Understanding drivers of antibiotic resistance genes in High Arctic soil ecosystems
This work is licensed under a Creative Commons Attribution 4.0 International License. Soils in tropical and temperate locations are known to be a sink for the genetic potential of anthropogenic-driven acquired antibiotic resistance (AR). In contrast, accumulation of acquired AR is less probable in m...
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ftunivkansas:oai:kuscholarworks.ku.edu:1808/31064 2023-05-15T14:26:14+02:00 Understanding drivers of antibiotic resistance genes in High Arctic soil ecosystems McCann, Clare M. Christgen, Beate Roberts, Jennifer A. Su, Jian-Qiang Arnold, Kathryn E. Gray, Neil D. Zhu, Yong-Guan Graham, David W. 2021-01-07T21:55:55Z http://hdl.handle.net/1808/31064 https://doi.org/10.1016/j.envint.2019.01.034 unknown Elsevier Clare M. McCann, Beate Christgen, Jennifer A. Roberts, Jian-Qiang Su, Kathryn E. Arnold, Neil D. Gray, Yong-Guan Zhu, David W. Graham, "Understanding drivers of antibiotic resistance genes in High Arctic soil ecosystems", Environment International, Volume 125, 2019, Pages 497-504, ISSN 0160-4120, https://doi.org/10.1016/j.envint.2019.01.034. http://hdl.handle.net/1808/31064 doi:10.1016/j.envint.2019.01.034 © 2019 The Authors. Published by Elsevier Ltd. http://creativecommons.org/licenses/by/4.0/ openAccess CC-BY High Arctic Antibiotic resistance International spread Wildlife Geochemistry High throughput qPCR Article 2021 ftunivkansas https://doi.org/10.1016/j.envint.2019.01.034 2022-08-26T13:25:58Z This work is licensed under a Creative Commons Attribution 4.0 International License. Soils in tropical and temperate locations are known to be a sink for the genetic potential of anthropogenic-driven acquired antibiotic resistance (AR). In contrast, accumulation of acquired AR is less probable in most Polar soils, providing a platform for characterizing background resistance and establishing a benchmark for assessing AR spread. Here, high-throughput qPCR and geochemistry were used to quantify the abundance and diversity of both antibiotic resistance genes (ARGs) and selected mobile genetic elements (MGEs) across eight soil clusters in the Kongsfjorden region of Svalbard in the High Arctic. Relative ARG levels ranged by over two orders of magnitude (10−6 to 10−4 copies/16S rRNA gene copy), and showed a gradient of potential human and wildlife impacts across clusters as evidenced by altered geochemical conditions and increased “foreign” ARG abundances (i.e., allochthonous), including blaNDM-1. Impacted clusters exhibited 100× higher total ARGs and MGEs in tandem with elevated secondary nutrients, especially available P that is typically low and limiting in Arctic soils. In contrast, ARGs in less-impacted clusters correlated strongly to local soil lithology. The most plausible source of exogenous P and allochthonous ARGs in this region is bird and other wildlife guano, disseminated either by local human wastes or via direct carriage and deposition. Regardless of pathway, accumulation of apparent allochthonous ARGs and MGEs in High Arctic soils is concerning, highlighting the importance of characterizing Arctic sites now to establish benchmarks for tracking AR spread around the world. Article in Journal/Newspaper Arctic Arctic Kongsfjord* Kongsfjorden Svalbard The University of Kansas: KU ScholarWorks Arctic Guano ENVELOPE(141.604,141.604,-66.775,-66.775) Svalbard Environment International 125 497 504 |
institution |
Open Polar |
collection |
The University of Kansas: KU ScholarWorks |
op_collection_id |
ftunivkansas |
language |
unknown |
topic |
High Arctic Antibiotic resistance International spread Wildlife Geochemistry High throughput qPCR |
spellingShingle |
High Arctic Antibiotic resistance International spread Wildlife Geochemistry High throughput qPCR McCann, Clare M. Christgen, Beate Roberts, Jennifer A. Su, Jian-Qiang Arnold, Kathryn E. Gray, Neil D. Zhu, Yong-Guan Graham, David W. Understanding drivers of antibiotic resistance genes in High Arctic soil ecosystems |
topic_facet |
High Arctic Antibiotic resistance International spread Wildlife Geochemistry High throughput qPCR |
description |
This work is licensed under a Creative Commons Attribution 4.0 International License. Soils in tropical and temperate locations are known to be a sink for the genetic potential of anthropogenic-driven acquired antibiotic resistance (AR). In contrast, accumulation of acquired AR is less probable in most Polar soils, providing a platform for characterizing background resistance and establishing a benchmark for assessing AR spread. Here, high-throughput qPCR and geochemistry were used to quantify the abundance and diversity of both antibiotic resistance genes (ARGs) and selected mobile genetic elements (MGEs) across eight soil clusters in the Kongsfjorden region of Svalbard in the High Arctic. Relative ARG levels ranged by over two orders of magnitude (10−6 to 10−4 copies/16S rRNA gene copy), and showed a gradient of potential human and wildlife impacts across clusters as evidenced by altered geochemical conditions and increased “foreign” ARG abundances (i.e., allochthonous), including blaNDM-1. Impacted clusters exhibited 100× higher total ARGs and MGEs in tandem with elevated secondary nutrients, especially available P that is typically low and limiting in Arctic soils. In contrast, ARGs in less-impacted clusters correlated strongly to local soil lithology. The most plausible source of exogenous P and allochthonous ARGs in this region is bird and other wildlife guano, disseminated either by local human wastes or via direct carriage and deposition. Regardless of pathway, accumulation of apparent allochthonous ARGs and MGEs in High Arctic soils is concerning, highlighting the importance of characterizing Arctic sites now to establish benchmarks for tracking AR spread around the world. |
format |
Article in Journal/Newspaper |
author |
McCann, Clare M. Christgen, Beate Roberts, Jennifer A. Su, Jian-Qiang Arnold, Kathryn E. Gray, Neil D. Zhu, Yong-Guan Graham, David W. |
author_facet |
McCann, Clare M. Christgen, Beate Roberts, Jennifer A. Su, Jian-Qiang Arnold, Kathryn E. Gray, Neil D. Zhu, Yong-Guan Graham, David W. |
author_sort |
McCann, Clare M. |
title |
Understanding drivers of antibiotic resistance genes in High Arctic soil ecosystems |
title_short |
Understanding drivers of antibiotic resistance genes in High Arctic soil ecosystems |
title_full |
Understanding drivers of antibiotic resistance genes in High Arctic soil ecosystems |
title_fullStr |
Understanding drivers of antibiotic resistance genes in High Arctic soil ecosystems |
title_full_unstemmed |
Understanding drivers of antibiotic resistance genes in High Arctic soil ecosystems |
title_sort |
understanding drivers of antibiotic resistance genes in high arctic soil ecosystems |
publisher |
Elsevier |
publishDate |
2021 |
url |
http://hdl.handle.net/1808/31064 https://doi.org/10.1016/j.envint.2019.01.034 |
long_lat |
ENVELOPE(141.604,141.604,-66.775,-66.775) |
geographic |
Arctic Guano Svalbard |
geographic_facet |
Arctic Guano Svalbard |
genre |
Arctic Arctic Kongsfjord* Kongsfjorden Svalbard |
genre_facet |
Arctic Arctic Kongsfjord* Kongsfjorden Svalbard |
op_relation |
Clare M. McCann, Beate Christgen, Jennifer A. Roberts, Jian-Qiang Su, Kathryn E. Arnold, Neil D. Gray, Yong-Guan Zhu, David W. Graham, "Understanding drivers of antibiotic resistance genes in High Arctic soil ecosystems", Environment International, Volume 125, 2019, Pages 497-504, ISSN 0160-4120, https://doi.org/10.1016/j.envint.2019.01.034. http://hdl.handle.net/1808/31064 doi:10.1016/j.envint.2019.01.034 |
op_rights |
© 2019 The Authors. Published by Elsevier Ltd. http://creativecommons.org/licenses/by/4.0/ openAccess |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1016/j.envint.2019.01.034 |
container_title |
Environment International |
container_volume |
125 |
container_start_page |
497 |
op_container_end_page |
504 |
_version_ |
1766298713323470848 |