Influence of different anaesthetic protocols over the sperm quality on the fresh, chilled (4°C) and frozen-thawed epididymal sperm samples in domestic dogs
This study assessed the influence of three different anaesthetic protocols on semen quality obtained from the epididymis. Sixty male dogs undergoing to routine sterilization were assigned to three anaesthetic protocols: thiopental group (TG, n = 20), propofol group (PG, n = 20) and ketamine-dexmedet...
Published in: | Reproduction in Domestic Animals |
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Language: | English |
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2016
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Online Access: | http://hdl.handle.net/10553/42113 https://doi.org/10.1111/rda.12743 |
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ftunivlaspalmas:oai:accedacris.ulpgc.es:10553/42113 2023-05-15T15:51:13+02:00 Influence of different anaesthetic protocols over the sperm quality on the fresh, chilled (4°C) and frozen-thawed epididymal sperm samples in domestic dogs Batista Arteaga, Miguel Vilar Guereño, José Manuel Rosario Medina, Mª Inmaculada Terradas, E. 7005481929 57212844863 7005533720 6603305825 57191107931 30341669 913655 4748603 26949653 WOS:Batista, M WOS:Vilar, J WOS:Rosario, I WOS:Terradas, E 2016 http://hdl.handle.net/10553/42113 https://doi.org/10.1111/rda.12743 eng eng Reproduction in Domestic Animals 51 1439-0531 WoS http://hdl.handle.net/10553/42113 doi:10.1111/rda.12743 84986877718 000388334100016 5 Reproduction In Domestic Animals [ISSN 0936-6768], v. 51 (5), p. 758-765, (Octubre 2016) 310907 Patología 310411 Reproducción Wolves Canis-Lupus Semen Collection Prostatic Fluid Equex Stm Spermatozoa Cryopreservation Motility Storage Time Electroejaculation info:eu-repo/semantics/Article Article 2016 ftunivlaspalmas https://doi.org/10.1111/rda.12743 2020-07-28T23:08:42Z This study assessed the influence of three different anaesthetic protocols on semen quality obtained from the epididymis. Sixty male dogs undergoing to routine sterilization were assigned to three anaesthetic protocols: thiopental group (TG, n = 20), propofol group (PG, n = 20) and ketamine-dexmedetomidine group (KDG, n = 20). Immediately after orchidectomy, the cauda epididymides and vas deferent ducts were isolated and then a retrograde flushing was performed to collect spermatozoa. In experiment 1, after the initial evaluation of the semen (sperm concentration, sperm motility and the percentages of live spermatozoa, abnormal spermatozoa and acrosome membrane integrity), semen samples were diluted in Tris-glucose-egg yolk extender and chilled for 48 hr, and the sperm motility was assessed at 6, 24 and 48 hr. In experiment 2, semen samples were diluted in Tris-glucose-egg yolk extender and chilled for 24 hr, and then samples were frozen in two extenders with different glycerol concentrations, to reach a final concentration of 50-100 × 10(6) spermatozoa ml(-1) , 20% egg yolk, 0.5% Equex and 4% and 5% glycerol, respectively. Mean values of total sperm concentration, sperm viability and the percentages of intact acrosome and abnormal spermatozoa were not significantly different between experimental groups, and therefore, the anaesthetic protocols assessed did not affect sperm parameters mentioned above. However, our study confirmed a detrimental effect of the use of thiopental (TG) over the total sperm motility (p < 0.05) and progressive sperm motility (p < 0.05) of the fresh and chilled epididymal sperm samples. The anaesthetic protocols including the application of propofol or ketamine-dexmedetomidine can be used to recover sperm in domestic canids without significant changes in sperm quality compared when semen is collected routinely and these techniques could be applicable to endangered wild canids. 765 758 8 Article in Journal/Newspaper Canis lupus Universidad de Las Palmas de Gran Canaria: Acceda Reproduction in Domestic Animals 51 5 758 765 |
institution |
Open Polar |
collection |
Universidad de Las Palmas de Gran Canaria: Acceda |
op_collection_id |
ftunivlaspalmas |
language |
English |
topic |
310907 Patología 310411 Reproducción Wolves Canis-Lupus Semen Collection Prostatic Fluid Equex Stm Spermatozoa Cryopreservation Motility Storage Time Electroejaculation |
spellingShingle |
310907 Patología 310411 Reproducción Wolves Canis-Lupus Semen Collection Prostatic Fluid Equex Stm Spermatozoa Cryopreservation Motility Storage Time Electroejaculation Batista Arteaga, Miguel Vilar Guereño, José Manuel Rosario Medina, Mª Inmaculada Terradas, E. Influence of different anaesthetic protocols over the sperm quality on the fresh, chilled (4°C) and frozen-thawed epididymal sperm samples in domestic dogs |
topic_facet |
310907 Patología 310411 Reproducción Wolves Canis-Lupus Semen Collection Prostatic Fluid Equex Stm Spermatozoa Cryopreservation Motility Storage Time Electroejaculation |
description |
This study assessed the influence of three different anaesthetic protocols on semen quality obtained from the epididymis. Sixty male dogs undergoing to routine sterilization were assigned to three anaesthetic protocols: thiopental group (TG, n = 20), propofol group (PG, n = 20) and ketamine-dexmedetomidine group (KDG, n = 20). Immediately after orchidectomy, the cauda epididymides and vas deferent ducts were isolated and then a retrograde flushing was performed to collect spermatozoa. In experiment 1, after the initial evaluation of the semen (sperm concentration, sperm motility and the percentages of live spermatozoa, abnormal spermatozoa and acrosome membrane integrity), semen samples were diluted in Tris-glucose-egg yolk extender and chilled for 48 hr, and the sperm motility was assessed at 6, 24 and 48 hr. In experiment 2, semen samples were diluted in Tris-glucose-egg yolk extender and chilled for 24 hr, and then samples were frozen in two extenders with different glycerol concentrations, to reach a final concentration of 50-100 × 10(6) spermatozoa ml(-1) , 20% egg yolk, 0.5% Equex and 4% and 5% glycerol, respectively. Mean values of total sperm concentration, sperm viability and the percentages of intact acrosome and abnormal spermatozoa were not significantly different between experimental groups, and therefore, the anaesthetic protocols assessed did not affect sperm parameters mentioned above. However, our study confirmed a detrimental effect of the use of thiopental (TG) over the total sperm motility (p < 0.05) and progressive sperm motility (p < 0.05) of the fresh and chilled epididymal sperm samples. The anaesthetic protocols including the application of propofol or ketamine-dexmedetomidine can be used to recover sperm in domestic canids without significant changes in sperm quality compared when semen is collected routinely and these techniques could be applicable to endangered wild canids. 765 758 8 |
author2 |
7005481929 57212844863 7005533720 6603305825 57191107931 30341669 913655 4748603 26949653 WOS:Batista, M WOS:Vilar, J WOS:Rosario, I WOS:Terradas, E |
format |
Article in Journal/Newspaper |
author |
Batista Arteaga, Miguel Vilar Guereño, José Manuel Rosario Medina, Mª Inmaculada Terradas, E. |
author_facet |
Batista Arteaga, Miguel Vilar Guereño, José Manuel Rosario Medina, Mª Inmaculada Terradas, E. |
author_sort |
Batista Arteaga, Miguel |
title |
Influence of different anaesthetic protocols over the sperm quality on the fresh, chilled (4°C) and frozen-thawed epididymal sperm samples in domestic dogs |
title_short |
Influence of different anaesthetic protocols over the sperm quality on the fresh, chilled (4°C) and frozen-thawed epididymal sperm samples in domestic dogs |
title_full |
Influence of different anaesthetic protocols over the sperm quality on the fresh, chilled (4°C) and frozen-thawed epididymal sperm samples in domestic dogs |
title_fullStr |
Influence of different anaesthetic protocols over the sperm quality on the fresh, chilled (4°C) and frozen-thawed epididymal sperm samples in domestic dogs |
title_full_unstemmed |
Influence of different anaesthetic protocols over the sperm quality on the fresh, chilled (4°C) and frozen-thawed epididymal sperm samples in domestic dogs |
title_sort |
influence of different anaesthetic protocols over the sperm quality on the fresh, chilled (4°c) and frozen-thawed epididymal sperm samples in domestic dogs |
publishDate |
2016 |
url |
http://hdl.handle.net/10553/42113 https://doi.org/10.1111/rda.12743 |
genre |
Canis lupus |
genre_facet |
Canis lupus |
op_source |
Reproduction In Domestic Animals [ISSN 0936-6768], v. 51 (5), p. 758-765, (Octubre 2016) |
op_relation |
Reproduction in Domestic Animals 51 1439-0531 WoS http://hdl.handle.net/10553/42113 doi:10.1111/rda.12743 84986877718 000388334100016 5 |
op_doi |
https://doi.org/10.1111/rda.12743 |
container_title |
Reproduction in Domestic Animals |
container_volume |
51 |
container_issue |
5 |
container_start_page |
758 |
op_container_end_page |
765 |
_version_ |
1766386309212930048 |