The Diversity, Metabolomics Profiling, and the Pharmacological Potential of Actinomycetes Isolated from the Estremadura Spur Pockmarks (Portugal)
The Estremadura Spur pockmarks are a unique and unexplored ecosystem located in the North Atlantic, off the coast of Portugal. A total of 85 marine-derived actinomycetes were isolated and cultured from sediments collected from this ecosystem at a depth of 200 to 350 m. Nine genera, Streptomyces, Mic...
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Online Access: | https://doi.org/10.3390/md20010021 https://nbn-resolving.org/urn:nbn:de:gbv:8:3-2022-00132-4 https://macau.uni-kiel.de/receive/macau_mods_00002426 https://macau.uni-kiel.de/servlets/MCRFileNodeServlet/macau_derivate_00003546/marinedrugs-20-00021.pdf |
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ftunivkiel:oai:macau.uni-kiel.de:macau_mods_00002426 2024-06-23T07:55:13+00:00 The Diversity, Metabolomics Profiling, and the Pharmacological Potential of Actinomycetes Isolated from the Estremadura Spur Pockmarks (Portugal) Pinto-Almeida, António Bauermeister, Anelize Luppino, Luca Grilo, Inês R Oliveira, Juliana Sousa, Joana R Petras, Daniel Rodrigues, Clara F Prieto-Davó, Alejandra Tasdemir, Deniz Sobral, Rita G Gaudêncio, Susana P 2021 https://doi.org/10.3390/md20010021 https://nbn-resolving.org/urn:nbn:de:gbv:8:3-2022-00132-4 https://macau.uni-kiel.de/receive/macau_mods_00002426 https://macau.uni-kiel.de/servlets/MCRFileNodeServlet/macau_derivate_00003546/marinedrugs-20-00021.pdf eng eng Marine drugs -- 1660-3397 https://doi.org/10.3390/md20010021 https://nbn-resolving.org/urn:nbn:de:gbv:8:3-2022-00132-4 https://macau.uni-kiel.de/receive/macau_mods_00002426 https://macau.uni-kiel.de/servlets/MCRFileNodeServlet/macau_derivate_00003546/marinedrugs-20-00021.pdf https://creativecommons.org/licenses/by/4.0/ info:eu-repo/semantics/openAccess article ScholarlyArticle ddc:333.7 ddc:570 Published Version Cell Line Tumor Animals Humans Actinobacteria Antineoplastic Agents Biological Products Antioxidants Anti-Bacterial Agents Ecosystem Phylogeny Portugal Metabolomics Aquatic Organisms HaCaT Cells Antimicrobial activity marine natural products Marine-derived actinomycetes Blue Biotechnology Molecular Networking Estremadura Spur Pockmarks Qemistree article Text doc-type:Article 2021 ftunivkiel https://doi.org/10.3390/md20010021 2024-06-12T14:18:24Z The Estremadura Spur pockmarks are a unique and unexplored ecosystem located in the North Atlantic, off the coast of Portugal. A total of 85 marine-derived actinomycetes were isolated and cultured from sediments collected from this ecosystem at a depth of 200 to 350 m. Nine genera, Streptomyces, Micromonospora, Saccharopolyspora, Actinomadura, Actinopolymorpha, Nocardiopsis, Saccharomonospora, Stackebrandtia, and Verrucosispora were identified by 16S rRNA gene sequencing analyses, from which the first two were the most predominant. Non-targeted LC-MS/MS, in combination with molecular networking, revealed high metabolite diversity, including several known metabolites, such as surugamide, antimycin, etamycin, physostigmine, desferrioxamine, ikarugamycin, piericidine, and rakicidin derivatives, as well as numerous unidentified metabolites. Taxonomy was the strongest parameter influencing the metabolite production, highlighting the different biosynthetic potentials of phylogenetically related actinomycetes; the majority of the chemical classes can be used as chemotaxonomic markers, as the metabolite distribution was mostly genera-specific. The EtOAc extracts of the actinomycete isolates demonstrated antimicrobial and antioxidant activity. Altogether, this study demonstrates that the Estremadura Spur is a source of actinomycetes with potential applications for biotechnology. It highlights the importance of investigating actinomycetes from unique ecosystems, such as pockmarks, as the metabolite production reflects their adaptation to this habitat. Article in Journal/Newspaper North Atlantic MACAU: Open Access Repository of Kiel University Marine Drugs 20 1 21 |
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MACAU: Open Access Repository of Kiel University |
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ftunivkiel |
language |
English |
topic |
article ScholarlyArticle ddc:333.7 ddc:570 Published Version Cell Line Tumor Animals Humans Actinobacteria Antineoplastic Agents Biological Products Antioxidants Anti-Bacterial Agents Ecosystem Phylogeny Portugal Metabolomics Aquatic Organisms HaCaT Cells Antimicrobial activity marine natural products Marine-derived actinomycetes Blue Biotechnology Molecular Networking Estremadura Spur Pockmarks Qemistree |
spellingShingle |
article ScholarlyArticle ddc:333.7 ddc:570 Published Version Cell Line Tumor Animals Humans Actinobacteria Antineoplastic Agents Biological Products Antioxidants Anti-Bacterial Agents Ecosystem Phylogeny Portugal Metabolomics Aquatic Organisms HaCaT Cells Antimicrobial activity marine natural products Marine-derived actinomycetes Blue Biotechnology Molecular Networking Estremadura Spur Pockmarks Qemistree Pinto-Almeida, António Bauermeister, Anelize Luppino, Luca Grilo, Inês R Oliveira, Juliana Sousa, Joana R Petras, Daniel Rodrigues, Clara F Prieto-Davó, Alejandra Tasdemir, Deniz Sobral, Rita G Gaudêncio, Susana P The Diversity, Metabolomics Profiling, and the Pharmacological Potential of Actinomycetes Isolated from the Estremadura Spur Pockmarks (Portugal) |
topic_facet |
article ScholarlyArticle ddc:333.7 ddc:570 Published Version Cell Line Tumor Animals Humans Actinobacteria Antineoplastic Agents Biological Products Antioxidants Anti-Bacterial Agents Ecosystem Phylogeny Portugal Metabolomics Aquatic Organisms HaCaT Cells Antimicrobial activity marine natural products Marine-derived actinomycetes Blue Biotechnology Molecular Networking Estremadura Spur Pockmarks Qemistree |
description |
The Estremadura Spur pockmarks are a unique and unexplored ecosystem located in the North Atlantic, off the coast of Portugal. A total of 85 marine-derived actinomycetes were isolated and cultured from sediments collected from this ecosystem at a depth of 200 to 350 m. Nine genera, Streptomyces, Micromonospora, Saccharopolyspora, Actinomadura, Actinopolymorpha, Nocardiopsis, Saccharomonospora, Stackebrandtia, and Verrucosispora were identified by 16S rRNA gene sequencing analyses, from which the first two were the most predominant. Non-targeted LC-MS/MS, in combination with molecular networking, revealed high metabolite diversity, including several known metabolites, such as surugamide, antimycin, etamycin, physostigmine, desferrioxamine, ikarugamycin, piericidine, and rakicidin derivatives, as well as numerous unidentified metabolites. Taxonomy was the strongest parameter influencing the metabolite production, highlighting the different biosynthetic potentials of phylogenetically related actinomycetes; the majority of the chemical classes can be used as chemotaxonomic markers, as the metabolite distribution was mostly genera-specific. The EtOAc extracts of the actinomycete isolates demonstrated antimicrobial and antioxidant activity. Altogether, this study demonstrates that the Estremadura Spur is a source of actinomycetes with potential applications for biotechnology. It highlights the importance of investigating actinomycetes from unique ecosystems, such as pockmarks, as the metabolite production reflects their adaptation to this habitat. |
format |
Article in Journal/Newspaper |
author |
Pinto-Almeida, António Bauermeister, Anelize Luppino, Luca Grilo, Inês R Oliveira, Juliana Sousa, Joana R Petras, Daniel Rodrigues, Clara F Prieto-Davó, Alejandra Tasdemir, Deniz Sobral, Rita G Gaudêncio, Susana P |
author_facet |
Pinto-Almeida, António Bauermeister, Anelize Luppino, Luca Grilo, Inês R Oliveira, Juliana Sousa, Joana R Petras, Daniel Rodrigues, Clara F Prieto-Davó, Alejandra Tasdemir, Deniz Sobral, Rita G Gaudêncio, Susana P |
author_sort |
Pinto-Almeida, António |
title |
The Diversity, Metabolomics Profiling, and the Pharmacological Potential of Actinomycetes Isolated from the Estremadura Spur Pockmarks (Portugal) |
title_short |
The Diversity, Metabolomics Profiling, and the Pharmacological Potential of Actinomycetes Isolated from the Estremadura Spur Pockmarks (Portugal) |
title_full |
The Diversity, Metabolomics Profiling, and the Pharmacological Potential of Actinomycetes Isolated from the Estremadura Spur Pockmarks (Portugal) |
title_fullStr |
The Diversity, Metabolomics Profiling, and the Pharmacological Potential of Actinomycetes Isolated from the Estremadura Spur Pockmarks (Portugal) |
title_full_unstemmed |
The Diversity, Metabolomics Profiling, and the Pharmacological Potential of Actinomycetes Isolated from the Estremadura Spur Pockmarks (Portugal) |
title_sort |
diversity, metabolomics profiling, and the pharmacological potential of actinomycetes isolated from the estremadura spur pockmarks (portugal) |
publishDate |
2021 |
url |
https://doi.org/10.3390/md20010021 https://nbn-resolving.org/urn:nbn:de:gbv:8:3-2022-00132-4 https://macau.uni-kiel.de/receive/macau_mods_00002426 https://macau.uni-kiel.de/servlets/MCRFileNodeServlet/macau_derivate_00003546/marinedrugs-20-00021.pdf |
genre |
North Atlantic |
genre_facet |
North Atlantic |
op_relation |
Marine drugs -- 1660-3397 https://doi.org/10.3390/md20010021 https://nbn-resolving.org/urn:nbn:de:gbv:8:3-2022-00132-4 https://macau.uni-kiel.de/receive/macau_mods_00002426 https://macau.uni-kiel.de/servlets/MCRFileNodeServlet/macau_derivate_00003546/marinedrugs-20-00021.pdf |
op_rights |
https://creativecommons.org/licenses/by/4.0/ info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.3390/md20010021 |
container_title |
Marine Drugs |
container_volume |
20 |
container_issue |
1 |
container_start_page |
21 |
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1802647713261551616 |