Differential innate immune response of European seabass (Dicentrarchus labrax) against Streptococcus iniae
Streptococcus iniae is a Gram-positive bacteria that causes invasive infections with severe septicemia and meningitis, producing high economic losses in marine and continental aquaculture. Head kidney leukocytes of European sea bass (Dicentrarchus labrax) were used to measure the differential innate...
Published in: | Fish & Shellfish Immunology |
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Language: | English |
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Online Access: | http://hdl.handle.net/10553/47222 https://doi.org/10.1016/j.fsi.2015.05.054 |
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ftunivlaspalmas:oai:accedacris.ulpgc.es:10553/47222 2023-05-15T15:32:39+02:00 Differential innate immune response of European seabass (Dicentrarchus labrax) against Streptococcus iniae El Aamri, F. Real Valcárcel, Fernando Acosta, F. Bravo García, Jimena Román, L. Déniz, S. Padilla, D. 55351966600 7005397318 56269311600 23059544100 50661926900 7003859353 7005088705 3017971 32158446 878226 1844262 16153744 28148410 1039753 WOS:El Aamri, F WOS:Real, F WOS:Acosta, F WOS:Bravo, J WOS:Roman, L WOS:Deniz, S WOS:Padilla, D BU-VET 2015 http://hdl.handle.net/10553/47222 https://doi.org/10.1016/j.fsi.2015.05.054 eng eng 1050-4648 Fish and Shellfish Immunology 46 1050-4648 http://hdl.handle.net/10553/47222 doi:10.1016/j.fsi.2015.05.054 84938066597 000363071200031 Sí Fish and Shellfish Immunology[ISSN 1050-4648],v. 46, p. 436-441 310905 Microbiología Salmo-Salar L Atlantic Salmon Rainbow-Trout Measure Degranulation Expression Analysis Respiratory Burst Molecular-Cloning Adaptive Immunity Direct Assay Fish info:eu-repo/semantics/Article Article 2015 ftunivlaspalmas https://doi.org/10.1016/j.fsi.2015.05.054 2021-08-03T23:10:51Z Streptococcus iniae is a Gram-positive bacteria that causes invasive infections with severe septicemia and meningitis, producing high economic losses in marine and continental aquaculture. Head kidney leukocytes of European sea bass (Dicentrarchus labrax) were used to measure the differential innate immune response upon infection with S. iniae. The complete inhibition in the production of intracellular superoxide radicals and total peroxidase content was observed in infected cells. This study also elucidates changes in the relative expression of some immune-related genes. Interleukin 1 beta, tumor necrosis factor-a and interleukin-6 reached a peak of expression at 4-8 h post-infection, subsequently decreasing significantly up to 48 h post-infection. However, interleukin-10 and Mx protein increased over time, reaching the pick of expression at 48 h post-infection, whereas caspase-3 showed down regulation until 48 h post-infection. The in vivo study of immune related genes show the same kinetics of mRNAs expression as in vitro experience. The proinflammatory cytokines mRNA transcription levels peaked at an earlier time in vivo than in vitro system. Our findings indicate that there is a direct relationship between the dissemination of bacteria and the resulting infection-associated inhibition of respiratory burst, apoptosis, and the pro- and anti-inflammatory gene expression profiles. (C) 2015 Published by Elsevier Ltd. 441 436 1,268 3,025 Q1 Q1 Article in Journal/Newspaper Atlantic salmon Salmo salar Universidad de Las Palmas de Gran Canaria: Acceda Fish & Shellfish Immunology 46 2 436 441 |
institution |
Open Polar |
collection |
Universidad de Las Palmas de Gran Canaria: Acceda |
op_collection_id |
ftunivlaspalmas |
language |
English |
topic |
310905 Microbiología Salmo-Salar L Atlantic Salmon Rainbow-Trout Measure Degranulation Expression Analysis Respiratory Burst Molecular-Cloning Adaptive Immunity Direct Assay Fish |
spellingShingle |
310905 Microbiología Salmo-Salar L Atlantic Salmon Rainbow-Trout Measure Degranulation Expression Analysis Respiratory Burst Molecular-Cloning Adaptive Immunity Direct Assay Fish El Aamri, F. Real Valcárcel, Fernando Acosta, F. Bravo García, Jimena Román, L. Déniz, S. Padilla, D. Differential innate immune response of European seabass (Dicentrarchus labrax) against Streptococcus iniae |
topic_facet |
310905 Microbiología Salmo-Salar L Atlantic Salmon Rainbow-Trout Measure Degranulation Expression Analysis Respiratory Burst Molecular-Cloning Adaptive Immunity Direct Assay Fish |
description |
Streptococcus iniae is a Gram-positive bacteria that causes invasive infections with severe septicemia and meningitis, producing high economic losses in marine and continental aquaculture. Head kidney leukocytes of European sea bass (Dicentrarchus labrax) were used to measure the differential innate immune response upon infection with S. iniae. The complete inhibition in the production of intracellular superoxide radicals and total peroxidase content was observed in infected cells. This study also elucidates changes in the relative expression of some immune-related genes. Interleukin 1 beta, tumor necrosis factor-a and interleukin-6 reached a peak of expression at 4-8 h post-infection, subsequently decreasing significantly up to 48 h post-infection. However, interleukin-10 and Mx protein increased over time, reaching the pick of expression at 48 h post-infection, whereas caspase-3 showed down regulation until 48 h post-infection. The in vivo study of immune related genes show the same kinetics of mRNAs expression as in vitro experience. The proinflammatory cytokines mRNA transcription levels peaked at an earlier time in vivo than in vitro system. Our findings indicate that there is a direct relationship between the dissemination of bacteria and the resulting infection-associated inhibition of respiratory burst, apoptosis, and the pro- and anti-inflammatory gene expression profiles. (C) 2015 Published by Elsevier Ltd. 441 436 1,268 3,025 Q1 Q1 |
author2 |
55351966600 7005397318 56269311600 23059544100 50661926900 7003859353 7005088705 3017971 32158446 878226 1844262 16153744 28148410 1039753 WOS:El Aamri, F WOS:Real, F WOS:Acosta, F WOS:Bravo, J WOS:Roman, L WOS:Deniz, S WOS:Padilla, D BU-VET |
format |
Article in Journal/Newspaper |
author |
El Aamri, F. Real Valcárcel, Fernando Acosta, F. Bravo García, Jimena Román, L. Déniz, S. Padilla, D. |
author_facet |
El Aamri, F. Real Valcárcel, Fernando Acosta, F. Bravo García, Jimena Román, L. Déniz, S. Padilla, D. |
author_sort |
El Aamri, F. |
title |
Differential innate immune response of European seabass (Dicentrarchus labrax) against Streptococcus iniae |
title_short |
Differential innate immune response of European seabass (Dicentrarchus labrax) against Streptococcus iniae |
title_full |
Differential innate immune response of European seabass (Dicentrarchus labrax) against Streptococcus iniae |
title_fullStr |
Differential innate immune response of European seabass (Dicentrarchus labrax) against Streptococcus iniae |
title_full_unstemmed |
Differential innate immune response of European seabass (Dicentrarchus labrax) against Streptococcus iniae |
title_sort |
differential innate immune response of european seabass (dicentrarchus labrax) against streptococcus iniae |
publisher |
1050-4648 |
publishDate |
2015 |
url |
http://hdl.handle.net/10553/47222 https://doi.org/10.1016/j.fsi.2015.05.054 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
Fish and Shellfish Immunology[ISSN 1050-4648],v. 46, p. 436-441 |
op_relation |
Fish and Shellfish Immunology 46 1050-4648 http://hdl.handle.net/10553/47222 doi:10.1016/j.fsi.2015.05.054 84938066597 000363071200031 Sí |
op_doi |
https://doi.org/10.1016/j.fsi.2015.05.054 |
container_title |
Fish & Shellfish Immunology |
container_volume |
46 |
container_issue |
2 |
container_start_page |
436 |
op_container_end_page |
441 |
_version_ |
1766363151545139200 |