Over-Stabilization of Chemically Modified and Cross-Linked Candida antarctica Lipase B Using Various Epoxides and Diepoxides
Candida antarctica lipase B, Cal-B, was (i) chemically modified with various epoxides, or (ii) cross-linked with various diepoxides in order to improve enzyme activity and thermal stability. Changed enzyme structure was confirmed by kinetic resolution of p-nitrophenol acetate with methanol and deter...
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Online Access: | https://scidar.kg.ac.rs/handle/123456789/19835 https://doi.org/10.1071/CH09033 |
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ftunivkragujevac:oai:https://scidar.kg.ac.rs:123456789/19835 2024-09-15T17:46:38+00:00 Over-Stabilization of Chemically Modified and Cross-Linked Candida antarctica Lipase B Using Various Epoxides and Diepoxides Miletic, Nemanja Loos, Katja 2009 application/pdf https://scidar.kg.ac.rs/handle/123456789/19835 https://doi.org/10.1071/CH09033 en eng CSIRO PUBLISHING Miletić, N., Loos, K. (2009): Over-stabilization of chemically modified and cross-linked Candida antarctica lipase B using various epoxides and diepoxides. Australian Journal of Chemistry, 62(8), 799-805. 0004-9425 https://scidar.kg.ac.rs/handle/123456789/19835 doi:10.1071/CH09033 000268955500007 info:eu-repo/semantics/openAccess Australian Journal of Chemistry Candida antarctica Lipase B Epoxides Diepoxides Michaelis–Menten article PublishedVersion 2009 ftunivkragujevac https://doi.org/10.1071/CH09033 2024-07-11T23:30:40Z Candida antarctica lipase B, Cal-B, was (i) chemically modified with various epoxides, or (ii) cross-linked with various diepoxides in order to improve enzyme activity and thermal stability. Changed enzyme structure was confirmed by kinetic resolution of p-nitrophenol acetate with methanol and determination of the kinetic parameters. Thermal stability measurements were carried out for each Cal-B derivative, proving that Cal-B modified with 1,2-epoxypropane and 1,2-epoxypentane, and cross-linked with 1,2,7,8-diepoxyoctane and 1,2,9,10-diepoxydecane showed higher stability than soluble enzyme. The influence of the length of the epoxide arm in the modification process, as well as the length of the spacer arm in the cross-linking process, on the final enzyme preparation properties was investigated. The amount of epoxides and diepoxides used for structural rearrangement played an important role in the derivatives’ characteristics. The influence of the precipitant used and the presence of additives, such as surfactant or crown-ether, during modification and cross-linking processes was also revealed. Published Article in Journal/Newspaper Antarc* Antarctica SCIDAR - A Digital Archive of the University of Kragujevac Australian Journal of Chemistry 62 8 799 |
institution |
Open Polar |
collection |
SCIDAR - A Digital Archive of the University of Kragujevac |
op_collection_id |
ftunivkragujevac |
language |
English |
topic |
Candida antarctica Lipase B Epoxides Diepoxides Michaelis–Menten |
spellingShingle |
Candida antarctica Lipase B Epoxides Diepoxides Michaelis–Menten Miletic, Nemanja Loos, Katja Over-Stabilization of Chemically Modified and Cross-Linked Candida antarctica Lipase B Using Various Epoxides and Diepoxides |
topic_facet |
Candida antarctica Lipase B Epoxides Diepoxides Michaelis–Menten |
description |
Candida antarctica lipase B, Cal-B, was (i) chemically modified with various epoxides, or (ii) cross-linked with various diepoxides in order to improve enzyme activity and thermal stability. Changed enzyme structure was confirmed by kinetic resolution of p-nitrophenol acetate with methanol and determination of the kinetic parameters. Thermal stability measurements were carried out for each Cal-B derivative, proving that Cal-B modified with 1,2-epoxypropane and 1,2-epoxypentane, and cross-linked with 1,2,7,8-diepoxyoctane and 1,2,9,10-diepoxydecane showed higher stability than soluble enzyme. The influence of the length of the epoxide arm in the modification process, as well as the length of the spacer arm in the cross-linking process, on the final enzyme preparation properties was investigated. The amount of epoxides and diepoxides used for structural rearrangement played an important role in the derivatives’ characteristics. The influence of the precipitant used and the presence of additives, such as surfactant or crown-ether, during modification and cross-linking processes was also revealed. Published |
format |
Article in Journal/Newspaper |
author |
Miletic, Nemanja Loos, Katja |
author_facet |
Miletic, Nemanja Loos, Katja |
author_sort |
Miletic, Nemanja |
title |
Over-Stabilization of Chemically Modified and Cross-Linked Candida antarctica Lipase B Using Various Epoxides and Diepoxides |
title_short |
Over-Stabilization of Chemically Modified and Cross-Linked Candida antarctica Lipase B Using Various Epoxides and Diepoxides |
title_full |
Over-Stabilization of Chemically Modified and Cross-Linked Candida antarctica Lipase B Using Various Epoxides and Diepoxides |
title_fullStr |
Over-Stabilization of Chemically Modified and Cross-Linked Candida antarctica Lipase B Using Various Epoxides and Diepoxides |
title_full_unstemmed |
Over-Stabilization of Chemically Modified and Cross-Linked Candida antarctica Lipase B Using Various Epoxides and Diepoxides |
title_sort |
over-stabilization of chemically modified and cross-linked candida antarctica lipase b using various epoxides and diepoxides |
publisher |
CSIRO PUBLISHING |
publishDate |
2009 |
url |
https://scidar.kg.ac.rs/handle/123456789/19835 https://doi.org/10.1071/CH09033 |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_source |
Australian Journal of Chemistry |
op_relation |
Miletić, N., Loos, K. (2009): Over-stabilization of chemically modified and cross-linked Candida antarctica lipase B using various epoxides and diepoxides. Australian Journal of Chemistry, 62(8), 799-805. 0004-9425 https://scidar.kg.ac.rs/handle/123456789/19835 doi:10.1071/CH09033 000268955500007 |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1071/CH09033 |
container_title |
Australian Journal of Chemistry |
container_volume |
62 |
container_issue |
8 |
container_start_page |
799 |
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1810494949430394880 |