Electrospinning of Poly[acrylonitrile‐co‐(glycidyl methacrylate)] Nanofibrous Mats for the Immobilization of Candida Antarctica Lipase B

• Published in: Macromolecular Chemistry and Physics
• Main Authors: Dai, Tianhe, Miletic, Nemanja, Loos, Katja, Elbahri, Mady, Abetz, Volker
• Format: Article in Journal/Newspaper
• Language: English
• Published: Wiley 2010
• Subjects: PANGMA; Candida antarctica lipase B; Immobilization; Novozyme; Electrospinning; Nanofibers; Antarc*; Antarctica
• Online Access: https://scidar.kg.ac.rs/handle/123456789/19825; https://doi.org/10.1002/macp.201000536

PANGMA nanofibers and nanomats with fiber diameters of 200–300 nanometers were fabricated by electrospinning. Cal-B was covalently immobilized onto the PANGMA nanomats via three different immobilization routes. The properties of the Cal-B-immobilized PANGMA nanomats were assayed and compared with the free Cal-B. The observed Cal-B loading on these nanomats is up to 50mg/g, and their hydrolytic activity is up to 2 500 nmol/min/mg, much higher than free enzyme powder and also slightly higher than Novozyme 435. Cal-B immobilized PANGMA nanomats have better reusability, thermal stability, and storage ability than free Cal-B. They retain over 50% of their initial activity after 15 cycles, over 65% after 10 h heat incubation, and over 75% after 30 d storage. Published