Chronic stress in fish: Investigation of the cellular response to persistent environmental hypercapnia and malnutrition in two marine fish species, turbot (Psetta maxima) and cod (Gadus morhua) via RT-qPCR
For finfish, coping with stress is not only relevant in the context of ecology but has also garnered lots of attention with respect to animal welfare in aquaculture. Yet, In particular the response to chronic stress still raises many questions regarding regulatory dynamics and mechanisms. I investig...
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ftunivkiel:oai:macau.uni-kiel.de:diss_mods_00024351 2024-06-23T07:51:07+00:00 Chronic stress in fish: Investigation of the cellular response to persistent environmental hypercapnia and malnutrition in two marine fish species, turbot (Psetta maxima) and cod (Gadus morhua) via RT-qPCR Chronischer Stress bei Fischen: Untersuchung der zellulären Antwort auf anhaltende Hyperkapnie und Fehlernährung in zwei marinen Fischarten, Steinbutt (Psetta maxima) und Kabeljau (Gadus morhua), mittels RT-qPCR Hermann, Bernd Timo Hanel, Reinhold Reusch, Thorsten 2018 https://nbn-resolving.org/urn:nbn:de:gbv:8-diss-243511 https://macau.uni-kiel.de/receive/diss_mods_00024351 https://macau.uni-kiel.de/servlets/MCRFileNodeServlet/dissertation_derivate_00008017/BTHermann_Dissertation.pdf eng eng https://nbn-resolving.org/urn:nbn:de:gbv:8-diss-243511 https://macau.uni-kiel.de/receive/diss_mods_00024351 https://macau.uni-kiel.de/servlets/MCRFileNodeServlet/dissertation_derivate_00008017/BTHermann_Dissertation.pdf https://rightsstatements.org/page/InC/1.0/ info:eu-repo/semantics/openAccess thesis ddc:570 stress gene expression turbot cod hypercapnia malnutrition animal welfare aquaculture ocean acidification Genexpression Steinbutt Dorsch Hyperkapnie Fehlernährung Tierschutz Aquakultur Ozeanversauerung dissertation Text doc-type:PhDThesis 2018 ftunivkiel 2024-06-12T14:19:39Z For finfish, coping with stress is not only relevant in the context of ecology but has also garnered lots of attention with respect to animal welfare in aquaculture. Yet, In particular the response to chronic stress still raises many questions regarding regulatory dynamics and mechanisms. I investigated the impact of two chronic stressors, relevant for aquaculture and ecology (hypercapnia and malnutrition), in two fish species, turbot (Psetta maxima) and Atlantic cod (Gadus morhua) via gene expression analysis. The aim of this study was to gain new insights in the respective coping mechanisms and detect potential communalities. Further, results were also screened for putative biomarkers for the detection and monitoring of environmental and culture induced stress. In each experiment, data pointed to divergent changes in gene expression between treatment levels, indicating different coping strategies dependent on the respective stress intensity. Further, my findings implied overall changes in lipid- and fatty acid metabolism as general part of the cellular response to chronic stress. Hints for additional hypoxia-like effects, reduced metabolic activity and increasing oxidative stress varied between the experiments, but seemed to increase parallel to the severity of the stressor. Several cues suggested that especially reactive oxygen species may play a central role in mediating both, regulation of gene expression and detrimental consequences of chronic stress. These findings provide new insights in the cellular response to chronic stress in fish and could help to improve contemporary stress and welfare concepts. The regulatory and damaging effects of reactive oxygen species provide a mechanistic explanation for divergent gene expression patterns and may also explain wear and tear like effects. Finally, I was able to identify multiple genes, which can be utilized as stress biomarkers in future studies. Für Fische ist die Bewältigung von Stress nicht nur im ökologischen Kontext, sondern auch in Hinblick auf ... Doctoral or Postdoctoral Thesis atlantic cod Gadus morhua kabeljau Ocean acidification Turbot Dorsch MACAU: Open Access Repository of Kiel University |
institution |
Open Polar |
collection |
MACAU: Open Access Repository of Kiel University |
op_collection_id |
ftunivkiel |
language |
English |
topic |
thesis ddc:570 stress gene expression turbot cod hypercapnia malnutrition animal welfare aquaculture ocean acidification Genexpression Steinbutt Dorsch Hyperkapnie Fehlernährung Tierschutz Aquakultur Ozeanversauerung |
spellingShingle |
thesis ddc:570 stress gene expression turbot cod hypercapnia malnutrition animal welfare aquaculture ocean acidification Genexpression Steinbutt Dorsch Hyperkapnie Fehlernährung Tierschutz Aquakultur Ozeanversauerung Hermann, Bernd Timo Chronic stress in fish: Investigation of the cellular response to persistent environmental hypercapnia and malnutrition in two marine fish species, turbot (Psetta maxima) and cod (Gadus morhua) via RT-qPCR |
topic_facet |
thesis ddc:570 stress gene expression turbot cod hypercapnia malnutrition animal welfare aquaculture ocean acidification Genexpression Steinbutt Dorsch Hyperkapnie Fehlernährung Tierschutz Aquakultur Ozeanversauerung |
description |
For finfish, coping with stress is not only relevant in the context of ecology but has also garnered lots of attention with respect to animal welfare in aquaculture. Yet, In particular the response to chronic stress still raises many questions regarding regulatory dynamics and mechanisms. I investigated the impact of two chronic stressors, relevant for aquaculture and ecology (hypercapnia and malnutrition), in two fish species, turbot (Psetta maxima) and Atlantic cod (Gadus morhua) via gene expression analysis. The aim of this study was to gain new insights in the respective coping mechanisms and detect potential communalities. Further, results were also screened for putative biomarkers for the detection and monitoring of environmental and culture induced stress. In each experiment, data pointed to divergent changes in gene expression between treatment levels, indicating different coping strategies dependent on the respective stress intensity. Further, my findings implied overall changes in lipid- and fatty acid metabolism as general part of the cellular response to chronic stress. Hints for additional hypoxia-like effects, reduced metabolic activity and increasing oxidative stress varied between the experiments, but seemed to increase parallel to the severity of the stressor. Several cues suggested that especially reactive oxygen species may play a central role in mediating both, regulation of gene expression and detrimental consequences of chronic stress. These findings provide new insights in the cellular response to chronic stress in fish and could help to improve contemporary stress and welfare concepts. The regulatory and damaging effects of reactive oxygen species provide a mechanistic explanation for divergent gene expression patterns and may also explain wear and tear like effects. Finally, I was able to identify multiple genes, which can be utilized as stress biomarkers in future studies. Für Fische ist die Bewältigung von Stress nicht nur im ökologischen Kontext, sondern auch in Hinblick auf ... |
author2 |
Hanel, Reinhold Reusch, Thorsten |
format |
Doctoral or Postdoctoral Thesis |
author |
Hermann, Bernd Timo |
author_facet |
Hermann, Bernd Timo |
author_sort |
Hermann, Bernd Timo |
title |
Chronic stress in fish: Investigation of the cellular response to persistent environmental hypercapnia and malnutrition in two marine fish species, turbot (Psetta maxima) and cod (Gadus morhua) via RT-qPCR |
title_short |
Chronic stress in fish: Investigation of the cellular response to persistent environmental hypercapnia and malnutrition in two marine fish species, turbot (Psetta maxima) and cod (Gadus morhua) via RT-qPCR |
title_full |
Chronic stress in fish: Investigation of the cellular response to persistent environmental hypercapnia and malnutrition in two marine fish species, turbot (Psetta maxima) and cod (Gadus morhua) via RT-qPCR |
title_fullStr |
Chronic stress in fish: Investigation of the cellular response to persistent environmental hypercapnia and malnutrition in two marine fish species, turbot (Psetta maxima) and cod (Gadus morhua) via RT-qPCR |
title_full_unstemmed |
Chronic stress in fish: Investigation of the cellular response to persistent environmental hypercapnia and malnutrition in two marine fish species, turbot (Psetta maxima) and cod (Gadus morhua) via RT-qPCR |
title_sort |
chronic stress in fish: investigation of the cellular response to persistent environmental hypercapnia and malnutrition in two marine fish species, turbot (psetta maxima) and cod (gadus morhua) via rt-qpcr |
publishDate |
2018 |
url |
https://nbn-resolving.org/urn:nbn:de:gbv:8-diss-243511 https://macau.uni-kiel.de/receive/diss_mods_00024351 https://macau.uni-kiel.de/servlets/MCRFileNodeServlet/dissertation_derivate_00008017/BTHermann_Dissertation.pdf |
genre |
atlantic cod Gadus morhua kabeljau Ocean acidification Turbot Dorsch |
genre_facet |
atlantic cod Gadus morhua kabeljau Ocean acidification Turbot Dorsch |
op_relation |
https://nbn-resolving.org/urn:nbn:de:gbv:8-diss-243511 https://macau.uni-kiel.de/receive/diss_mods_00024351 https://macau.uni-kiel.de/servlets/MCRFileNodeServlet/dissertation_derivate_00008017/BTHermann_Dissertation.pdf |
op_rights |
https://rightsstatements.org/page/InC/1.0/ info:eu-repo/semantics/openAccess |
_version_ |
1802642111844057088 |