Functional, Biochemical and Molecular Analyses of the *Cold Stable Eye Lens Crystallins From the Antarctic Toothfish Dissostichus Mawsoni
184 p. Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 2005. Isolation, cloning and sequencing of the cDNAs encoding for the toothfish crystallins revealed a total of 21 isoforms (2 alphas, 6 betas, and 13 gammas). Phylogenetic analyses suggest that toothfish alpha and beta crystallins a...
Main Author: | |
---|---|
Other Authors: | |
Format: | Text |
Language: | unknown |
Published: |
2005
|
Subjects: | |
Online Access: | http://hdl.handle.net/2142/84821 |
id |
ftunivillidea:oai:www.ideals.illinois.edu:2142/84821 |
---|---|
record_format |
openpolar |
spelling |
ftunivillidea:oai:www.ideals.illinois.edu:2142/84821 2023-05-15T13:33:27+02:00 Functional, Biochemical and Molecular Analyses of the *Cold Stable Eye Lens Crystallins From the Antarctic Toothfish Dissostichus Mawsoni Kiss, Andor J. DeVries, Arthur L. 2005 http://hdl.handle.net/2142/84821 unknown http://hdl.handle.net/2142/84821 (MiAaPQ)AAI3202116 Biology Zoology text 2005 ftunivillidea 2016-03-19T23:51:25Z 184 p. Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 2005. Isolation, cloning and sequencing of the cDNAs encoding for the toothfish crystallins revealed a total of 21 isoforms (2 alphas, 6 betas, and 13 gammas). Phylogenetic analyses suggest that toothfish alpha and beta crystallins are orthologues of mammalian counterparts, while gammas do not. Further separation, partial purification (ion-exchange chromatography) and analyses (isoelectric focussing and 2D SDS-PAGE) of the gamma crystallins has generated eleven sub-fractions, although they are not homogeneous for a single gamma isoform. Cross-species chaperone-like assays with these eleven toothfish gamma fractions show that those containing acidic gammas are protected by cow alpha crystallin, while those which are largely composed of basic isoforms cannot. Solubility of toothfish lens alpha, beta and gamma crystallins are similar to which has been previously reported for cow and humans. Thus, the long-lived, cold-adapted Antarctic toothfish lens is an attractive model system for further investigations into lens crystallin stability. Text Antarc* Antarctic Antarctic Toothfish University of Illinois at Urbana-Champaign: IDEALS (Illinois Digital Environment for Access to Learning and Scholarship) Antarctic The Antarctic |
institution |
Open Polar |
collection |
University of Illinois at Urbana-Champaign: IDEALS (Illinois Digital Environment for Access to Learning and Scholarship) |
op_collection_id |
ftunivillidea |
language |
unknown |
topic |
Biology Zoology |
spellingShingle |
Biology Zoology Kiss, Andor J. Functional, Biochemical and Molecular Analyses of the *Cold Stable Eye Lens Crystallins From the Antarctic Toothfish Dissostichus Mawsoni |
topic_facet |
Biology Zoology |
description |
184 p. Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 2005. Isolation, cloning and sequencing of the cDNAs encoding for the toothfish crystallins revealed a total of 21 isoforms (2 alphas, 6 betas, and 13 gammas). Phylogenetic analyses suggest that toothfish alpha and beta crystallins are orthologues of mammalian counterparts, while gammas do not. Further separation, partial purification (ion-exchange chromatography) and analyses (isoelectric focussing and 2D SDS-PAGE) of the gamma crystallins has generated eleven sub-fractions, although they are not homogeneous for a single gamma isoform. Cross-species chaperone-like assays with these eleven toothfish gamma fractions show that those containing acidic gammas are protected by cow alpha crystallin, while those which are largely composed of basic isoforms cannot. Solubility of toothfish lens alpha, beta and gamma crystallins are similar to which has been previously reported for cow and humans. Thus, the long-lived, cold-adapted Antarctic toothfish lens is an attractive model system for further investigations into lens crystallin stability. |
author2 |
DeVries, Arthur L. |
format |
Text |
author |
Kiss, Andor J. |
author_facet |
Kiss, Andor J. |
author_sort |
Kiss, Andor J. |
title |
Functional, Biochemical and Molecular Analyses of the *Cold Stable Eye Lens Crystallins From the Antarctic Toothfish Dissostichus Mawsoni |
title_short |
Functional, Biochemical and Molecular Analyses of the *Cold Stable Eye Lens Crystallins From the Antarctic Toothfish Dissostichus Mawsoni |
title_full |
Functional, Biochemical and Molecular Analyses of the *Cold Stable Eye Lens Crystallins From the Antarctic Toothfish Dissostichus Mawsoni |
title_fullStr |
Functional, Biochemical and Molecular Analyses of the *Cold Stable Eye Lens Crystallins From the Antarctic Toothfish Dissostichus Mawsoni |
title_full_unstemmed |
Functional, Biochemical and Molecular Analyses of the *Cold Stable Eye Lens Crystallins From the Antarctic Toothfish Dissostichus Mawsoni |
title_sort |
functional, biochemical and molecular analyses of the *cold stable eye lens crystallins from the antarctic toothfish dissostichus mawsoni |
publishDate |
2005 |
url |
http://hdl.handle.net/2142/84821 |
geographic |
Antarctic The Antarctic |
geographic_facet |
Antarctic The Antarctic |
genre |
Antarc* Antarctic Antarctic Toothfish |
genre_facet |
Antarc* Antarctic Antarctic Toothfish |
op_relation |
http://hdl.handle.net/2142/84821 (MiAaPQ)AAI3202116 |
_version_ |
1766042413150765056 |