue that enzymatic respiratory rates obtained with the ETSvivo method need to be interpreted with caution to derive R in oceanic regions where bacteria largely contribute to community respiration. Moreover, the variable toxicity on bacterial assemblages observed in our experiments questions the use of a single R/ETSvivo relationship as a universal proxy for regional studies. 1,161 4,552 Q1 Q1 SCIE Article in Journal/Newspaper North Atlantic Universidad de Las Palmas de Gran Canaria: Acceda Microbial Ecology 80 1 237 242 |
institution |
Open Polar |
collection |
Universidad de Las Palmas de Gran Canaria: Acceda |
op_collection_id |
ftunivlaspalmas |
language |
English |
topic |
251001 Oceanografía biológica ETS Bacterioplankton Cell viability Leucine incorporation Bacterial respiration |
spellingShingle |
251001 Oceanografía biológica ETS Bacterioplankton Cell viability Leucine incorporation Bacterial respiration Baños, Isabel Montero, María F. Benavides Gorostegui,Mar Arístegui, Javier INT Toxicity over Natural Bacterial Assemblages from Surface Oligotrophic Waters: Implications for the Assessment of Respiratory Activity |
topic_facet |
251001 Oceanografía biológica ETS Bacterioplankton Cell viability Leucine incorporation Bacterial respiration |
description |
Plankton community respiration (R) is a major component of the carbon flux in aquatic ecosystems. However, current methods to measure actual respiration from oxygen consumption at relevant spatial scales are not sensitive enough in oligotrophic environments where respiration rates are very low. To overcome this drawback, more sensitive indirect enzymatic approaches are commonly used as R proxies. The in vivo electron transport system (ETSvivo) assay, which measures the reduction of (2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyl tetrazolium chloride salt, INT) to INT-formazan in the presence of natural substrate levels, was recently proposed as an indirect reliable estimation of R for natural plankton communities. However, under in vivo conditions, formazan salts could be toxic to the cells. Here, we test the toxicity of 0.2 mM of final INT concentration, widely used for ETSvivo assays, on natural bacterial assemblages collected in coastal and oceanic waters off Gran Canaria (Canary Islands, subtropical North Atlantic), in eight independent experiments. After 0.5 h of incubation, a significant but variable decline in cell viability (14–49%) was observed in all samples inoculated with INT. Moreover, INT also inhibited leucine uptake in less than 90 min of incubation. In the light of these results, we argue that enzymatic respiratory rates obtained with the ETSvivo method need to be interpreted with caution to derive R in oceanic regions where bacteria largely contribute to community respiration. Moreover, the variable toxicity on bacterial assemblages observed in our experiments questions the use of a single R/ETSvivo relationship as a universal proxy for regional studies. 1,161 4,552 Q1 Q1 SCIE |
author2 |
57190220268 7102553402 36501571300 7006816204 BU-BAS |
format |
Article in Journal/Newspaper |
author |
Baños, Isabel Montero, María F. Benavides Gorostegui,Mar Arístegui, Javier |
author_facet |
Baños, Isabel Montero, María F. Benavides Gorostegui,Mar Arístegui, Javier |
author_sort |
Baños, Isabel |
title |
INT Toxicity over Natural Bacterial Assemblages from Surface Oligotrophic Waters: Implications for the Assessment of Respiratory Activity |
title_short |
INT Toxicity over Natural Bacterial Assemblages from Surface Oligotrophic Waters: Implications for the Assessment of Respiratory Activity |
title_full |
INT Toxicity over Natural Bacterial Assemblages from Surface Oligotrophic Waters: Implications for the Assessment of Respiratory Activity |
title_fullStr |
INT Toxicity over Natural Bacterial Assemblages from Surface Oligotrophic Waters: Implications for the Assessment of Respiratory Activity |
title_full_unstemmed |
INT Toxicity over Natural Bacterial Assemblages from Surface Oligotrophic Waters: Implications for the Assessment of Respiratory Activity |
title_sort |
int toxicity over natural bacterial assemblages from surface oligotrophic waters: implications for the assessment of respiratory activity |
publishDate |
2020 |
url |
http://hdl.handle.net/10553/60284 https://doi.org/10.1007/s00248-019-01479-4 |
genre |
North Atlantic |
genre_facet |
North Atlantic |
op_source |
Microbial Ecology [ISSN 0095-3628], v. 80, p. 237–242 |
op_relation |
BES-2016- 078407 INNOVA Canarias 2020 Flujos de Carbono en Un Sistema de Afloramiento Costero (Cabo Blanco, Nw de África); Modulación A Submesoscala de la Producción, Exportación y Consumo de Carbono Microbial Ecology 0095-3628 Scopus http://hdl.handle.net/10553/60284 doi:10.1007/s00248-019-01479-4 85077604208 Sí |
op_doi |
https://doi.org/10.1007/s00248-019-01479-4 |
container_title |
Microbial Ecology |
container_volume |
80 |
container_issue |
1 |
container_start_page |
237 |
op_container_end_page |
242 |
_version_ |
1766134955078844416 |