Cladonia lichens and their major metabolites as possible natural antioxidant, antimicrobial and anticancer agents
The aim of this study is to investigate invitro antioxidant, antimicrobial, and anticancer activities of the acetone extracts of the lichens Cladonia furcata, Cladonia pyxidata and Cladonia rangiferina and their atranorin and fumarprotocetraric acid constituents. Antioxidant activity was evaluated b...
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ftunivkragujevac:oai:https://scidar.kg.ac.rs:123456789/12376 2023-05-15T15:55:38+02:00 Cladonia lichens and their major metabolites as possible natural antioxidant, antimicrobial and anticancer agents Kosanic, Marijana Ranković B. Stanojkovic, Tatjana Rančič A. Manojlovic, Nedeljko 2014-01-01 application/pdf https://scidar.kg.ac.rs/handle/123456789/12376 https://doi.org/10.1016/j.lwt.2014.04.047 unknown LWT - Food Science and Technology 00236438 https://scidar.kg.ac.rs/handle/123456789/12376 doi:10.1016/j.lwt.2014.04.047 84903784600 restrictedAccess article 2014 ftunivkragujevac https://doi.org/10.1016/j.lwt.2014.04.047 2022-12-13T17:19:49Z The aim of this study is to investigate invitro antioxidant, antimicrobial, and anticancer activities of the acetone extracts of the lichens Cladonia furcata, Cladonia pyxidata and Cladonia rangiferina and their atranorin and fumarprotocetraric acid constituents. Antioxidant activity was evaluated by free radical scavenging, superoxide anion radical scavenging, reducing power, and determination of total phenolic and flavonoid compounds. As a result of the study atranorin had largest free radical scavenging activity with IC50 values 131.48μg/mL. Moreover, the tested samples had effective reducing power and superoxide anion radical scavenging. Total content of phenol and flavonoid in extracts was determined as pyrocatechol equivalent, and as rutin equivalent, respectively. The strong relationships between total phenolic and flavonoid contents and the antioxidant effect of tested extracts were observed. The antimicrobial activity was estimated by determination of the minimal inhibitory concentration by the broth microdilution method. The most active was fumarprotocetraric acid with minimum inhibitory concentration values ranging from 0.031 to 0.125mg/mL. Anticancer activity was tested against FemX (human melanoma) and LS174 (human colon carcinoma) cell lines using MTT method. All samples were found to be strong anticancer activity toward both cell lines with IC50 values ranging from 10.97 to 41.23μg/mL. © 2014 Elsevier Ltd. Article in Journal/Newspaper Cladonia rangiferina SCIDAR - A Digital Archive of the University of Kragujevac LWT - Food Science and Technology 59 1 518 525 |
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Open Polar |
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SCIDAR - A Digital Archive of the University of Kragujevac |
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ftunivkragujevac |
language |
unknown |
description |
The aim of this study is to investigate invitro antioxidant, antimicrobial, and anticancer activities of the acetone extracts of the lichens Cladonia furcata, Cladonia pyxidata and Cladonia rangiferina and their atranorin and fumarprotocetraric acid constituents. Antioxidant activity was evaluated by free radical scavenging, superoxide anion radical scavenging, reducing power, and determination of total phenolic and flavonoid compounds. As a result of the study atranorin had largest free radical scavenging activity with IC50 values 131.48μg/mL. Moreover, the tested samples had effective reducing power and superoxide anion radical scavenging. Total content of phenol and flavonoid in extracts was determined as pyrocatechol equivalent, and as rutin equivalent, respectively. The strong relationships between total phenolic and flavonoid contents and the antioxidant effect of tested extracts were observed. The antimicrobial activity was estimated by determination of the minimal inhibitory concentration by the broth microdilution method. The most active was fumarprotocetraric acid with minimum inhibitory concentration values ranging from 0.031 to 0.125mg/mL. Anticancer activity was tested against FemX (human melanoma) and LS174 (human colon carcinoma) cell lines using MTT method. All samples were found to be strong anticancer activity toward both cell lines with IC50 values ranging from 10.97 to 41.23μg/mL. © 2014 Elsevier Ltd. |
format |
Article in Journal/Newspaper |
author |
Kosanic, Marijana Ranković B. Stanojkovic, Tatjana Rančič A. Manojlovic, Nedeljko |
spellingShingle |
Kosanic, Marijana Ranković B. Stanojkovic, Tatjana Rančič A. Manojlovic, Nedeljko Cladonia lichens and their major metabolites as possible natural antioxidant, antimicrobial and anticancer agents |
author_facet |
Kosanic, Marijana Ranković B. Stanojkovic, Tatjana Rančič A. Manojlovic, Nedeljko |
author_sort |
Kosanic, Marijana |
title |
Cladonia lichens and their major metabolites as possible natural antioxidant, antimicrobial and anticancer agents |
title_short |
Cladonia lichens and their major metabolites as possible natural antioxidant, antimicrobial and anticancer agents |
title_full |
Cladonia lichens and their major metabolites as possible natural antioxidant, antimicrobial and anticancer agents |
title_fullStr |
Cladonia lichens and their major metabolites as possible natural antioxidant, antimicrobial and anticancer agents |
title_full_unstemmed |
Cladonia lichens and their major metabolites as possible natural antioxidant, antimicrobial and anticancer agents |
title_sort |
cladonia lichens and their major metabolites as possible natural antioxidant, antimicrobial and anticancer agents |
publishDate |
2014 |
url |
https://scidar.kg.ac.rs/handle/123456789/12376 https://doi.org/10.1016/j.lwt.2014.04.047 |
genre |
Cladonia rangiferina |
genre_facet |
Cladonia rangiferina |
op_relation |
LWT - Food Science and Technology 00236438 https://scidar.kg.ac.rs/handle/123456789/12376 doi:10.1016/j.lwt.2014.04.047 84903784600 |
op_rights |
restrictedAccess |
op_doi |
https://doi.org/10.1016/j.lwt.2014.04.047 |
container_title |
LWT - Food Science and Technology |
container_volume |
59 |
container_issue |
1 |
container_start_page |
518 |
op_container_end_page |
525 |
_version_ |
1766391131951595520 |