Assessment of Calanus finmarchicus growth and dormancy using the aminoacyl-tRNA synthetases method
We obtained growth rates of the copepod Calanus finmarchicus at different locations across the North Atlantic between May 1998 and June 2004. Animals were incubated for 2–9 days and fed either with natural food assemblages or with cultured algae. During this period, we measured both somatic weight-s...
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Online Access: | http://hdl.handle.net/10553/49888 https://doi.org/10.1093/plankt/fbl049 |
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ftunivlaspalmas:oai:accedacris.ulpgc.es:10553/49888 2023-05-15T15:47:54+02:00 Assessment of Calanus finmarchicus growth and dormancy using the aminoacyl-tRNA synthetases method Yebra, L. Hirst, A. G. Hernández-León, S. 55880102100 7007107826 6701465678 2006 http://hdl.handle.net/10553/49888 https://doi.org/10.1093/plankt/fbl049 eng eng 0142-7873 Journal of Plankton Research 0142-7873 http://hdl.handle.net/10553/49888 doi:10.1093/plankt/fbl049 33947134266 1198 1191 28 Journal of Plankton Research [ISSN 0142-7873], v. 28, p. 1191-1198 Investigación info:eu-repo/semantics/article Article 2006 ftunivlaspalmas https://doi.org/10.1093/plankt/fbl049 2020-02-19T00:08:05Z We obtained growth rates of the copepod Calanus finmarchicus at different locations across the North Atlantic between May 1998 and June 2004. Animals were incubated for 2–9 days and fed either with natural food assemblages or with cultured algae. During this period, we measured both somatic weight-specific growth rates (measured as protein change) and aminoacyl-tRNA synthetases (AARS) activity. We found a highly significant relationship between AARS activity and growth in protein content (R2 = 0.55, P < 0.001). Significant AARS activity also occurred when growth was negative, the relationship predicting an AARS activity level of ≤8.33 nmPPi·mg protein−1·h−1 when somatic growth is zero. This is because AARS activity is expected even when growth is negative, owing to the continued protein turnover in the cells. The AARS method allowed for the first time the study of protein metabolism in overwintering C. finmarchicus. Our study results showed that overwintering copepods had significantly lower values of AARS activity than non-diapausing animals (t = −3.51, P < 0.002). The AARS method opens the possibility to better understand physiology dynamics of deep-water organisms (e.g. the beginning and end of diapause). Article in Journal/Newspaper Calanus finmarchicus North Atlantic Copepods Universidad de Las Palmas de Gran Canaria: Acceda Journal of Plankton Research 28 12 1191 1198 |
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Universidad de Las Palmas de Gran Canaria: Acceda |
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ftunivlaspalmas |
language |
English |
topic |
Investigación |
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Investigación Yebra, L. Hirst, A. G. Hernández-León, S. Assessment of Calanus finmarchicus growth and dormancy using the aminoacyl-tRNA synthetases method |
topic_facet |
Investigación |
description |
We obtained growth rates of the copepod Calanus finmarchicus at different locations across the North Atlantic between May 1998 and June 2004. Animals were incubated for 2–9 days and fed either with natural food assemblages or with cultured algae. During this period, we measured both somatic weight-specific growth rates (measured as protein change) and aminoacyl-tRNA synthetases (AARS) activity. We found a highly significant relationship between AARS activity and growth in protein content (R2 = 0.55, P < 0.001). Significant AARS activity also occurred when growth was negative, the relationship predicting an AARS activity level of ≤8.33 nmPPi·mg protein−1·h−1 when somatic growth is zero. This is because AARS activity is expected even when growth is negative, owing to the continued protein turnover in the cells. The AARS method allowed for the first time the study of protein metabolism in overwintering C. finmarchicus. Our study results showed that overwintering copepods had significantly lower values of AARS activity than non-diapausing animals (t = −3.51, P < 0.002). The AARS method opens the possibility to better understand physiology dynamics of deep-water organisms (e.g. the beginning and end of diapause). |
author2 |
55880102100 7007107826 6701465678 |
format |
Article in Journal/Newspaper |
author |
Yebra, L. Hirst, A. G. Hernández-León, S. |
author_facet |
Yebra, L. Hirst, A. G. Hernández-León, S. |
author_sort |
Yebra, L. |
title |
Assessment of Calanus finmarchicus growth and dormancy using the aminoacyl-tRNA synthetases method |
title_short |
Assessment of Calanus finmarchicus growth and dormancy using the aminoacyl-tRNA synthetases method |
title_full |
Assessment of Calanus finmarchicus growth and dormancy using the aminoacyl-tRNA synthetases method |
title_fullStr |
Assessment of Calanus finmarchicus growth and dormancy using the aminoacyl-tRNA synthetases method |
title_full_unstemmed |
Assessment of Calanus finmarchicus growth and dormancy using the aminoacyl-tRNA synthetases method |
title_sort |
assessment of calanus finmarchicus growth and dormancy using the aminoacyl-trna synthetases method |
publisher |
0142-7873 |
publishDate |
2006 |
url |
http://hdl.handle.net/10553/49888 https://doi.org/10.1093/plankt/fbl049 |
genre |
Calanus finmarchicus North Atlantic Copepods |
genre_facet |
Calanus finmarchicus North Atlantic Copepods |
op_source |
Journal of Plankton Research [ISSN 0142-7873], v. 28, p. 1191-1198 |
op_relation |
Journal of Plankton Research 0142-7873 http://hdl.handle.net/10553/49888 doi:10.1093/plankt/fbl049 33947134266 1198 1191 28 |
op_doi |
https://doi.org/10.1093/plankt/fbl049 |
container_title |
Journal of Plankton Research |
container_volume |
28 |
container_issue |
12 |
container_start_page |
1191 |
op_container_end_page |
1198 |
_version_ |
1766382877603266560 |