Development of two new microsatellite multiplex PCRs for three sparid species: Gilthead seabream (Spares auratus L.), red porgy (Pagrus pagrus L.) and redbanded seabream (P. auriga, Valenciennes, 1843) and their application to paternity studies
This study reports two new and highly informative multiplex PCRs of microsatellite markers, with redesigned interspecific primer sets for three sparid species: gilthead seabream (Spates auratus L), red porgy (Pagrus pagrus L) and redbanded seabream (P auriga, Valenciennes, 1843). The evaluation and...
Published in: | Aquaculture |
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Format: | Article in Journal/Newspaper |
Language: | unknown |
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0044-8486
2008
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Online Access: | http://hdl.handle.net/10553/53105 https://doi.org/10.1016/j.aquaculture.2008.07.005 |
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ftunivlaspalmas:oai:accedacris.ulpgc.es:10553/53105 2023-05-15T18:09:59+02:00 Development of two new microsatellite multiplex PCRs for three sparid species: Gilthead seabream (Spares auratus L.), red porgy (Pagrus pagrus L.) and redbanded seabream (P. auriga, Valenciennes, 1843) and their application to paternity studies Navarro, A. Badilla, R. Zamorano, M. J. Pasamontes, V. Hildebrandt, S. Sanchez, J. J. Afonso, J. M. Zamorano, Maria Jesus Hildebrandt, Silvia 2095438 8070891 1009633 17402957 33416633 7765687 8078817 1136306 WOS:Navarro, A WOS:Badilla, R WOS:Zamorano, MJ WOS:Pasamontes, V WOS:Hildebrandt, S WOS:Sanchez, JJ WOS:Afonso, JM 2008 http://hdl.handle.net/10553/53105 https://doi.org/10.1016/j.aquaculture.2008.07.005 unknown 0044-8486 Aquaculture 285 0044-8486 http://hdl.handle.net/10553/53105 doi:10.1016/j.aquaculture.2008.07.005 000261729200006 1-4 WOS:000261729200006 M-5052-2018 E-3879-2010 Sí Aquaculture[ISSN 0044-8486],v. 285 (1-4), p. 30-37 Salmo-Salar L Parentage Loci Atlantic Markers Fish Amplification Populations Assignment System info:eu-repo/semantics/Article Article 2008 ftunivlaspalmas https://doi.org/10.1016/j.aquaculture.2008.07.005 2021-05-04T23:10:20Z This study reports two new and highly informative multiplex PCRs of microsatellite markers, with redesigned interspecific primer sets for three sparid species: gilthead seabream (Spates auratus L), red porgy (Pagrus pagrus L) and redbanded seabream (P auriga, Valenciennes, 1843). The evaluation and validation of the two multiplex PCRs, named RimA and RimB (Redesigned Interspecific Multiplex), were carried out upon: 148 individual gilthead seabream (66 breeders of unknown gender, and 82 of their descendents obtained by mass-spawning), 37 red porgy and 125 redbanded seabream. From 15 and 12 redesigned microsatellite markers for RimA and RimB, respectively. the number of markers included in the final multiplex PCRs were 10 in RimA and 7 in RimB for gilthead seabream, 6 in each multiplex PCR for red porgy and 8 in RimA and 5 in RimB for redbanded seabream. The a priori combined parental exclusion probability for each multiplex in the three species was 0.999. For gilthead seabream, it was possible to assign each offspring to a single parent pair (100% success) using the exclusion method with at least seven microsatellite markers for each multiplex PCR. Null alleles were found only for marker PbMS2, through familial segregation, with a frequency similar to the expected one (0.09 vs 0.14). Results revealed that the multiplex reaction no more than one-sixth the cost of single reactions even when these reactions were performed in a unique run, and that genotyping errors were minimized due to automation. These robust multiplex PCRs will be a fundamental tool for the industry to introduce selection programs and to manage their broodstocks under industrial conditions. (C) 2008 Elsevier B.V. All rights reserved. 37 30 Article in Journal/Newspaper Salmo salar Universidad de Las Palmas de Gran Canaria: Acceda Rima ENVELOPE(-45.400,-45.400,-60.633,-60.633) Aquaculture 285 1-4 30 37 |
institution |
Open Polar |
collection |
Universidad de Las Palmas de Gran Canaria: Acceda |
op_collection_id |
ftunivlaspalmas |
language |
unknown |
topic |
Salmo-Salar L Parentage Loci Atlantic Markers Fish Amplification Populations Assignment System |
spellingShingle |
Salmo-Salar L Parentage Loci Atlantic Markers Fish Amplification Populations Assignment System Navarro, A. Badilla, R. Zamorano, M. J. Pasamontes, V. Hildebrandt, S. Sanchez, J. J. Afonso, J. M. Development of two new microsatellite multiplex PCRs for three sparid species: Gilthead seabream (Spares auratus L.), red porgy (Pagrus pagrus L.) and redbanded seabream (P. auriga, Valenciennes, 1843) and their application to paternity studies |
topic_facet |
Salmo-Salar L Parentage Loci Atlantic Markers Fish Amplification Populations Assignment System |
description |
This study reports two new and highly informative multiplex PCRs of microsatellite markers, with redesigned interspecific primer sets for three sparid species: gilthead seabream (Spates auratus L), red porgy (Pagrus pagrus L) and redbanded seabream (P auriga, Valenciennes, 1843). The evaluation and validation of the two multiplex PCRs, named RimA and RimB (Redesigned Interspecific Multiplex), were carried out upon: 148 individual gilthead seabream (66 breeders of unknown gender, and 82 of their descendents obtained by mass-spawning), 37 red porgy and 125 redbanded seabream. From 15 and 12 redesigned microsatellite markers for RimA and RimB, respectively. the number of markers included in the final multiplex PCRs were 10 in RimA and 7 in RimB for gilthead seabream, 6 in each multiplex PCR for red porgy and 8 in RimA and 5 in RimB for redbanded seabream. The a priori combined parental exclusion probability for each multiplex in the three species was 0.999. For gilthead seabream, it was possible to assign each offspring to a single parent pair (100% success) using the exclusion method with at least seven microsatellite markers for each multiplex PCR. Null alleles were found only for marker PbMS2, through familial segregation, with a frequency similar to the expected one (0.09 vs 0.14). Results revealed that the multiplex reaction no more than one-sixth the cost of single reactions even when these reactions were performed in a unique run, and that genotyping errors were minimized due to automation. These robust multiplex PCRs will be a fundamental tool for the industry to introduce selection programs and to manage their broodstocks under industrial conditions. (C) 2008 Elsevier B.V. All rights reserved. 37 30 |
author2 |
Zamorano, Maria Jesus Hildebrandt, Silvia 2095438 8070891 1009633 17402957 33416633 7765687 8078817 1136306 WOS:Navarro, A WOS:Badilla, R WOS:Zamorano, MJ WOS:Pasamontes, V WOS:Hildebrandt, S WOS:Sanchez, JJ WOS:Afonso, JM |
format |
Article in Journal/Newspaper |
author |
Navarro, A. Badilla, R. Zamorano, M. J. Pasamontes, V. Hildebrandt, S. Sanchez, J. J. Afonso, J. M. |
author_facet |
Navarro, A. Badilla, R. Zamorano, M. J. Pasamontes, V. Hildebrandt, S. Sanchez, J. J. Afonso, J. M. |
author_sort |
Navarro, A. |
title |
Development of two new microsatellite multiplex PCRs for three sparid species: Gilthead seabream (Spares auratus L.), red porgy (Pagrus pagrus L.) and redbanded seabream (P. auriga, Valenciennes, 1843) and their application to paternity studies |
title_short |
Development of two new microsatellite multiplex PCRs for three sparid species: Gilthead seabream (Spares auratus L.), red porgy (Pagrus pagrus L.) and redbanded seabream (P. auriga, Valenciennes, 1843) and their application to paternity studies |
title_full |
Development of two new microsatellite multiplex PCRs for three sparid species: Gilthead seabream (Spares auratus L.), red porgy (Pagrus pagrus L.) and redbanded seabream (P. auriga, Valenciennes, 1843) and their application to paternity studies |
title_fullStr |
Development of two new microsatellite multiplex PCRs for three sparid species: Gilthead seabream (Spares auratus L.), red porgy (Pagrus pagrus L.) and redbanded seabream (P. auriga, Valenciennes, 1843) and their application to paternity studies |
title_full_unstemmed |
Development of two new microsatellite multiplex PCRs for three sparid species: Gilthead seabream (Spares auratus L.), red porgy (Pagrus pagrus L.) and redbanded seabream (P. auriga, Valenciennes, 1843) and their application to paternity studies |
title_sort |
development of two new microsatellite multiplex pcrs for three sparid species: gilthead seabream (spares auratus l.), red porgy (pagrus pagrus l.) and redbanded seabream (p. auriga, valenciennes, 1843) and their application to paternity studies |
publisher |
0044-8486 |
publishDate |
2008 |
url |
http://hdl.handle.net/10553/53105 https://doi.org/10.1016/j.aquaculture.2008.07.005 |
long_lat |
ENVELOPE(-45.400,-45.400,-60.633,-60.633) |
geographic |
Rima |
geographic_facet |
Rima |
genre |
Salmo salar |
genre_facet |
Salmo salar |
op_source |
Aquaculture[ISSN 0044-8486],v. 285 (1-4), p. 30-37 |
op_relation |
Aquaculture 285 0044-8486 http://hdl.handle.net/10553/53105 doi:10.1016/j.aquaculture.2008.07.005 000261729200006 1-4 WOS:000261729200006 M-5052-2018 E-3879-2010 Sí |
op_doi |
https://doi.org/10.1016/j.aquaculture.2008.07.005 |
container_title |
Aquaculture |
container_volume |
285 |
container_issue |
1-4 |
container_start_page |
30 |
op_container_end_page |
37 |
_version_ |
1766182695529873408 |