Phylogenetic reconstruction, histopathological characterization, and virulence determination of a novel fish pathogen, Nocardia brasiliensis
Nocardiosis is a prevalent disease caused by various cosmopolitan aerobic actinomycetes species within the genus Nocardia, which has been documented across diverse aquatic species. Recently, a distinctive disease manifestation resembling classical nocardiosis emerged among cultured meagre (Argyrosom...
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2024
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Online Access: | http://hdl.handle.net/10553/128104 https://doi.org/10.1016/j.aquaculture.2023.740458 |
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ftunivlaspalmas:oai:accedacris.ulpgc.es:10553/128104 2024-02-04T10:02:49+01:00 Phylogenetic reconstruction, histopathological characterization, and virulence determination of a novel fish pathogen, Nocardia brasiliensis Acosta Arbelo, Félix Antonio Vega, Belinda Monzón Atienza, Luis Ernesto Superio, Joshua Torrecillas Burriel,Silvia Gómez Mercader, Antonio Castro Alonso, Pedro Luis Montero Vítores, Daniel Galindo-Villegas, Jorge NO DATA 56269311600 56407037300 57222659745 57208145811 21733894700 58306167500 58760913700 35605929400 6508306690 BU-BAS 2024 http://hdl.handle.net/10553/128104 https://doi.org/10.1016/j.aquaculture.2023.740458 eng eng Exploring The Biological And Sociio-Economic Potential Of New/Emerging Candidate Fish Species For The Expansion Of The European Aquaculture Industry Aquaculture 581 0044-8486 Scopus http://hdl.handle.net/10553/128104 doi:10.1016/j.aquaculture.2023.740458 85179614376 Sí Aquaculture [ISSN 0044-8486], v. 581 310502 Piscicultura 310907 Patología 16 s rRNA sequencing Argyrosomus regius Emerging disease Granuloma MALDI-TOF Nocardiosis Article 2024 ftunivlaspalmas https://doi.org/10.1016/j.aquaculture.2023.740458 2024-01-10T00:15:39Z Nocardiosis is a prevalent disease caused by various cosmopolitan aerobic actinomycetes species within the genus Nocardia, which has been documented across diverse aquatic species. Recently, a distinctive disease manifestation resembling classical nocardiosis emerged among cultured meagre (Argyrosomus regius) along the European coastline adjacent to the North Atlantic Ocean. To identify the causative agent and reproduce the disease, we conducted experiments on specific pathogen-free (SPF) meagre specimens. Employing classical methods, we isolated bacteria from liver granulomas present in multiple diseased specimens. The causative agent was subsequently identified through a combination of differential staining, MALDI-TOF mass spectrometry (MS), genus-specific nested PCR, and sequencing of the 16S ribosomal RNA gene, which facilitated the positive identification of Nocardia brasiliensis. The isolated strain was designated as NORBRACAN1 and considered the causative agent. Remarkably, this marks the first report of this species in fish. The pathogenicity of NORBRACAN1 was further elucidated through in vivo experimentation on a cohort of 360 juvenile meagre. The intraperitoneal administration of NORBRACAN1 led to the development of internal microgranulomas without overt external signs or mortalities. Intriguingly, fish exposed to concentrations below 1 × 106 CFU ml−1 remained unaffected by mortality throughout the two-month trial period. Immunofluorescence assay unveiled the presence of viable bacteria within specific granulomas. Furthermore, our study unveiled the existence of five distinct types of microscopic granulomas characterized by necrotic centers and arranged macrophages. The formation of these granulomas was found to be contingent upon the bacterial dosage. In conclusion, our investigation demonstrates the ability of N. brasiliensis to colonize internal organs in marine fish and induce chronic granulomatosis. This research illuminates the potential pathogenesis of nocardiosis in aquatic species, ... Article in Journal/Newspaper North Atlantic Universidad de Las Palmas de Gran Canaria: Acceda Aquaculture 581 740458 |
institution |
Open Polar |
collection |
Universidad de Las Palmas de Gran Canaria: Acceda |
op_collection_id |
ftunivlaspalmas |
language |
English |
topic |
310502 Piscicultura 310907 Patología 16 s rRNA sequencing Argyrosomus regius Emerging disease Granuloma MALDI-TOF Nocardiosis |
spellingShingle |
310502 Piscicultura 310907 Patología 16 s rRNA sequencing Argyrosomus regius Emerging disease Granuloma MALDI-TOF Nocardiosis Acosta Arbelo, Félix Antonio Vega, Belinda Monzón Atienza, Luis Ernesto Superio, Joshua Torrecillas Burriel,Silvia Gómez Mercader, Antonio Castro Alonso, Pedro Luis Montero Vítores, Daniel Galindo-Villegas, Jorge Phylogenetic reconstruction, histopathological characterization, and virulence determination of a novel fish pathogen, Nocardia brasiliensis |
topic_facet |
310502 Piscicultura 310907 Patología 16 s rRNA sequencing Argyrosomus regius Emerging disease Granuloma MALDI-TOF Nocardiosis |
description |
Nocardiosis is a prevalent disease caused by various cosmopolitan aerobic actinomycetes species within the genus Nocardia, which has been documented across diverse aquatic species. Recently, a distinctive disease manifestation resembling classical nocardiosis emerged among cultured meagre (Argyrosomus regius) along the European coastline adjacent to the North Atlantic Ocean. To identify the causative agent and reproduce the disease, we conducted experiments on specific pathogen-free (SPF) meagre specimens. Employing classical methods, we isolated bacteria from liver granulomas present in multiple diseased specimens. The causative agent was subsequently identified through a combination of differential staining, MALDI-TOF mass spectrometry (MS), genus-specific nested PCR, and sequencing of the 16S ribosomal RNA gene, which facilitated the positive identification of Nocardia brasiliensis. The isolated strain was designated as NORBRACAN1 and considered the causative agent. Remarkably, this marks the first report of this species in fish. The pathogenicity of NORBRACAN1 was further elucidated through in vivo experimentation on a cohort of 360 juvenile meagre. The intraperitoneal administration of NORBRACAN1 led to the development of internal microgranulomas without overt external signs or mortalities. Intriguingly, fish exposed to concentrations below 1 × 106 CFU ml−1 remained unaffected by mortality throughout the two-month trial period. Immunofluorescence assay unveiled the presence of viable bacteria within specific granulomas. Furthermore, our study unveiled the existence of five distinct types of microscopic granulomas characterized by necrotic centers and arranged macrophages. The formation of these granulomas was found to be contingent upon the bacterial dosage. In conclusion, our investigation demonstrates the ability of N. brasiliensis to colonize internal organs in marine fish and induce chronic granulomatosis. This research illuminates the potential pathogenesis of nocardiosis in aquatic species, ... |
author2 |
NO DATA 56269311600 56407037300 57222659745 57208145811 21733894700 58306167500 58760913700 35605929400 6508306690 BU-BAS |
format |
Article in Journal/Newspaper |
author |
Acosta Arbelo, Félix Antonio Vega, Belinda Monzón Atienza, Luis Ernesto Superio, Joshua Torrecillas Burriel,Silvia Gómez Mercader, Antonio Castro Alonso, Pedro Luis Montero Vítores, Daniel Galindo-Villegas, Jorge |
author_facet |
Acosta Arbelo, Félix Antonio Vega, Belinda Monzón Atienza, Luis Ernesto Superio, Joshua Torrecillas Burriel,Silvia Gómez Mercader, Antonio Castro Alonso, Pedro Luis Montero Vítores, Daniel Galindo-Villegas, Jorge |
author_sort |
Acosta Arbelo, Félix Antonio |
title |
Phylogenetic reconstruction, histopathological characterization, and virulence determination of a novel fish pathogen, Nocardia brasiliensis |
title_short |
Phylogenetic reconstruction, histopathological characterization, and virulence determination of a novel fish pathogen, Nocardia brasiliensis |
title_full |
Phylogenetic reconstruction, histopathological characterization, and virulence determination of a novel fish pathogen, Nocardia brasiliensis |
title_fullStr |
Phylogenetic reconstruction, histopathological characterization, and virulence determination of a novel fish pathogen, Nocardia brasiliensis |
title_full_unstemmed |
Phylogenetic reconstruction, histopathological characterization, and virulence determination of a novel fish pathogen, Nocardia brasiliensis |
title_sort |
phylogenetic reconstruction, histopathological characterization, and virulence determination of a novel fish pathogen, nocardia brasiliensis |
publishDate |
2024 |
url |
http://hdl.handle.net/10553/128104 https://doi.org/10.1016/j.aquaculture.2023.740458 |
genre |
North Atlantic |
genre_facet |
North Atlantic |
op_source |
Aquaculture [ISSN 0044-8486], v. 581 |
op_relation |
Exploring The Biological And Sociio-Economic Potential Of New/Emerging Candidate Fish Species For The Expansion Of The European Aquaculture Industry Aquaculture 581 0044-8486 Scopus http://hdl.handle.net/10553/128104 doi:10.1016/j.aquaculture.2023.740458 85179614376 Sí |
op_doi |
https://doi.org/10.1016/j.aquaculture.2023.740458 |
container_title |
Aquaculture |
container_volume |
581 |
container_start_page |
740458 |
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1789969786273267712 |