Effect of Candida antarctica lipase B immobilization on the porous structure of the carrier

• Published in: Macromolecular Bioscience
• Main Authors: Miletic, Nemanja, Vuković, Zorica, Nastasović, Aleksandra, Loos, Katja
• Format: Article in Journal/Newspaper
• Language: English
• Published: Wiley 2011
• Subjects: Candida antarctica Lipase B; Immobilization; Epoxy carrier; Glutaraldehyde modification; Porosity; poly[(glycidyl methacrylate)-co-(ethylene glycol dimethacrylate)]; Antarc*; Antarctica
• Online Access: https://scidar.kg.ac.rs/handle/123456789/19850; https://doi.org/10.1002/mabi.201100127

A series of poly(GMA-co-EGDMA) resins with identical composition but varying particle sizes, pore radii, specific surface areas and specific volumes are studied to assess how Candida antarctica lipase B immobilization affects the porosity of the copolymer particles. Mercury porosimetry reveals a significant change in the average pore size (up to 6.1-fold), the specific surface area (up to 3.2-fold) and the specific volume (up to 2.1-fold) of the epoxy resin. A similar behaviour is observed for glutaraldehyde-modified epoxy resins. The influences of the resin porosity properties on the loading of Candida antarctica lipase B during immobilization and on the hydrolytic activity (hydrolysis of p-nitrophenyl acetate) of the immobilized lipase are studied. Published