Linear Aminolipids with Moderate Antimicrobial Activity from the Antarctic Gram-Negative Bacterium Aequorivita sp.

The combination of LC-MS/MS based metabolomics approach and anti-MRSA activity-guided fractionation scheme was applied on the Gram-negative bacterium Aequorivita sp. isolated from shallow Antarctic sea sediment using a miniaturized culture chip technique. This methodology afforded the isolation of t...

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Bibliographic Details
Published in:Marine Drugs
Main Authors: Chianese, Giuseppina, Esposito, Fortunato Palma, Parrot, Delphine, Ingham, Colin, de Pascale, Donatella, Tasdemir, Deniz
Format: Article in Journal/Newspaper
Language:English
Published: 2018
Subjects:
article
ScholarlyArticle
ddc:333.7
Published version
Flavobacteriaceae
Fatty Acids
Amino Acids
Anti-Bacterial Agents
Chemical Fractionation
Fourier Transform Infrared Spectroscopy
Microbial Sensitivity Tests
DNA Sequence Analysis
Seawater
Molecular Structure
Geologic Sediments
Antarctic Regions
Metabolomics
Methicillin-Resistant Staphylococcus aureus
MRSA
LC-MS/MS
Gram-negative Bacterium
Aequorivita
Linear Aminolipid
Miniaturized Culture Chip
Antarctic
The Antarctic
Antarc*
Online Access:https://doi.org/10.3390/md16060187
https://nbn-resolving.org/urn:nbn:de:gbv:8-mods-2019-00146-1
https://macau.uni-kiel.de/receive/macau_mods_00000106
https://macau.uni-kiel.de/servlets/MCRFileNodeServlet/macau_derivate_00001055/marinedrugs-16-00187-v3.pdf
Description
Summary:The combination of LC-MS/MS based metabolomics approach and anti-MRSA activity-guided fractionation scheme was applied on the Gram-negative bacterium Aequorivita sp. isolated from shallow Antarctic sea sediment using a miniaturized culture chip technique. This methodology afforded the isolation of three new (1⁻3) and four known (4⁻7) N-terminal glycine- or serine-bearing iso-fatty acid amides esterified with another iso-fatty acid through their C-3 hydroxy groups. The chemical structures of the new compounds were elucidated using a set of spectroscopic (NMR, [α]D and FT-IR) and spectrometric (HRMS, HRMS/MS) methods. The aminolipids possessing an N-terminal glycine unit (1, 2, 4, 5) showed moderate in vitro antimicrobial activity against MRSA (IC50 values 22⁻145 μg/mL). This is the first in-depth chemistry and biological activity study performed on the microbial genus Aequorivita.

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