Assessing phylogenetic delineations of taxa of filamentous Xanthophyceae (Stramenopiles) using DNA sequence analyses and morphology
This thesis focuses on assessing taxonomic boundaries among the filamentous members of “yellow-green algae”, i.e. the class Xanthophyceae (Stramenopiles), which are widespread microalgae in freshwater or terrestrial habitats. The scarcity of morphological characters as well as their considerable pla...
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ftunivkiel:oai:macau.uni-kiel.de:diss_mods_00018521 2024-06-23T07:45:49+00:00 Assessing phylogenetic delineations of taxa of filamentous Xanthophyceae (Stramenopiles) using DNA sequence analyses and morphology Einschätzen phylogenetischer Abgrenzungen von Taxa fademförmiger Xanthophyceae (Stramenopiles) mithilfe von DNA Sequenzanalysen und Morphologie Rybalka, Nataliya Schulz, Rüdiger Ober, Dietrich 2015 https://nbn-resolving.org/urn:nbn:de:gbv:8-diss-185215 https://macau.uni-kiel.de/receive/diss_mods_00018521 https://macau.uni-kiel.de/servlets/MCRFileNodeServlet/dissertation_derivate_00006515/Rybalka_PhD_thesis_final_150812publ.pdf eng eng https://nbn-resolving.org/urn:nbn:de:gbv:8-diss-185215 https://macau.uni-kiel.de/receive/diss_mods_00018521 https://macau.uni-kiel.de/servlets/MCRFileNodeServlet/dissertation_derivate_00006515/Rybalka_PhD_thesis_final_150812publ.pdf https://rightsstatements.org/page/InC/1.0/ info:eu-repo/semantics/openAccess thesis ddc:580 Xanthophyceae Stramenopiles algae Taxonomy phylogenetic analyses of DNA Algen Taxonomie phylogenetische Analysen von DNA-Sequenzen dissertation Text doc-type:PhDThesis 2015 ftunivkiel 2024-06-12T14:20:04Z This thesis focuses on assessing taxonomic boundaries among the filamentous members of “yellow-green algae”, i.e. the class Xanthophyceae (Stramenopiles), which are widespread microalgae in freshwater or terrestrial habitats. The scarcity of morphological characters as well as their considerable plasticity requires the employment of molecular marker sequences to define monophyletic genera and species as well as for the unambiguous identification of new isolates. The genetic diversity and species boundaries within genera of Tribonemataceae, i.e Xanthonema Silva, Bumilleria Borzì, Bumilleriopsis Printz, Pseudobumilleriopsis Deason & Bold and Tribonema Derbès & Solier, were assessed using the highly variable psbA/rbcL spacer region as well as the more conserved full rbcL gene sequences. A method and a reference data base for the unambiguous identification of species of Heterococcus Chodat which uses DNA sequence variation in markers from plastid and nuclear genomes were established. In all studied cases the molecular-based species boundaries were more conservative than those defined by morphology. Within a single species almost identical genotypes were repeatedly recovered from strains of different geographic origins. However, the genotypes recovered from Antarctic strains were distinct from those of temperate regions. Die vorliegende Arbeit hat zum Ziel taxonomische Abgrenzungen bei fadenförmigen „Gelbgrünalgen“, d.h. Vertretern der Klasse Xanthophyceae (Stramenopiles), festzustellen. Fadenförmige Xanthophyceae sind weitverbreitete Mikroalgen im Süßwasser und in terrestrischen Lebensräumen. Da diese Algen arm an morphologischen Merkmalen sind und diese zudem eine beträchtliche Plastizität aufweisen, ist es notwendig, molekulare Markersequenzen einzusetzen, um monophyletische Gattungen und Arten zu definieren und neue Isolate auf Artniveau eindeutig bestimmen zu können. Die genetische Diversität und die Abgrenzungen von Gattungen der Tribonemataceae, d.h. Xanthonema Silva, Bumilleria Borzì, Bumilleriopsis ... Doctoral or Postdoctoral Thesis Antarc* Antarctic MACAU: Open Access Repository of Kiel University Antarctic |
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Open Polar |
collection |
MACAU: Open Access Repository of Kiel University |
op_collection_id |
ftunivkiel |
language |
English |
topic |
thesis ddc:580 Xanthophyceae Stramenopiles algae Taxonomy phylogenetic analyses of DNA Algen Taxonomie phylogenetische Analysen von DNA-Sequenzen |
spellingShingle |
thesis ddc:580 Xanthophyceae Stramenopiles algae Taxonomy phylogenetic analyses of DNA Algen Taxonomie phylogenetische Analysen von DNA-Sequenzen Rybalka, Nataliya Assessing phylogenetic delineations of taxa of filamentous Xanthophyceae (Stramenopiles) using DNA sequence analyses and morphology |
topic_facet |
thesis ddc:580 Xanthophyceae Stramenopiles algae Taxonomy phylogenetic analyses of DNA Algen Taxonomie phylogenetische Analysen von DNA-Sequenzen |
description |
This thesis focuses on assessing taxonomic boundaries among the filamentous members of “yellow-green algae”, i.e. the class Xanthophyceae (Stramenopiles), which are widespread microalgae in freshwater or terrestrial habitats. The scarcity of morphological characters as well as their considerable plasticity requires the employment of molecular marker sequences to define monophyletic genera and species as well as for the unambiguous identification of new isolates. The genetic diversity and species boundaries within genera of Tribonemataceae, i.e Xanthonema Silva, Bumilleria Borzì, Bumilleriopsis Printz, Pseudobumilleriopsis Deason & Bold and Tribonema Derbès & Solier, were assessed using the highly variable psbA/rbcL spacer region as well as the more conserved full rbcL gene sequences. A method and a reference data base for the unambiguous identification of species of Heterococcus Chodat which uses DNA sequence variation in markers from plastid and nuclear genomes were established. In all studied cases the molecular-based species boundaries were more conservative than those defined by morphology. Within a single species almost identical genotypes were repeatedly recovered from strains of different geographic origins. However, the genotypes recovered from Antarctic strains were distinct from those of temperate regions. Die vorliegende Arbeit hat zum Ziel taxonomische Abgrenzungen bei fadenförmigen „Gelbgrünalgen“, d.h. Vertretern der Klasse Xanthophyceae (Stramenopiles), festzustellen. Fadenförmige Xanthophyceae sind weitverbreitete Mikroalgen im Süßwasser und in terrestrischen Lebensräumen. Da diese Algen arm an morphologischen Merkmalen sind und diese zudem eine beträchtliche Plastizität aufweisen, ist es notwendig, molekulare Markersequenzen einzusetzen, um monophyletische Gattungen und Arten zu definieren und neue Isolate auf Artniveau eindeutig bestimmen zu können. Die genetische Diversität und die Abgrenzungen von Gattungen der Tribonemataceae, d.h. Xanthonema Silva, Bumilleria Borzì, Bumilleriopsis ... |
author2 |
Schulz, Rüdiger Ober, Dietrich |
format |
Doctoral or Postdoctoral Thesis |
author |
Rybalka, Nataliya |
author_facet |
Rybalka, Nataliya |
author_sort |
Rybalka, Nataliya |
title |
Assessing phylogenetic delineations of taxa of filamentous Xanthophyceae (Stramenopiles) using DNA sequence analyses and morphology |
title_short |
Assessing phylogenetic delineations of taxa of filamentous Xanthophyceae (Stramenopiles) using DNA sequence analyses and morphology |
title_full |
Assessing phylogenetic delineations of taxa of filamentous Xanthophyceae (Stramenopiles) using DNA sequence analyses and morphology |
title_fullStr |
Assessing phylogenetic delineations of taxa of filamentous Xanthophyceae (Stramenopiles) using DNA sequence analyses and morphology |
title_full_unstemmed |
Assessing phylogenetic delineations of taxa of filamentous Xanthophyceae (Stramenopiles) using DNA sequence analyses and morphology |
title_sort |
assessing phylogenetic delineations of taxa of filamentous xanthophyceae (stramenopiles) using dna sequence analyses and morphology |
publishDate |
2015 |
url |
https://nbn-resolving.org/urn:nbn:de:gbv:8-diss-185215 https://macau.uni-kiel.de/receive/diss_mods_00018521 https://macau.uni-kiel.de/servlets/MCRFileNodeServlet/dissertation_derivate_00006515/Rybalka_PhD_thesis_final_150812publ.pdf |
geographic |
Antarctic |
geographic_facet |
Antarctic |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_relation |
https://nbn-resolving.org/urn:nbn:de:gbv:8-diss-185215 https://macau.uni-kiel.de/receive/diss_mods_00018521 https://macau.uni-kiel.de/servlets/MCRFileNodeServlet/dissertation_derivate_00006515/Rybalka_PhD_thesis_final_150812publ.pdf |
op_rights |
https://rightsstatements.org/page/InC/1.0/ info:eu-repo/semantics/openAccess |
_version_ |
1802642492065054720 |